Clinical observations on the course of oxytocinor prostaglandin E2/oxytocin-induced parturition in mares

Witkowski, M.; Pawłowski, Karol

doi:10.2478/pjvs-2014-0047

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…Until now, many studies have been focusing on the different application route or sites of luteolytic/luteotropic factors that may be used in veterinary practices to regulate the estrous cycle in mares. In the literature, different ways of PGE 2 or hCG administrations have been demonstrated, for example, i.m., i.v., s.c., intrafollicular, or intracervical (10,14,17,18,58,59). The ultrasound-guided intra-CL injection as a method for studying the direct effect of PGF 2α on reproductive function in mares was evaluated by Weber et al (61).…”

Section: Discussionmentioning

confidence: 99%

“…While some studies reported intrafollicular PGE 2 administration induced ovulation ( 58 ), in other studies intrauterine (intra-U) administration of PGE 2 resulted in prolonged CL ( 32 ). Moreover, the positive influence of intracervical administration of PGE 2 on the preparation of the uterine cervix to parturition in mares has been observed ( 59 ).…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

et al. 2022

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We examined the effect of prostaglandin (PG) E2 on the secretory function of equine corpus luteum (CL), according to the application site: intra-CL injection vs. an intrauterine (intra-U) administration. Moreover, the effect of intra-CL injection vs. intra-U administration of both luteotropic factors: PGE2 and human chorionic gonadotropin (hCG) as a positive control, on CL function was additionally compared. Mares were assigned to the groups (n = 6 per group): (1) an intra-CL saline injection (control); (2) an intra-CL injection of PGE2 (5 mg/ml); (3) an intra-CL injection of hCG (1,500 IU/ml); (4) an intra-U saline administration (control); (5) an intra-U administration of PGE2 (5 mg/5 ml); (6) an intra-U administration of hCG (1,500 IU/5 ml). Progesterone (P4) and PGE2 concentrations were measured in blood plasma samples collected at −2, −1, and 0 (pre-treatment), and at 1, 2, 3, 4, 6, 8, 10, 12, and 24 h after treatments. Moreover, effects of different doses of PGE2 application on the concentration of total PGF2α (PGF2α and its main metabolite 13,14-dihydro-15-keto-prostaglandin F2α– PGFM) was determined. The time point of PGE2, hCG, or saline administration was defined as hour “0” of the experiment. An intra-CL injection of PGE2 increased P4 and PGE2 concentrations between 3 and 4 h or at 3 and 12 h, respectively (p < 0.05). While intra-U administration of PGE2 elevated P4 concentrations between 8 and 24 h, PGE2 was upregulated at 1 h and between 3 and 4 h (p < 0.05). An intra-CL injection of hCG increased P4 concentrations at 1, 6, and 12 h (p < 0.05), while its intra-U administration enhanced P4 and PGE2 concentrations between 1 and 12 h or at 3 h and between 6 and 10 h, respectively (p < 0.05). An application of PGE2, dependently on the dose, supports equine CL function, regardless of the application site, consequently leading to differences in both P4 and PGE2 concentrations in blood plasma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

et al. 2022

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“…Regardless of whether parturients underwent premature labor or TL, the processes of initiation of delivery were the same, which included regular uterine contraction, dilatation of cervix and rupture of membranes, all of which involve the important role of PG [12,13]. The main function of PG is to stimulate smooth muscle cells of uterine wall, causing contraction of muscles which then induce the initiation of labor.…”

Section: Discussionmentioning

confidence: 99%

Expression of hydrogen sulfide, hydrogen-sulfide synthase and cyclooxygenase-2, and their mechanisms of action in amniotic tissues

Liu¹,

Liú²,

Duan³

et al. 2018

Trop. J. Pharm Res

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Purpose:To investigate the expressions of cystathionine β-synthase (CBS), cystathionine γ -lyase (CSE) and cyclooxygenase -2 (COX -2) and their relationships with premature delivery in amniotic tissues. Methods: Parturients were divided into three groups: 40 preterm-labor parturients (PTL group), 28 term-labor parturients (TL group), and 28 term non-labor parturients (TNL group). Changes in expressions of CBS, CSE and COX-2 were determined by Western blot (WB) in amniotic tissues of parturients in the three groups. The expression of COX -2 was determined after the amniotic tissues of parturients in the TNL group were cultured in vitro and processed by exogenous hydrogen-sulfide (H2S). Results: The expression level of COX -2 was significantly lower in the TNL group, when compared with the PTL and TL groups (p < 0.05). The expression of CBS was increased in the order PTL > TL > TNL, with TNL group having the highest level, and there were significant differences in CBS expressions among the three groups (p < 0.05). Conclusion: These results suggest that when the expressions of CBS and CSE are down-regulated, the decreased H2S synthesis promotes the overexpression of COX -2 by NF -kB signaling pathway, causing increased prostaglandin synthesis which results in premature delivery. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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“…The current thinking on PGE 2 and PGF 2α in reproduction emphasizes not only their uterotonic roles, but also their proinflammatory effects. In addition to being a uterotonic 7 , PGE 2 causes cervical ripening in humans 37 , and it has been effective as a drug for opening the cervix in horses 38 . It increases pain, swelling, and temperature.…”

mentioning

confidence: 99%

The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention

Rapacz-Leonard

Leonard

Chmielewska-Krzesińska

et al. 2020

Sci Rep

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Despite their importance in mammalian reproduction, substances in the oxytocin-prostaglandins pathways have not been investigated in the horse placenta during most of pregnancy and parturition. Therefore, we quantified placental content of oxytocin (OXT), oxytocin receptor (OXTR), and prostaglandin E2 and F2 alpha during days 90-240 of pregnancy (PREG), physiological parturition (PHYS), and parturition with fetal membrane retention (FMR) in heavy draft horses (PREG = 13, pHYS = 11, FMR = 10). We also quantified OXTR and prostaglandin endoperoxide synthase-2 (PTGS2) mRNA expression and determined the immunolocalization of OXT, OXTR, and PTGS2. For relative quantification of OXT and OXTR, we used western blotting with densitometry. To quantify the prostaglandins, we used enzyme immunoassays. For relative quantification of OXTR and PTGS2, we used RT-qPCR. For immunolocalization of OXT, OXTR, and PTGS2, we used immunohistochemistry. We found that OXT was present in cells of the allantochorion and endometrium in all groups. PTGS2 expression in the allantochorion was 14.7-fold lower in FMR than in PHYS (p = 0.007). These results suggest that OXT is synthesized in the horse placenta. As PTGS2 synthesis is induced by inflammation, they also suggest that FMR in heavy draft horses may be associated with dysregulation of inflammatory processes. In the placenta, a delicate balance of hormones helps to support pregnancy and initiate parturition 1-3. Parturition is a proinflammatory event 4-8 , and delivery of the fetus and the placenta are accompanied by coordinated contractions, pain, and swelling, which are mostly mediated by prostaglandins 9. Exposure of the placenta to endogenous or exogenous oxytocin (OXT) causes it to release prostaglandin E2 (PGE 2) and prostaglandin F2 alpha (PGF 2ɑ) within a few hours 10-21 (see Supplementary Fig S1 for the pathways that start with OXT release and end with the prostaglandins binding to their receptors). OXT is released from the posterior pituitary gland 22 , and during pregnancy and parturition in humans, from the placenta 23. Given that, of all domestic and laboratory species, horses may be the most similar to humans in terms of the endocrinology of pregnancy and parturition 24 , it seems likely that horses also release OXT from the placenta. Moreover, human placental OXT is not believed to play a role in myometrial contractions, unlike pituitary OXT 13. Thus, it seems likely that horse placental OXT would be a signal for prostaglandin release in the placenta itself, and not a signal for myometrial contractions. However, the presence of OXT in horse placental cells needs to be verified.

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Clinical observations on the course of oxytocinor prostaglandin E2/oxytocin-induced parturition in mares

Cited by 6 publications

References 14 publications

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

The Effects of Prostaglandin E2 Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study

Expression of hydrogen sulfide, hydrogen-sulfide synthase and cyclooxygenase-2, and their mechanisms of action in amniotic tissues

The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention

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