Clinical multi-colour fluorescence imaging of malignant tumours - initial experience

Svanberg, Katarina; Wang, I; Colleen, S.; Idvall, Ingrid; Ingvar, Christian; Rydell, Roland; Jocham, Dieter; Diddens, H.; Bown, SG; Gregory, George; Montán, S.; Andersson‐Engels, Stefan; Svanberg, Sune

doi:10.1080/02841859809172141

Cited by 72 publications

(38 citation statements)

References 16 publications

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“…This has been proposed previously for mammary tumours after i.v. application of Photofrin (Svanberg et al, 1998). The qualities of ALA however, make a clinical application for PDD more feasible.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Ladner

Steiner²,

Allemann³

et al. 2001

Br J Cancer

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Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic diagnosis of human primary mammary tumours. The study included 16 patients with palpable breast tumours. Aminolevulinic acid was administered at a concentration of 40 mg kg −1 bodyweight 150–420 min prior to tumourectomy. Intraoperatively blue light (405 nm) was applied to the operation site. Sections of the excised tumour and some lymph nodes were prepared and analysed with a fluorescent microscope. All primary mammary tumour tissues showed significantly higher fluorescence intensity than surrounding normal mammary tissue. Fluorescence of the mammary tumours could also be discriminated macroscopically and intraoperatively. Fluorescence intensity in nonmetastatic lymph node tissue was higher in 2 out of 3 patients than in primary tumour tissue. By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients. We suggest that photodynamic diagnosis with aminolevulinic acid for breast tumours should be further investigated and developed for intraoperative use and may well be a simple tool for better intraoperative diagnosis and recognition of tumour margins. We hypothesize that lymph node metastasis of breast tumours will not be detectable by this method. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Section: Discussionmentioning

confidence: 99%

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Ladner

Steiner²,

Allemann³

et al. 2001

Br J Cancer

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show abstract

“…3,4 It can be applied in point monitoring 5 or in imaging applications. 6,7 Applications include diagnostics of human malignant tumors 8,9 and atherosclerotic disease, 10 vegetation and water monitoring, 11,12 analytical chemistry techniques such as HPLC and capillary electrophoresis, 13 and forensic sciences. 14 Fluorescence diagnostics has been largely simplified through the availability of CCD detectors for direct readout of the spectrum obtained in the image plane of a spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

Gustafsson

Pålsson

Svanberg

2000

Review of Scientific Instruments

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A compact fluorosensor with a fiber-optic measurement probe was developed, employing a continuous-wave violet diode laser as an exciting source and an integrated digital spectrometer for the monitoring of fluorescence signatures. The system has the dimensions 22×13×8 cm3, and features 5 nm spectral resolution and an excellent detectivity. Results from measurements on vegetation and human premalignant skin lesions are reported, illustrating the potential of the instrument.

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“…A slightly more advanced system using splitting optics to provide four images of an object, filtered at different emission bands has also been developed (Montán et al 1985, Andersson-Engels et al 1990, 1994, 1995, Svanberg et al 1997. The four images can then be computer processed for viewing of the optimized contrast function image.…”

Section: Fluorescence Imaging Techniquesmentioning

confidence: 99%

In vivofluorescence imaging for tissue diagnostics

et al. 1997

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In vivo fluorescence imaging for tissue diagnosticsAndersson-Engels, Stefan; af Klinteberg, C; Svanberg, Katarina; Svanberg, Sune General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. Non-invasive fluorescence imaging has the potential to provide in vivo diagnostic information for many clinical specialities. Techniques have been developed over the years for simple ocular observations following UV excitation to sophisticated spectroscopic imaging using advanced equipment. Much of the impetus for research on fluorescence imaging for tissue diagnostics has come from parallel developments in photodynamic therapy of malignant lesions with fluorescent photosensitizers. However, the fluorescence of endogenous molecules (tissue autofluorescence) also plays an important role in most applications. In this paper, the possibilities of imaging tissues using fluorescence spectroscopy as a mean of tissue characterization are discussed. The various imaging techniques for extracting diagnostic information suggested in the literature are reviewed. The development of exogenous fluorophores for this purpose is also presented. Finally, the present status of clinical evaluation and future directions are discussed. In vivo fluorescence imaging for tissue diagnostics

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Clinical multi-colour fluorescence imaging of malignant tumours - initial experience

Abstract: The initial clinical experience of using multi-colour fluorescence imaging has shown that the technique has the potential to reveal malignant tumour tissue, including non-invasive early carcinoma and also precancerous tissue. Further investigations are needed to fully develop the method.

Cited by 72 publications

References 16 publications

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Compact fiber-optic fluorosensor using a continuous-wave violet diode laser and an integrated spectrometer

In vivofluorescence imaging for tissue diagnostics

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