Clinical, microbiological and immunological features of subjects with refractory periodontal diseases

Abstract

27 subjects with active destructive periodontal diseases were treated by modified Widman flap surgery and systemic tetracycline and divided into 4 groups based on pre- and post-therapy hazard rates (% of sites losing greater than 3 mm of attachment in 1 year). Pre- and post-therapy hazard rates were respectively: group I (3 subjects) less than 4 and less than 4; group II (8 subjects) greater than 4 and less than 4; group III (3 subjects) less than 4 and greater than 4; group IV (refractory group of 13 subjects) greater than 4 and greater than 4. Baseline mean pocket depths and attachment loss of groups I and II subjects were less than groups III and IV subjects and exhibited less suppuration. 6 group IV subjects lost a total of 38 teeth after therapy, in contrast to no tooth loss in subjects in the other 3 groups. Redness, bleeding on probing, plaque levels and age did not differ among groups. Subjects in the 4 groups differed in the subgingival species to which they showed elevated serum antibody responses. Group IV subjects showed elevated responses to a select range of gram-negative species, including A. actinomycetemcomitans strains Y4 or ATCC 29523, F. nucleatum and B. intermedius. No subject in any of the other groups exhibited an elevated response to B. intermedius. The mean % of each species in all sampled sites, both before and after therapy, was computed for each subject. Subjects in groups III and IV (high post-therapy hazard rates) exhibited elevated mean levels of B. forsythus, F. nucleatum, S. intermedius, E. corrodens, and B. gingivalis.(ABSTRACT TRUNCATED AT 250 WORDS)

Th1 biased response to a novel Porphyromonas gingivalis protein aggravates bone resorption caused by this oral pathogen

“…In the oral cavity, Porphyromonas gingivalis is a consensus oral pathogen that has been implicated in periodontal and periapical diseases in humans and other species [1,2,3,4]. In all of these models, infection with P. gingivalis elicits a cell-mediated Th1 type response characterized by increased production of IFN-γ, IL-12 and TNFα, and leads to increased inflammation and bone destruction [5,6].…”

Serum Antibody Response to Antigens of Oral Gram-negative Bacteria by Cats with Plasma Cell Gingivitis-Pharyngitis

“…1984) have been implicated (Frost and Williams, 1986), but it is clear that neither virus is found in all affected cats. The bacterial etiology of gingivitis and periodontitis in humans and in all other animals in which these diseases have been studied is firmly established, and specific species of predominantly Gram-negative anaerobes including Actinobacillus actinomycetemcomitans and various species of Bacteroides have been implicated (Page and Schroeder, 1982;Moore, 1987;Haffajee et al, 1988). A similar etiology seems likely in cats, although evidence is lacking.…”

Bacteroides forsythus: sensibilidade a antimicrobianos em amostras de pacientes portadores de periodontite

“…Tem sido associado à periodontite do adulto, periodontite de progressão rápida e a casos de doença periodontal considerados refratários 4,8,9,10 . A presença de Bacteroides forsythus na microbiota subgengival é considerada um fator de risco para doença periodontal, aumentando o risco de perda de inserção em 2,5 vezes 6 .…”

“…A presença de Bacteroides forsythus na microbiota subgengival é considerada um fator de risco para doença periodontal, aumentando o risco de perda de inserção em 2,5 vezes 6 . Além disso, esta bactéria foi encontrada em maior número em sítios com doença periodontal avançada do que em sítios sadios 8 . Outro aspecto relevante foi observado por DZINK et al 3 (1988), quanto à capacidade de invasão dos tecidos periodontais por este microrganismo.…”

“…Outras metodologias empregadas para a identificação de B. forsythus, como testes imunodiagnósticos 4,5,8,9 , sondas de DNA e RNA e Reação de Polimerase em Cadeia (PCR) 2,11 têm demonstrado vantagens sobre a cultura deste microrganismo, mas tais testes ainda não são capazes de fornecer a sensibilidade do microrganismo frente aos antimicrobianos disponíveis.…”