Clinical metagenomic analysis of bacterial communities in breast abscesses of granulomatous mastitis

International Journal of Infectious Diseases

et al. 2019

Objective: Granulomatous lobular mastitis (GLM) is a rare inflammatory disease of the breast that clinically mimics breast cancer. However, its etiology is not completely defined. The purpose of this study was to systematically study the bacteriology of GLM using advanced detection technology. Methods: Paraffin-embedded tissue from patients with GLM was collected. DNA was extracted from the samples and analyzed using next-generation sequencing (NGS) technology, and the data were processed using bioinformatics analyses. Results: A total of 40 patients were recruited into the study. A bioinformatics analysis revealed that a total of 17 genera or 19 species of pathogens were present in 39 of the GLM patients (97.5%). These included bacteria, fungi, and Mycobacterium tuberculosis complex group. Bacteria were found in 39 of the patient cases, while fungi were present in five. Only one case tested positive for M. tuberculosis complex. In addition, a single genus of pathogen was found in nine patients (23.1%), whereas 30 patients (76.9%) tested positive for multiple pathogens. Conclusions: This study profiled the microbiota of patients with GLM using NGS technology, which provides more useful information for establishing patient treatment plans.

Section: Discussionsupporting

confidence: 57%

Pathogens in patients with granulomatous lobular mastitis

International Journal of Infectious Diseases

et al. 2019

“…All patients were managed conservatively with systemic antibiotics consisting of augmentin (1 mg twice a day) for 6 weeks and metronidazole (400 mg three times a day) for two weeks, and 17 out of 20 patients (85%) showed significant improvement. The difference in antibiotic efficacy between studies may be due to accidental or specific types of antibiotics used by [20,21]. Based on this consideration, in our treatment design, we used hydrogen peroxide, iodophor, and metronidazole to irrigate the lesions during and after surgery, and we achieved good results.…”

Section: Discussionmentioning

confidence: 99%

Minimally invasive comprehensive treatment for granulomatous lobular mastitis

Song

et al. 2020

BMC Surg

Objective: To describe a minimally invasive comprehensive treatment for granulomatous lobular mastitis (GLM) and compare its effect with the existing methods, particularly in terms of its recurrence rate and esthetic outcomes. Methods: This retrospective study reviewed 69 GLM patients receiving the minimally invasive comprehensive treatment. Patients' information, including age, clinical features, image characteristics, histopathological findings, mastitis history, treatment process, operative technique, recurrence, and esthetic effect, was evaluated. Results: All patients were female with a median age of 32 (range 17-55) years. Hospital stays ranged from 2 to 34 days, with a median of 6 days. The shortest time for complete rehabilitation was 2 days and the longest time was 365 days, with a median of 30 days. After a median follow-up of 391 days (range 162-690), 7 patients (10.14%) relapsed. The average cosmetic score was 2.62 ± 0.57 points and was mainly related to the past treatment, especially the surgical history. Conclusion: Minimally invasive comprehensive treatment is a new method for the treatment of GLM, ensuring a therapeutic effect while maintaining breast beauty.

“…It is mainly used for systemic or local infection caused by anaerobic bacteria, especially for Bacteroides, Clostridium, some Eubacteria, digestive cocci, and digestive Streptococcus. Some researchers believe that the occurrence of GLM is related to anaerobic bacteria, especially Corynebacterium [14,15].…”

Section: Discussionmentioning

confidence: 99%

Minimally invasive comprehensive treatment for granulomatous lobular mastitis

Song

et al. 2020

Preprint

Objective: To describe a minimally invasive comprehensive treatment for granulomatous lobular mastitis (GLM) and compare its effect with the existing methods, particularly in terms of its recurrence rate and esthetic outcomes. Methods: This retrospective study reviewed 69 GLM patients receiving the minimally invasive comprehensive treatment. Patients’ information, including age, clinical features, image characteristics, histopathological findings, mastitis history, treatment process, operative technique, recurrence, and esthetic effect, was evaluated. Results: All patients were female with a median age of 32 (range 17 - 55) years. Hospital stays ranged from 2 to 34 days, with a median of 6 days. The shortest time for complete rehabilitation was 2 days and the longest time was 365 days, with a median of 30 days. After a median follow-up of 391 days (range 162 - 690), 7 patients (10.14%) relapsed. The average cosmetic score was 2.62 ± 0.57 points and was mainly related to the past treatment, especially the surgical history. Conclusion: Minimally invasive comprehensive treatment is a new method for the treatment of GLM, ensuring a therapeutic effect while maintaining breast beauty.