Clinical isolation of Nocardia cyriacigeorgica from patients with various clinical manifestations, the first report from Iran

Shojaei, Hasan; Hashemi, Abdolrazagh; Heidarieh, Parvin; Eshraghi, Saeid; Khosravi, Alireza; Naser, Abass Daei

doi:10.3314/jjmm.52.39

Cited by 12 publications

(12 citation statements)

References 14 publications

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“…Nocardiosis in Iran and other parts of the world in clinical forms of pulmonary, sinusitis and mycetoma has been reported [32][33][34][35][36][37][38][39] . Also, like the study performed by Stapleton et al, in this study it was found that the rate of isolation of Nocardia spp.…”

Section: Discussionmentioning

confidence: 99%

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Kachuei

Emami

Mirnejad

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Section: Discussionmentioning

confidence: 99%

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Kachuei

Emami

Mirnejad

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…Current study like other reports revealed that pulmonary and disseminated nocardial disease is observed mostly in immune-compromised patients, whereas primary cutaneous nocardiosis is usually an infection of immune-compe- tent hosts [16]- [19]. In recent years, nocardiosis has been increasingly reported in Iran [20]- [23], however in most of them identification of the clinical isolates were determined on the basis of microscopic examination, culturing and sometimes phenotypic tests. Twelve of the isolates (26%] were identified as N. asteroids as the most frequent encounter species in clinical samples by molecular methods.…”

Section: Discussionmentioning

confidence: 94%

“…The second most dominant species in our clinical isolates was N. cyriacigeorgica (9 isolates, 19.5%). Infection due to this species was previously reported from Iran based on phenotypic methods and 16S rRNA sequencing [23]. Disease due to this species has recently been reported [25] and it represents 10.2% and 18% of the Nocardia isolates in Japan and Thailand [26] and USA [27] respectively and 22% and 32.5% from two different studies from Spain [16] [24].…”

Section: Discussionmentioning

confidence: 99%

Species Spectrum of <I>Nocardia</I> spp. Isolated from Suspected Tuberculosis Patients

Shahraki¹,

Bostanabad²,

Heidarieh³

et al. 2015

Health

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Traditionally Nocardia asteroides was considered the predominant species of causing nocardiosis. The improved identification of isolates using molecular techniques have shown that the genus exhibits considerable taxonomic complexity and phenotypic base identification can be ambiguous. The aim of this study was to assess the species distribution of Nocardia strains mostly recovered from patients suspected of having tuberculosis, during three years period (2009-2012). The clinical isolates were identified to species level using conventional tests and genotypic methods using single and multi locus sequence analysis (MLSA) of 16S rRNA, gyrB and secA1 genes. Nocardiosis was diagnosed in 46 patients. The most frequent underlying condition were organ transplantation (6 patients; 13%), cancer (6 patients; 13%), human immunodeficiency virus (HIV) (6 patients; 13%), non-infectious chronic lung disease (5 patients; 10.8%) and tuberculosis (4 patients; 8.7%). Nocardia species was recovered from 46 different clinical specimens, the most common of which was bronchoalveolar lavage (BAL) (43.5%). Eleven different Nocardia species were identified: N. asteroides (n = 12), N. cyriacigeorgica (n = 9), N. farcinica (n = 7), N. wallacei (n = 6), N. carnea (n = 3), N. otitidiscaviarum (n = 3), N. abscessus (n = 1), N. arthritidis (n = 1), N. kruczakiae (n = 1), N. nova (n = 2) and N. veterana (n = 1). In conclusion, infection caused by Nocardia species appears to be more common than generally appreciated. The current study provides further evidence that Nocardia species are capable of causing a wide range of human diseases in healthy and immunocompromised patients. MLSA is a reliable method for accurate species identification of Nocardia * The GenBank accession numbers of investigated clinical isolates of Nocardia determined in this work are as follows: KC262083-KC262128, KC262130-KC262175 and KC296657-KC296702 for the 16S rRNA, gyrB and secA1, respectively. # Corresponding author.A. Hashemi-Shahraki et al. 848isolates and would be more feasible for routine use in clinical laboratories.

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“…N. asteroides was the most frequently recovered, followed by N. farcinica and N. cyriacigeorgica (9). Other reports have shown N. cyriacigeorgica, N. asteroides complex, N. nova complex, N. otitidiscaviarum, and N. transvalensis in Iran (10)(11)(12)(13). The prevalence of Nocardial infections in another study was 5.28% (6).…”

Section: Introductionmentioning

confidence: 84%

Molecular Identification of Nocardia Strains from the Soil by hsp65 Gene: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP) and Analysis of Sequence 16S rRNA Gene

Nasab

Habibnia

Heidarieh

et al. 2018

Arch Clin Infect Dis

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Background: Nocardia species are nocardiosis agents particularly in immune disorder patients with the clinical manifestations including fatigue, malaise, weight loss, cough, and dyspnoea. Due to time -consuming bacterial culture and insufficient phenotypic tests alone, rapid molecular assays for the diagnosis of Nocardia are essential. The primary objective of this study was the analysis of the polymerase chain reaction -restriction fragment length polymorphism (PCR -RFLP) and PCR sequencing of 16S rRNA gene for the identification and differentiation of Nocardia species. Methods: 32 Nocardia isolates were identified by single digestion of 441 -nt fragments of the 65 -heat -shock protein gene with endonucleases BstEII, MspI, and HinfI and sequencing of 16S rRNA gene. These isolates were collected from the soil of various geographical regions of Iran by paraffin baiting technique in our previous study. Results: Some of the isolates had new patterns that were not identified by PCR -RFLP of hsp65 gene in literature; therefore, they were identified by sequence analysis of 16S rRNA gene. Six strains of Nocardia with new DNA banding patterns in PCR -RFLP of hsp65 (A and B) could not be identified by their hsp65 restriction -endonucleases fragment patterns. Strain En49 after analysis of sequencing data was recognized as Nocardia coubleae, which its PCR -RFLP profile of hsp65 has not been reported in the literature so far. Conclusions:The PCR -RFLP -hsp65 is faster than other sequencing-based techniques, but species accurate identification in strains with new PCR -RFLP profiles is not possible. The 16S rRNA gene sequencing is a suitable method to determine the percent similarity and phylogenetic relationships of Nocardia species.

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Clinical isolation of Nocardia cyriacigeorgica from patients with various clinical manifestations, the first report from Iran

Cited by 12 publications

References 14 publications

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Species Spectrum of <I>Nocardia</I> spp. Isolated from Suspected Tuberculosis Patients

Molecular Identification of Nocardia Strains from the Soil by hsp65 Gene: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP) and Analysis of Sequence 16S rRNA Gene

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Clinical isolation of Nocardia cyriacigeorgica from patients with various clinical manifestations, the first report from Iran

Cited by 12 publications

References 14 publications

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Diversity and frequency of Nocardia spp. in the soil of Isfahan province, Iran

Species Spectrum of &lt;I&gt;Nocardia&lt;/I&gt; spp. Isolated from Suspected Tuberculosis Patients

Molecular Identification of Nocardia Strains from the Soil by hsp65 Gene: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP) and Analysis of Sequence 16S rRNA Gene

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Species Spectrum of <I>Nocardia</I> spp. Isolated from Suspected Tuberculosis Patients