Clinical isolates of Staphylococcus aureus have osteolytic surface proteins and a proportion of the population have antibodies that block this activity: is this of prognostic significance?

Nair, Sean P.; Meghji, S; Wilson, May G.; Nugent, I M; Ross, A.G.; Ismael, Alaa B.; Bhudia, N K; Harris, Mary Catherine; Henderson, Brian E.

doi:10.1093/rheumatology/36.3.328

Cited by 14 publications

(15 citation statements)

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“…Such`virulence' factors are obvious targets for therapeutic intervention by various means, including vaccination. In an earlier study [9] we examined the sera from a small number of patients with S. aureus bone or joint infections and from uninfected healthy individuals. To our surprise we found no major di¡erence between the two groups in antibody titres to the secreted macromolecules of this bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…Of signi¢cance was the ¢nding that a proportion of the sera from infected and uninfected patients was able to block the bone resorption induced by the secreted macromolecular fraction produced by S. aureus. This raised the question as to whether such patients had been`vaccinated' against S. aureus-induced bone destruction [9]. We attempted to analyse the nature of the antigens recognised by the IgG in the sera of this small number of patients, but no signi¢cant di¡eren-ces were apparent.…”

Section: Discussionmentioning

confidence: 99%

“…The titres of serum IgG antibodies to the secreted components of S. aureus were determined using the methodology described in [9]. To determine antibody titres to whole S. aureus the bacteria that had been washed to remove the cell-associated secreted components were lyophilised and resuspended at a concentration of 1 mg freeze-dried material ml 31 PBS.…”

Section: Elisa To Determine Titres Of Igg Antibodiesmentioning

confidence: 99%

“…A second blocking step, in which wells were incubated with 200 Wl of a 1:400 dilution of rabbit immunoglobulin fraction (Dako, Denmark), was included to block the protein A present on the surface of S. aureus. The remaining details of the assay are described in [9].…”

Section: Elisa To Determine Titres Of Igg Antibodiesmentioning

confidence: 99%

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Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Royan

2000

FEMS Immunology and Medical Microbiology

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The ability of sera to recognise secreted macromolecules of Staphylococcus aureus was examined by ELISA and Western immunoblotting. Individual secreted proteins were also studied using both human sera and sera from rabbits immunised with secreted macromolecules. Patients sera showed a wide range of IgG antibody titres to secreted macromolecules and whole bacteria. Controls showed a significantly lower IgG response. Western immunoblotting revealed that a significant number of secreted proteins were recognised by circulating IgG antibodies. Surprisingly, both the sera from controls and from patients recognised similar macromolecules including a number of potential virulence factors. The major difference was in the IgG binding to a 16-kDa component, which was recognised by the majority of the sera from infected individuals, but only by a small number of sera from healthy controls. The higher incidence of antibodies recognising the 16 kDa component may be related to our earlier finding that the major bone resorbing component of S. aureus is a heterodimeric protein containing a 16-kDa subunit, the activity of which could be blocked by sera. ß

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Elisa To Determine Titres Of Igg Antibodiesmentioning

confidence: 99%

Section: Elisa To Determine Titres Of Igg Antibodiesmentioning

confidence: 99%

See 2 more Smart Citations

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Royan

2000

FEMS Immunology and Medical Microbiology

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“…These surface-associated proteins and capsular material appear to promote the formation and activation of the bone-resorbing osteoclast (Lau et al, 2006; Meghji et al, 1998). Interestingly, a proportion of the population have antibodies that can block the action of the S. aureus proteins and prevent bone breakdown (Nair et al, 1997). The identity of the protein(s) in these mixtures which cause bone destruction has not been elucidated.…”

Section: Staphylococcal Virulence Determinantsmentioning

confidence: 99%

Interaction of staphylococci with bone

Wright

Nair

2010

International Journal of Medical Microbiology

220

166

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Staphylococci, in particular Staphylococcus aureus, are the predominant cause of bone infections worldwide. These infections are painful, debilitating and with the rise in antibiotic-resistant forms, increasingly difficult to treat. The growth in the number of prosthetic joint replacement procedures also provides new opportunities for these infections to take hold. Comprehending the mechanisms by which staphylococci interact with and damage bone is critical to the development of new approaches to meet this challenge. This review summarises current understanding of the mechanisms by which staphylococci infect and damage bone. We address the role of the inflammatory response to staphylococcal infection in disrupting the homeostatic balance of bone matrix deposition and resorption and thereby mediating bone destruction. A number of virulence factors that have been shown to contribute to bone infection and pathology are discussed, however no single factor has been defined as being specific to bone infections. Although traditionally considered an extracellular pathogen, there is increasing evidence that staphylococci are able to invade host cells, and that an intracellular lifestyle may facilitate long-term persistence in bone tissue, enabling evasion of antimicrobials and host immune responses. ‘Small colony variant’ strains, with mutations disabling the electron transport pathway appear particularly adept at invading and persisting within host cells, and exhibit enhanced antimicrobial resistance, and may represent a further complication in the treatment and management of staphylococcal bone disease.

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The local inflammatory environment and microorganisms in “aseptic” loosening of hip prostheses

2007

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Long term loosening of hip prostheses remains an important problem in orthopedics. Although various loosening mechanisms have been proposed, the exact process is still unclear. Particle disease and the pressure theory are widely known and generally accepted hypotheses to explain long term implant failure. Each proposed mechanism recognizes a local inflammatory response in which macrophages represent the main cell-type and several proinflammatory and antiinflammatory cytokines (IL-1beta, IL-6, TNFalpha, IL-10, TGFbeta), chemokines (IL-8/CXCL8, MCP-1/CCL2, RANTES/CCL5, MIP-1alpha/CCL3) and other mediators (GM-CSF, M-CSF, MMP-1, PDGF-alpha, PGE(2), IL-11) are identified. The cytokines have different functions and some are capable of stimulating bone resorption in various ways; either directly or indirectly. Even though the implant loosening is thought to be "aseptic", several studies suggested a possible role for bacteria and a bacterial biofilm in implant failure. Biofilm-derived bacteria and bacterial products might have an underestimated and potential role in the loosening process. In this article we will discuss the possible role of a bacterial biofilm and the importance of the local surrounding environment in "aseptic" loosening of hip prostheses.

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Clinical isolates of Staphylococcus aureus have osteolytic surface proteins and a proportion of the population have antibodies that block this activity: is this of prognostic significance?

Cited by 14 publications

References 0 publications

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Interaction of staphylococci with bone

The local inflammatory environment and microorganisms in “aseptic” loosening of hip prostheses

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