Clinical Investigation of the Efficacy of Chemomechanical Preparation with Rotary Nickel-Titanium Files for Removal of Endotoxin from Primarily Infected Root Canals

Martinho, Frederico Canato; Chiesa, Wanderson Miguel Maia; Marinho, Ariane Cássia Salustiano; Zaia, Alexandre Augusto; Ferraz, Caio Cézar Randi; Almeida, José Flávio Affonso de; Souza-Filho, Francisco José de; Gomes, Brenda Paula Figueiredo de Almeida

doi:10.1016/j.joen.2010.08.019

Cited by 63 publications

(115 citation statements)

References 29 publications

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“…24 The only techniques that have been tested so far and have showed a certain degree of effectiveness against LPS are (a) the use of calcium hydroxide as an intra-canal medication 12,25,26 as it hydrolyses lipid A and changes it into chains of fatty acid and nontoxic sugars, 27 and (b) the application of laser Nd:YAG. 9 The results obtained in this study reinforce the importance of instrumentation in addition to any disinfection protocol, 11,28,29 especially with regard to bacterial LPS that strongly adheres to the dentinal wall 6 and makes it necessary to use endodontic instruments for its removal. Some studies have reported endotoxin decrease up to 44.4%, 30 59.99%, 28 and 57.98% 10 after chemo-mechanical preparation.…”

Section: Discussionsupporting

confidence: 68%

“…Martinho et al 11 noticed 98.06% decrease in the endotoxin after performing canal preparation with NiTi (Mtwo ® ) rotatory tools under irrigation with 2.5% sodium hypochlorite solution, and 96.27% decrease when the rotatory preparation was associated with irrigation using apyrogenic saline solution. According to the authors, a basic factor that must be taken into account when performing chemo-mechanical preparation to decrease bacterial LPS is the enlargement the apical third.…”

Section: Discussionmentioning

confidence: 99%

“…2,11 The samples collected from the canals were mixed with the LAL reagent and automatically monitored over time with the help of a photometer until turbidity developed. The increase in optical density was measured by the reaction time, which is inversely proportional to the amount of LPS present in the sample.…”

Section: Determination Of Lps Levelsmentioning

confidence: 99%

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LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

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The present study aims to verify the effect of ozone gas (OZY ® System) and high frequency electric pulse (Endox ® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY ® System, one 120-second-pulse (OZY 1p); OZY ® System, four 24-second-pulses (OZY 4p); and Endox ® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

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LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

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“…A padronização da técnica reduziu a influência da instrumentação entre os grupos avaliados. A associação de hipoclorito com EDTA e da instrumentação rotatória também se mostrou eficiente em reduzir a quantidade de endotoxina após o término do preparo biomecânico (Martinho et al, 2010 No presente estudo, foram consideradas as porcentagens de redução volumétricas das lesões periapicais, após um ano do tratamento endodôntico. Embora Weiger et al, (2000) tenham sugerido que a avaliação do sucesso do tratamento deve ser realizada após quatro a cinco anos, a possibilidade de dropout e a dificuldade em acompanhar os pacientes têm feito com que os tratamentos sejam avaliados após períodos de aproximadamente um ano (Penesis et al, 2008;Patel et al, 2012a;Metska et al, 2013).…”

Section: Discussionunclassified

Tomografia computadorizada de feixe cônico como método de avaliação pós-tratamento endodôntico em sessão única ou em duas sessões

Maia¹

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, por sua dedicação à Endodontia, por sua contribuição à minha formação acadêmica e por ter realizado tanto pela nossa profissão.À Profa. Dra. Andiara De Rossi, por sua contribuição e observações na qualificação.À Profa. Dra. Raquel Assed Bezerra Segato, por sua contribuição na minha formação.Ao Prof. Dr. Idomeo Boneti Filho, por sua contribuição na minha formação acadêmica e profissional.À Profa. Dra. Angela Cristina Cilense Zuanon, por sua dedicação e motivação na minha formação profissional e pessoal.Ao Prof. Dr. Kleber Cortez Bonifácio, por sua amizade e incentivo para a realização do doutorado. AbstractIntroduction: Epidemiological studies have demonstrated a high prevalence of periapical lesion in different countries, stating that it is a health problem that affects great part of the world population. Aim: The aim of this randomized clinical study was to conduct a comparative evaluation after endodontic treatment of permanent teeth with chronic periapical lesion completed in a single session or in two sessions with 14-day calcium hydroxide interappointment intracanal medication, using cone beam computed tomography (CBCT). Methodology: Twenty-six pairs of teeth from 13 patients treated at the Endodontics Clinic of Ceuma University (UniCeuma), São Luís, MA, Brazil, were selected and randomly assigned to two groups (n=13/group) by simple random sampling (draw). In Group I, the teeth were instrumented and filled in the same session, while in Group II, after instrumentation, an intracanal medication (Calen ® paste) was used for 14 days between

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“…A grande redução na quantidade de micro-organismos após o preparo químico-mecânico foi investigada com resultados significativos, ratificando a importância desta etapa (MARTINHO et al, 2010).…”

Section: Revisão De Literaturaunclassified

Descontaminação intra-tubular de dentes bovinos por pastas de hidróxido de calcio associadas a diferentes veículos

Pereira¹

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Autorizo, exclusivamente para fins acadêmicos e científicos, a reprodução total ou parcial desta dissertação/tese, por processos fotocopiadores e outros meios eletrônicos. Assinatura:Data: DEDICATÓRIA A Deus, pelo dom da vida, que é a maior viagem de todas, obrigada por guiar minha trajetória e iluminar meu caminho, e sempre estar do meu lado! Aos meus pais, Silvia e Arlindo, sem os quais eu não seria capaz de fazer e conquistar nada em minha vida. Obrigada por sempre apoiarem meus sonhos e planos, pela compreensão, paciência e reconhecimento das minhas vitórias. foi possível observar a melhor ação antimicrobiana contra E. faecalis pela pasta de hidróxido de cálcio com propilenoglicol e PMCC nas regiões superficial e profunda da massa dentinária.Palavras-chave: Enterococcus faecalis, hidróxido de cálcio, microscopia.ABSTRACT Intra-tubular desinfection of bovine teeth contamined with calcium hydroxide pastes associated with different vehicles.Biomechanical preparation followed by irrigation in endodontic treatment of infected root canals has been shown, however, some micro-organisms still survive. The most frequently founded bacterial specie in endodontic treatment fail is Enterococcus faecalis. To combat it, different intra-canal medications are used, mainly the calcium hydroxide pastes and other substances have been associated to calcium hydroxide to leverage it antimicrobial effect. Thus, it's relevant to compare in vitro the antimicrobial ability of calcium hydroxide with different vehicles and additives, againstEnterococcus faecalis, through microbiological culture and confocal laser scanning microscopy (CLSM). For this, standard dentine tubes were sterilized and then infected with the bacteria for 5 days and were divided in five test-groups, submitted to medication for 15 days with calcium hydroxide paste in distilled water (G1), in propylene glycol (G2), besides clacium hydroxide in propylene glycol with ethanol extract of propolis (G3), chlorexidine (G4) and CPMC (G5). After the medication period, the bacterial collection of the dentine´s fragments was done with Largo's drill in a sterilized device, to count micro-organisms still viable in colony forming units.The CLSM evaluated the viable (green) and dead (red) bacteria inside the dentinal tubules with the aid of the Live and Dead® dye. There was dentinal intra-tubular antimicrobial action against Enterococcus faecalis for all the tested calcium hydroxide pastes. From this work was possible to observe a better antimicrobial activity againstEnterococcus faecalis by the calcium hydroxide paste in propylene glycol and CPMC in superficial and deep areas of the dentine.

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Clinical Investigation of the Efficacy of Chemomechanical Preparation with Rotary Nickel-Titanium Files for Removal of Endotoxin from Primarily Infected Root Canals

Cited by 63 publications

References 29 publications

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

Tomografia computadorizada de feixe cônico como método de avaliação pós-tratamento endodôntico em sessão única ou em duas sessões

Descontaminação intra-tubular de dentes bovinos por pastas de hidróxido de calcio associadas a diferentes veículos

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