Clinical importance of fibulin-5 immunohistochemical staining in breast lesions

Abstract

The aim of this study was to evaluate the clinical importance of fibulin-5, which has been shown to display tumour-promoting and tumour-protective functions in breast lesions. Sixty-two breast cancer patients, 19 patients with fibroadenoma, and 15 healthy breast tissues were enrolled. Forty-seven patients had invasive ductal carcinoma (IDC) (12 of them also had in situ carcinoma DCIS), and 15 had invasive lobular carcinoma (ILC). A scoring system from 0 to 4 was used to evaluate the fibulin-5 staining according to the percentage of stained cells. The median values of fibulin-5 staining scores of the breast cancer, fibroadenoma, and healthy breast tissues were 2 (range: 0-4), 3 (range: 3-4), and 4 (range: 1-4), respectively, and the difference is statistically significant (p = 0.0001). There was no significant difference between the fibulin-5 scores of IDC, ILC, and DCIS. Fifteen patients with triple-negative breast cancer (TNBC) had the lowest fibulin-5 score (p < 0.0001). The median fibulin-5 scores of the patients according to Ki-67 index ≥ 14% and < 14% were 2 (range: 1-5) and 4 (range: 1-5), respectively, and the difference is statistically significant (p = 0.001). These data can be explained by the inhibitory effect of fibulin-5 on epithelial cell proliferation, which is closely related to differentiation and prognosis.

The Pathogenic Effects of Fusobacterium nucleatum on the Proliferation, Osteogenic Differentiation, and Transcriptome of Osteoblasts

“…The upregulation of Tnfrsf1b, which belongs to the TNF receptor superfamily, has also been found in gastric cancer tissues ( Rossi et al, 2019 ), and Tnfrsf1b can promote colorectal carcinogenesis ( Liu et al, 2018 ). Fbln5 can inhibit epithelial cell proliferation and carries out tumor-promoting and tumor-protective functions in breast lesions ( Karanis et al, 2019 ). We speculate that long-term direct stimulation with F. nucleatum and the inflammatory environment lead to changes in the intracellular environment and intracellular signaling pathways, which may play a role in the development of osteosarcoma; this might be another reason for the reduced osteogenic ability of osteoblasts.…”