Clinical Implications of Marker Expression of Carcinoma-Associated Fibroblasts (CAFs) in Patients with Epithelial Ovarian Carcinoma After Treatment with Neoadjuvant Chemotherapy

Mhawech‐Fauceglia, Paulette; Wang, Dan; Samrao, Damanzoopinder; Kim, Grace; Lawrenson, Kate; Meneses, Teodulo; Liu, Song; Yessaian, Annie A.; Pejović, Tanja

doi:10.1007/s12307-013-0140-4

Cited by 29 publications

(39 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, none of these genes showed signi cant association with HSOC survival. This result may be partly due to negative expression of αSMA (30).…”

Section: Discussionmentioning

confidence: 99%

High expression of Fibroblast activation protein (FAP) predicts poor outcome in high-grade serous ovarian cancer

Xue

Rong

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: High-grade serous ovarian cancer (HSOC) is a fatal form of ovarian cancer. Previous studies indicated some potential biomarkers for clinical evaluation of HSOC prognosis. However, there is a lack of systematic analysis of different expression genes (DEGs) to screen and detect significant biomarkers of HSOC. Method and materials: TCGA database was conducted to analyze relevant genes expression in HSOC. Outcomes of candidate genes expression, including overall survival (OS) and progression-free survival (PFS), were calculated by Cox regression analysis for hazard rates (HR). Histopathological investigation of the identified genes was carried out in 151 Chinese HSOC patients to validate gene expression in different stages of HSOC. Results: Of all 57,331 genes that were analyzed, FAP was identified as the only novel gene that significantly contributed to both OS and PFS of HSOC. In addition, FAP had a consistent expression profile between carcinoma-paracarcinoma and early-advanced stages of HSOC. Immunological tests in paraffin section also confirmed that up-regulation of FAP was present in advanced stage HSOC patients. Prediction of FAP network association suggested that FN1 could be a potential downstream gene which further influenced HSOC survival. Conclusions: High-level expression of FAP was associated with poor prognosis of HSOC via FN1 pathway.

show abstract

“…Nevertheless, none of these genes showed signi cant association with HSOC survival. This result may be partly due to negative expression of αSMA (30).…”

Section: Discussionmentioning

confidence: 99%

High expression of Fibroblast activation protein (FAP) predicts poor outcome in high-grade serous ovarian cancer

Xue

Rong

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Fibroblast cells acquire phenotypes that are perpetually activated, and their identification is made by the expression of smooth muscle alpha-actin and alpha-activating protein of fibroblasts, which are called CAFs [18]. …”

Section: Discussionmentioning

confidence: 99%

“…The fibroblasts associated with cancer manifest significance in tumor progression, structural and functional support in the tumor microenvironment. The population of CAFs is vast and is composed of several markers including the alpha-actin and smooth muscle alpha-actin activation protein [6, 18]. CAFs are responsible for the activation of fibroblasts that have biological significance in lymphangiogenesis, angiogenesis, tumorigenesis, cell degradation, extracellular matrix production and tumor invasion, and metastatic processes.…”

Section: Discussionmentioning

confidence: 99%

“…CAFs are responsible for the activation of fibroblasts that have biological significance in lymphangiogenesis, angiogenesis, tumorigenesis, cell degradation, extracellular matrix production and tumor invasion, and metastatic processes. In addition, they participate in the production of cytokines, chemokines, growth factors, and mainly in immunomodulatory processes [6, 18]. …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Role of Alpha-Smooth Muscle Actin and Fibroblast Activation Protein Alpha in Ovarian Neoplasms

Silva¹,

Jammal²,

Etchebehere

et al. 2018

Gynecol Obstet Invest

View full text Add to dashboard Cite

Background/Aims: Studies show that tumor growth is not just determined by the presence of malignant cells, since interactions between cancer cells and stromal microenvironment have important impacts on the cancer growth and progression. Cancer-associated fibroblasts play a prominent role in this process. The aims of the study were to investigate 2 cancer-associated fibroblasts markers, alpha-smooth muscle actin (α-SMA), and fibroblast activation protein alpha (FAP) in the stromal microenvironment of benign and malignant ovarian epithelial neoplasms, and to relate their tissue expression with prognostic factors in ovarian cancer. Methods: α-SMA and FAP were evaluated by immunohistochemistry in malignant (n = 28) and benign (n = 28) ovarian neoplasms. Fisher’s exact test was used with a significance level lower than 0.05. Results: FAP immunostaining was stronger in ovarian cancer when compared to benign neoplasms (p = 0.0366). There was no significant difference in relation to α-SMA expression between malignant and benign ovarian neoplasms as well as prognostic factors. In ovarian cancer, FAP stainings 2/3 was significantly related to histological grades 2 and 3 (p = 0.0183). Conclusion: FAP immunostaining is more intense in malignant neoplasms than in benign ovarian neoplasms, as well as in moderately differentiated and undifferentiated ovarian carcinomas compared to well-differentiated neoplasms, thus indicating that it can be used as a marker of worse prognosis.

show abstract

“…The correlations between claudins (CLDN6 and CLDN10) expression and various immune cells in ltration of ovarian cancer were shown in Table 2. As shown in Relevant evidences reported that cancer associated broblast (CAF) plays an important role in the progression of ovarian cancer [24,25] . Interestingly, we also found that CAF has a positive correlation with CLDN6 expression, but a negative correlation with CLDN10 expression.…”

Section: Correlation Analysis Between Claudins and The Tumor Microenvmentioning

confidence: 94%

CLDN6 and CLDN10 are Associated with Immune Infiltration of Ovarian Cancer: A Study of Claudin Family

Gao

Peng

Cao

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Claudin family is a group of membrane proteins related to tight junction. There are many studies about them in cancer, but few studies pay attention to the relationship between them and the tumor microenvironment. In our research, we mainly focused on the genes related to the prognosis of ovarian cancer, and explored the relationship between them and the tumor microenvironment of ovarian cancer.Methods: The cBioProtal provided the genetic variation pattern of claudin gene family in ovarian cancer. The ONCOMINE database and Gene Expression Profiling Interactive Analysis (GEPIA) were used to exploring the mRNA expression of claudins in cancers. The prognostic potential of these genes was examined via Kaplan-Meier plotter. Immunologic signatures were enriched by gene set enrichment analysis (GSEA). The correlations between claudins and the tumor microenvironment of ovarian cancer were investigated via Tumor Immune Estimation Resource (TIMER).Results: In our research, claudin genes were altered in 363 (62%) of queried patients/samples. Abnormal expression levels of claudins were observed in various cancers. Among them, we found that CLDN3, CLDN4, CLDN6, CLDN10, CLDN15 and CLDN16 were significantly correlated with overall survival of patients with ovarian cancer. GSEA revealed that CLDN6 and CLDN10 were significantly enriched in immunologic signatures about B cell, CD4 T cell and CD8 T cell. What makes more sense is that CLDN6 and CLDN10 were found related to the tumor microenvironment. CLDN6 expression was negatively correlated with immune infiltration level in ovarian cancer, and CLDN10 expression was positively correlated with immune infiltration level in ovarian cancer. Further study revealed the CLDN6 expression level was negatively correlated with gene markers of various immune cells in ovarian cancer. And, the expression of CLDN10 was positive correlated with gene markers of immune cells in ovarian cancer.Conclusions: CLDN6 and CLDN10 were prognostic biomarkers, and correlated with immune infiltration in ovarian cancer. Our results revealed new roles for CLDN6 and CLDN10, and they were potential therapeutic targets in the treatment of ovarian cancer.

show abstract

Clinical Implications of Marker Expression of Carcinoma-Associated Fibroblasts (CAFs) in Patients with Epithelial Ovarian Carcinoma After Treatment with Neoadjuvant Chemotherapy

Cited by 29 publications

References 29 publications

High expression of Fibroblast activation protein (FAP) predicts poor outcome in high-grade serous ovarian cancer

High expression of Fibroblast activation protein (FAP) predicts poor outcome in high-grade serous ovarian cancer

Role of Alpha-Smooth Muscle Actin and Fibroblast Activation Protein Alpha in Ovarian Neoplasms

CLDN6 and CLDN10 are Associated with Immune Infiltration of Ovarian Cancer: A Study of Claudin Family

Contact Info

Product

Resources

About