Clinical Implication of Immunohaematological Tests in <scp>ABO</scp> haemolytic disease of newborn: Revisiting an old disease

Das, Soumya; Shastry, Shamee; Pentapati, Kalyana Chakravarthy; Baliga, Poornima

doi:10.1111/tme.12718

Cited by 20 publications

(15 citation statements)

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“…Others have revisited ABO‐HDFN in order to assess the frequency of the disease and at the same time identify predictive parameters [18, 19]. They both found the direct antiglobulin testing of the newborns more informative than the maternal anti‐A/B IgG titre [18, 19]. Our approach with IgG subclass determination of maternal anti‐A/B seems to point in a new direction for predicting haemolysis in the newborn.…”

Section: Discussionmentioning

confidence: 97%

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ABO haemolytic disease of the newborn: Improved prediction by novel integration of causative and protective factors in newborn and mother

et al. 2021

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Background and Objectives Prediction of haemolytic disease of the foetus and newborn (HDFN) caused by maternal anti‐A/‐B enables timely therapy, thereby preventing the development of kernicterus spectrum disorder. However, previous efforts to establish accurate prediction methods have been only modestly successful. Materials and Methods In a case–control study, we examined 76 samples from mothers and 76 samples from their newborns; 38 with and 38 without haemolysis. The IgG subclass profile of maternal anti‐A and anti‐B was determined by flow cytometry. Samples from newborns were genetically analysed for the A2 subgroup, secretor and FcγRIIa receptor alleles. Results Surprisingly, we found a correlation between the newborn secretor allele and haemolysis (p = 0.034). No correlation was found for FcγRIIa alleles. The A2 subgroup was found only in newborns without haemolysis. Unexpectedly, different reaction patterns were found for maternal anti‐A and anti‐B; consequently, the results were treated separately. For the prediction of haemolysis in A‐newborns, the maternal IgG1 subclass determination resulted in an accuracy of 83% at birth. For B‐newborns, an accuracy of 91% was achieved by the maternal IgG2 subclass determination. Conclusion We improved the prediction of ABO‐HDFN by characterizing maternal anti‐A and anti‐B by flow cytometry and we presented genetic traits in newborns with correlation to haemolysis. We propose a new understanding of A‐ and B‐substances as immunogens that enhance the maternal immune response and protect the newborn, and we suggest that the development of ABO‐HDFN is different when caused by maternal anti‐A compared to maternal anti‐B.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

ABO haemolytic disease of the newborn: Improved prediction by novel integration of causative and protective factors in newborn and mother

et al. 2021

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Section: Introductionmentioning

confidence: 99%

“…And neonatal jaundice induced blood incompatibility [4]. Some studies have shown the correlation of neonatal hyperbilirubinemia include G6PD de ciency, serum vitamin D level, ASO haemolytic disease [4][5][6]. Some causes of signi cant hyperbilirubinemia were relation with erythrocyte membrane defects, red blood cell enzyme de ciencies [7,8].…”

Section: Introductionmentioning

confidence: 99%

The Coagulation Analysis of Reveals Novel Biomarkers for Neonatal Hyperbilirubinemia in Full Term Newborn

Zhou

Liu

2021

Preprint

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Background: The coagulation test is used to evaluate in various diseases. Little is known about correlation between the coagulation test and neonatal hyperbilirubinemia. The aim of the study was to determine the neonatal coagulation analysis value in infants with neonatal hyperbilirubinemia as reliable markers. Methods: All newborns were born and inpatients in the newborn wards of the Chengdu Second People’s Hospital. Measurement of serum bilirubin was done to diagnose hyperbilirubinemia. All neonates involved were full term of either gender without any significant illness or major congenital malformations. Data were collected from newborns who had neonatal hyperbilirubinemia and healthy in Chengdu Second People’s Hospital. Prothrombin time(PT), Thrombin time(TT), Fibrinogen(Fbg), activated partial thromboplastin time(APTT), and calculated international normalized ration(INR) values were recorded. Linear relationship between INR and level of total bilirubin and indirect bilirubin were analyzed by linear regression. The receiver operating curve(ROC) and the area under the curve(AUC) were made evaluated. Results: In this case-control study, the mean PT, APTT level were significant higher in the infants with hyperbilirubinemia group compared to the healthy infants. We found the positive correlation between INR and total bilirubin(R=0.3327; P<0.0001). Furthermore, there was also significant positive correlation between INR and indirect bilirubin(R=0.3402; P<0.0001). Meanwhile, INR in neonates with hyperbilirubinemia significantly achieved an AUC of 0.800(95% CI=0.6288-0.9712, cutoff value 1.060, specificity71.43%, and sensitivity 80.00%).Conclusions: PT, APTT were significant high in neonatal hyperbilirubinemia group. Correlation were observed between the INR and neonatal hyperbilirubinemia. Importantly, the study revealed INR as novel biomarkers for diagnosis of neonatal hyperbilirubinemia in full term newborn.

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Section: Introductionmentioning

confidence: 99%

Correlation between the coagulation test and neonatal hyperbilirubinemia

Zhou¹,

Li²,

Fu³

2020

Preprint

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Background The coagulation test is used to diagnose the hematological diseases. Little is known about correlation between the coagulation test and neonatal hyperbilirubinemia. The aim of the study was to describe neonatal coagulation test in infants with neonatal hyperbilirubinemia. Methods Data were collected from newborns who had neonatal hyperbilirubinemia and healthy in Chengdu Second People’s Hospital. Prothrombin time(PT), Thrombin time(TT), Fibrinogen(Fbg), activated partial thromboplastin time(APTT), and calculated international normalized ration(INR) values were recorded. Linear relationship between INR and level of total bilirubin and indirect bilirubin were analysed by linear regression. Comparisons of two groups were made by GraphPad Prism 8. Results In this case-control study, the mean PT, APTT level were significant higher in the infants with hyperbilirubinemia group compared to the healthy infants. We found the correlation between INR and total bilirubin(R = 0.3327; P < 0.0001). There was also correlation between INR and indirect bilirubin(R = 0.3402; P < 0.0001). Conclusions PT, APTT were significant high in neonatal hyperbilirubinemia group. Correlation were observed between the coagulation test and neonatal hyperbilirubinemia.

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Clinical Implication of Immunohaematological Tests in ABO haemolytic disease of newborn: Revisiting an old disease

Cited by 20 publications

References 20 publications

ABO haemolytic disease of the newborn: Improved prediction by novel integration of causative and protective factors in newborn and mother

ABO haemolytic disease of the newborn: Improved prediction by novel integration of causative and protective factors in newborn and mother

The Coagulation Analysis of Reveals Novel Biomarkers for Neonatal Hyperbilirubinemia in Full Term Newborn

Correlation between the coagulation test and neonatal hyperbilirubinemia

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