Clinical impact and prevalence of MRSA CC398 and differences between MRSA-TetR and MRSA-TetS in an area of Spain with a high density of pig farming: a prospective cohort study

Reynaga, Esteban; Torres, Carmén; García-Núñez, Marian; Navarro, Marian; Vilamala, Anna; Puigoriol, Emma; Lucchetti, Gianni; Sabrià, Miquel

doi:10.1016/j.cmi.2017.03.019

Cited by 12 publications

(6 citation statements)

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“…However, this genetic lineage has been detected in humans living in an area with high pig farming density in this country (Reynaga et al . 2017). The MRSA CC9 isolate detected in our study did not exhibit a multidrug resistance phenotype nor tetracycline resistance, which is contrary to previous findings (Lo et al .…”

Section: Discussionmentioning

confidence: 99%

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

et al. 2021

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Aims: To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018-2019. Methods and Results: The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular techniques. The transferability of the cfr gene was assessed by conjugation and its genetic environment was determined by PCR mapping. In all, 21 isolates were methicillin-resistant S. aureus (MRSA) carrying the mecA gene (SCCmecV or non-typeable SCCmec), whereas the remaining two were methicillin-susceptible (MSSA). All but one MRSA isolates (n = 20) belonged to the CC398, being the spa t011 the most prevalent (n = 11). The remaining MRSA and the two MSSA isolates were ascribed to ST9/CC9. The S. aureus isolates exhibited resistance to (number of resistant isolates): b-lactamics (21), erythromycin and/or clindamycin (20), aminoglycosides (7), tetracycline (22), fluoroquinolones (14), chloramphenicol (5) and linezolid (1). The S. aureus isolates did not carry any of the virulence genes studied. One MRSA belonging to the CC398 showed linezolid resistance mediated by the cfr gene. The cfr gene was co-located with fexA in the Tn558 variant previously reported in the S. aureus plasmid pSCFS7. Conclusions: Two major livestock-associated genetic lineages were detected among pigs with signs of infection in Spain. The presence of the cfr gene among LA-MRSA-CC398 is of great concern not only for veterinary medicine, but also for humans in close contact. Significance and Impact of the Study: This work describes the molecular characterization of S. aureus isolates recovered from pigs with signs of infection and we report, as far as we know, the first description of MRSA-CC9 from pigs in Spain. Moreover, the detection of a MRSA-CC398 isolate carrying the multiresistance cfr gene highlights the need for continuous surveillance and awareness of LA-MRSA.

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Section: Discussionmentioning

confidence: 99%

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

et al. 2021

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“…After confirmation of the mecA gene, it is important to submit the strains to additional molecular characterization. The techniques that are generally used are as follows: staphylococcal protein A (spa) typing [59,61,[66][67][68], multilocus sequence typing (MLST) [59,61,64,66,67,70], pulsed field gel electrophoresis (PFGE) [64][65][66], Staphylococcal cassette chromosome mec (SCCmec) typing [59,66].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

2019

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Foodborne diseases (FBD) occur worldwide and affect a large part of the population, being a cause of international concern among health authorities. Staphylococcus aureus can be transmitted by contaminated food, and it is one of the pathogens that most cause foodborne outbreaks in Brazil. Currently, this organism's ability in developing resistance to antibiotics is notorious; methicillin-resistant Staphylococcus aureus-MRSA-is known for its resistance to methicillin, oxacillin, and others. MRSA is one of the leading causes of infections, becoming a major threat to human health worldwide due to the numerous toxins that can produce. At first, the transmission of MRSA occurred in clinical environments; but in recent decades, its presence has been reported in the community, outside the hospital environment, including food and food-producing animals around the world. In this review, information about MRSA was gathered to verify MRSA incidence in the world but especially in Brazil in food samples, food handlers, food-producing animals, and food processing environments. The studies show that MRSA is easily found and in certain cases with high frequency, thus representing a potential risk to public health.

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“…The county of Osona has one of the highest pig densities in Spain 5 . In a recent study in this same area of Osona, 57.8% of pig-farm workers were carriers of MRSA-ST398 5 , and approximately 45% of patients diagnosed with MRSA in the hospital of this county in 2015 had the ST398 lineage 10 . These data suggest that the MRSA-ST398 clone plays a significant role both in colonization and in infections in the population of the Osona region.…”

Section: Discussionmentioning

confidence: 92%

Prevalence of MRSA ST398 Carriage in Nursing Home Residents in an Area of Spain With a High Density of Pig Farming

Reynaga

Torres

García-Núñez

et al. 2017

Infect. Control Hosp. Epidemiol.

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Clinical impact and prevalence of MRSA CC398 and differences between MRSA-TetR and MRSA-TetS in an area of Spain with a high density of pig farming: a prospective cohort study

Cited by 12 publications

References 11 publications

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

Prevalence of MRSA ST398 Carriage in Nursing Home Residents in an Area of Spain With a High Density of Pig Farming

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