Clinical hematological and biochemical parameters in Swiss, BALB/c, C57BL/6 and B6D2F1 Mus musculus

Abstract

BackgroundAnimal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these laboratory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice.MethodsWe evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the systems pocH‐100iV Diff™ for 19 hematological parameters and VITROS® 350 for 12 biochemical parameters.ResultsResults are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability.ConclusionThus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.

Development of a Quantitative FMRP Assay for Mouse Tissue Applications

“…Considering a physiological reduction in WBC number occurring between 3 weeks and 7-10 weeks of life, it is possible that some of the FMRP reduction may be accounted for by the WBC number difference. However, the hematological reference values for C57BL/6 mice at various ages are not uniform [46][47][48] and require a more detailed assessment for the normalization of FMRP on an individual basis. The study was limited by the volume of the collected blood in the youngest group.…”

Targeting Serotonin With Common Antidepressants Induces Rapid Recovery From Cytopenia

“…( A ), ( B ), ( D ): Horizontal lines represent lower limit of normal range for hemoglobin (11 g/dL), platelets (300 × 10 3 /mm 3 ), and neutrophils (0.5 × 10 3 /mm 3 ), and threshold for profound anemia (8 g/dL), in mice. 7 , 8 (A) In vivo rescue experiments with fluoxetine: hemoglobin (g/dL), platelet (10 3 /mm 3 ), and neutrophil (10 3 /mm 3 ) levels in WT mice ( n = 7) treated with fluoxetine or placebo (control) from D − 1 (ie, 1 day before sublethal irradiation) until end of experiment; data from 3 independent experiments. ( B ) In vivo rescue experiments with G-CSF, alone or with fluoxetine: hemoglobin (g/dL), platelet (10 3 /mm 3 ), and neutrophil (10 3 /mm 3 ) levels in WT mice ( n = 5) treated with G-CSF, alone or with fluoxetine, from D − 1 before sublethal irradiation until end of experiment; data from 3 independent experiments.…”

“…We compared established murine biological parameters in mice treated with 20 mg/kg/day fluoxetine (Prozac ) administered in drinking water, starting D −1 (ie, day before irradiation) unless stated otherwise, to those in control mice—with or without granulocyte colony-stimulating factor (G-CSF, filgrastim). 7 , 8 G-CSF was administered intraperitoneally at 5 µg/kg/day from D5 to the end of the experiment.…”

In Vitro and In Vivo Studies for the Investigation of γ-Globin Gene Induction by Adhatoda vasica: A Pre-Clinical Study of HbF Inducers for β-Thalassemia

“…In another set of experiments, C57BL/6 mice were orally administrated with an aqueous extract of A. vasica at various doses (100, 200, and 500 mg/kg). Post-treatment, mice were sacrificed, and blood samples were collected analyzed for hematological and serum biochemical indices as described elsewhere ( Silva-Santana et al, 2020 ).…”