Clinical guidelines for cryoprecipitate transfusions

Галстян, Г. М.; Гапонова, Т. В.; Жибурт, Е Б; Balashova, E.N.; Берковский, А. Л.; Анатольевна, Быстрых Оксана; Купряшов, А.А.; Оловникова, Н. И.; Ошоров, А В; Рыбка, М.М.; Троицкая, В В; Буланов, А. Ю.; Zhuravel, S. V.; Лубнин, А. Ю.; Мазурок, В.А.; Недомолкин, С. В.; Певцов, Д. Э.; Рогачевский, О. В.; Салимов, Э. Л.; Trakhtman, Pavel; Чжао, А. В.; Sherstnev, F. S.; Савченко, В Г

doi:10.35754/0234-5730-2020-65-1-87-114

Cited by 8 publications

(1 citation statement)

References 110 publications

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“…Высокоточное определение гемостатических показателей плазмы и продуктов плазмы крови значимо как для производственной трансфузиологии [1][2][3], так и для контроля эффективности клинического применения этих продуктов [4]. Существуют актуальные статистические проблемы анализа таких данных и ме-тодологические проблемы получения сопоставимых результатов независимых исследований [5,6].…”

Section: Introductionunclassified

Effect of increasing the number of repeated measurements on the accuracy of determining factor VIII activity and fibrinogen concentrations in blood plasma

Lemondzhava,

Sidorkevich,

Kasyanov

2024

Gematologiâ i transfuziologiâ

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Introduction. Highly accurate determination of hemostatic indices of plasma and blood plasma products is important for industrial transfusiology and monitoring the effi cacy of their clinical application. Repeated measurements increase statistical power, thereby reducing the likelihood of committing a second-order error, which is described as a false negative result and occurs when a test fails to detect a truly existing effect. Aim: to evaluate the effect of increasing the number of repeated measurements on the accuracy of factor VIII activity and fi brinogen concentrations in donor plasma. Materials and methods. Human donor plasma used in the study was obtained by centrifugation of whole blood. The criterion for inclusion of biomaterial in the study was the presence of a non-repeatable combination of donor characteristics: sex, age, blood group and Rhesus affi liation by the presence of D antigen. Whole blood donors for this work were male and female aged between 38 and 53 years with groups: O(I), A(II) and B(III). 27 repeated measurements of factor VIII activity by the one-stage clotting method and fi brinogen concentrations by the Clauss clotting method were performed on automatic coagulometer ACL TOP 300 with HemosIL reagents. Results. For factor VIII activity, the difference in values recorded in repeated measurements reached 20 IU/100 ml, and for fi brinogen concentrations the maximum difference was 0.29 g/L. The calculation of the change in the size of the confi dence interval with increasing number of repeated measurements is presented. While the decrease in size from the second to the fourth repeated measurement averaged 83.5 % for the measurement of factor VIII activity and 61.7 % for fi brinogen concentrations, from the fi fth to the seventh it was 16.9 % and 21.5 %, respectively. Conclusions. Despite the pre-analytical measures taken to reduce random error, blood plasma parameters of the same donation can take values in a wide range. Increasing the number of repeat measurements from one to three in the case of measuring factor VIII activity and fi brinogen concentrations is an effective means of improving the accuracy of these indices. However, with subsequent repeated measurements there will be a decrease in statistical power growth.

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Section: Introductionunclassified

Effect of increasing the number of repeated measurements on the accuracy of determining factor VIII activity and fibrinogen concentrations in blood plasma

Lemondzhava,

Sidorkevich,

Kasyanov

2024

Gematologiâ i transfuziologiâ

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Evaluation of the applicability of a chromogenic assay of changes in antithrombin III activity to monitor the accuracy of thermal effects on blood plasma in thawing and warming systems

Lemondzhava,

Sidorkevich,

Kasyanov

2024

Biomed Eng

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Biological characteristics of the enteroaggregative <i>escherichia coli </i>ont:h30 18-726 (no. B-8857) strain isolated from a patient with ulcerative colitis and a new method of pcr identification

Makarova,

Kruglov,

Kaftyreva

2023

Russian Journal of Infection and Immunity

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Abstract. The aim of the work is to study the biological properties of the enteroaggregative Escherichia coli strain isolated from the biopsy material of the colon mucosa of a patient with ulcerative colitis, and to develop a new method for PCR identification of E. coli strains. Methods: The study involved 46 patients with a verified diagnosis of ulcerative colitis. Isolation of 87 strains of Escherichia coli was carried out from colon biopsy material obtained during a standard endoscopic procedure. The description of the biochemical properties of bacteria was combined with the molecular genetic determination of virulence determinants and the presence of antibiotic resistance mechanisms. Using the AmpliSense Escherichiose - FL reagent kit, a screening for the presence of diarrheagenic E. coli strains was performed, which revealed the presence of one enteroaggregative strain of E. coli (EAgEC). The methodological approach to creating a new method for strain identification was based on the search for a unique genetic sequence in the glutamate decarboxylase (gad) gene. Results: E. coli 18-726 can be described biochemically as typical of its species. The studied strain was characterized by a multiple resistance phenotype (MDR) to antibacterial chemotherapy drugs, as well as a lack of sensitivity to five bacteriophage preparations. The E. coli 18-726 strain had a unique sequence of the gene encoding the O-antigen, which differed from 188 known O-antigens (ONT) and, by the identity of the nucleotide sequence of the gene encoding the synthesis of the H-antigen, the strain belonged to the H30 serovar. Thus, the antigenic formula of strain EAgEC 18-726 was expressed as ONT:H30. The E. coli 18-726 strain contained a significant spectrum of genes that implement the properties of virulence (iss, capU, aggA, aggB, aggC, aggD, aap, aar) and resistance to antibacterial drugs (blaCTX-M-15, blaTEM-1B, aadA1, aadA5, mph(A), catB3). A new method for identifying enteroaggregative E. coli in chronic bowel disease is based on the polymerase chain reaction method using primers for a specific region of the glutamate decarboxylase (gad) gene. Conclusion: The biochemical, antigenic and molecular genetic properties of Escherichia coli ONT:H30 18-726 strain (No. B-8857) isolated from a patient with histomorphologically diagnosed ulcerative colitis were analyzed. 2. A method for PCR identification of enteroaggregative strains of Escherichia coli based on the detection of a specific region of the glutamate decarboxylase gene in the genome of strains has been created and patented.

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Clinical guidelines for cryoprecipitate transfusions

Cited by 8 publications

References 110 publications

Effect of increasing the number of repeated measurements on the accuracy of determining factor VIII activity and fibrinogen concentrations in blood plasma

Effect of increasing the number of repeated measurements on the accuracy of determining factor VIII activity and fibrinogen concentrations in blood plasma

Evaluation of the applicability of a chromogenic assay of changes in antithrombin III activity to monitor the accuracy of thermal effects on blood plasma in thawing and warming systems

Biological characteristics of the enteroaggregative <i>escherichia coli </i>ont:h30 18-726 (no. B-8857) strain isolated from a patient with ulcerative colitis and a new method of pcr identification

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