Clinical gamasoidosis and antibody response in two patients infested with Ornithonyssus bursa (Acari: Gamasida: Macronyssidae)

Lima-Barbero, José Francisco; Sánchez‐Sánchez, Marta; Cabezas-Cruz, Alejandro; Mateos-Hernández, Lourdes; Contreras, Marinela; Mera, Isabel G. Fernández de; Villar, Margarita; Fuente, José de la

doi:10.1007/s10493-019-00408-x

Cited by 15 publications

(17 citation statements)

References 42 publications

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“…Antibodies from egg yolk were extracted as previously described [27] and stored at −20 °C. The IgY antibody levels against Deg-AKR and a mite-soluble protein extract obtained as previously described [28] were determined by ELISA in sera and egg yolks. High absorption-capacity 96-well plates (Immunoplate, SPL Life Sciences, Syeonggi-do, Korea) were coated overnight at 4 °C with 0.25 µg/well of each antigen.…”

Section: Methodsmentioning

confidence: 99%

Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin

et al. 2019

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The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae (Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of its efficacy as a vaccine candidate for the control of PRM. The anti-Deg-AKR serum IgY antibodies in hen sera and egg yolk were higher in vaccinated than control animals throughout the experiment. The results demonstrated the efficacy of the vaccination with Deg-AKR for the control of PRM by reducing mite oviposition by 42% following feeding on vaccinated hens. A negative correlation between the levels of serum anti-Deg-AKR IgY and mite oviposition was obtained. These results support Deg-AKR as a candidate protective antigen for the control of PRM population growth.

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Section: Methodsmentioning

confidence: 99%

Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin

et al. 2019

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“…After three cycles of sonication vortex, the homogenate was centrifuged at 200 g for 5 min at 4 • C, and the supernatant was quantified using an RC DC protein assay (BioRad, Hercules, CA, USA) with BSA as standard. This methodology has been previously shown to preserve the presence of the α-Gal epitope in extracted proteins (Lima-Barbero et al, 2019).…”

Section: Protein Extracts From Zebrafish Tissues and Feeds Human Hl6mentioning

confidence: 99%

Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Contreras

Pacheco

Alberdi

et al. 2020

Front. Cell. Infect. Microbiol.

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Ticks are arthropod ectoparasite vectors of pathogens and the cause of allergic reactions affecting human health worldwide. In humans, tick bites can induce high levels of immunoglobulin E antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate anaphylactic reactions known as the alpha-Gal syndrome (AGS) or red meat allergy. In this study, a new animal model was developed using zebrafish for the study of allergic reactions and the immune mechanisms in response to tick salivary biogenic substances and red meat consumption. The results showed allergic hemorrhagic anaphylactic-type reactions and abnormal behavior patterns likely in response to tick salivary toxic and anticoagulant biogenic compounds different from α-Gal. However, the results showed that only zebrafish previously exposed to tick saliva developed allergic reactions to red meat consumption with rapid desensitization and tolerance. These allergic reactions were associated with tissue-specific Toll-like receptor-mediated responses in types 1 and 2 T helper cells (T H 1 and T H 2) with a possible role for basophils in response to tick saliva. These results support previously proposed immune mechanisms triggering the AGS and provided evidence for new mechanisms also potentially involved in the AGS. These results support the use of the zebrafish animal model for the study of the AGS and other tick-borne allergies.

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“…In both cases the source of mites was traced to abandoned bird nests. Recently, isolated urban cases of O. bursa infestation were reported in an Italian 70years-old male (Sicily, Palermo) (42) and in a couple (76-yearsold, male and 70-years-old, female) in Spain (Girona) (43); the cause of the infestation was attributed to presence of backyard chickens and dove nests in the garden, respectively. Although in both cases mites were only collected on patients and not on birds, the papular dermatitis disappeared after removal of chickens and dove nests, confirming this as the probable source of infestation.…”

Section: Discussionmentioning

confidence: 99%

Case Report: Human Dermatitis Linked to Ornithonyssus bursa (Dermanyssoidea: Macronyssidae) Infestation in Portugal

2020

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Ornithonyssus bursa (Berlese, 1888), also known as the tropical fowl mite, is a blood-feeding ectoparasite of domestic and wild birds. It is considered a serious pest to poultry in warm and tropical climates and has been reported to attack humans, causing gamasoidosis. Avian-mite dermatitis may be difficult to recognize and misdiagnosed as bites from other arthropods. The present report describes two cases of human dermatitis linked to O. bursa infestation. Both cases occurred in an apartment in a residential area in Oeiras, Portugal, where two members of the same family presented with pruritic erythematous skin eruptions disseminated over their body over a period of 4 months. The lesions were located mostly in the pelvic, gluteal, coccygeal, and perineal regions, and also on the neck, wrists and torso. On inspecting the mattresses and the covers of the bedrooms, three mites ~1 mm long by 0.5 mm wide were found. The three specimens were identified as O. bursa. Investigations tracing back the source of infestation, revealed that the mites were carried over from chickens raised 45 km away (Sesimbra, Setúbal) that, after being slaughtered for consumption, had been transported by car to the apartment in Oeiras. The chickens were farmed in an open backyard henhouse that allowed free access to several species of wild birds, including pigeons (Columba livia), turtle doves (Streptopelia decaocto), and sparrows (Passer domesticus). Recent reports suggest that O. bursa may be established in Mediterranean countries, increasing the risk of gamasoidosis. This is the first record linking O. bursa infestation of chickens with human dermatitis in continental Portugal. More research is needed to assess the extent of poultry infestation and evaluate the possible implications for the poultry industry, as well as for human health in Portugal.

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Clinical gamasoidosis and antibody response in two patients infested with Ornithonyssus bursa (Acari: Gamasida: Macronyssidae)

Cited by 15 publications

References 42 publications

Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin

Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin

Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Case Report: Human Dermatitis Linked to Ornithonyssus bursa (Dermanyssoidea: Macronyssidae) Infestation in Portugal

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