Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan

Chen, Yueh-Ling; Kang, Eugene Yu-Chuan; Yeh, Lung‐Kun; David, H. K.; Tan, Hsin-Yuan; Chen, Hung-Chi; Hung, Kuo-Hsuan; Huang, Yhu-Chering; Hsiao, Ching-Hsi

doi:10.3390/antibiotics10121445

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Of the 17 (88.2%) MRSA isolates, 15 that carried type IV or V SCC mec belonged to CA-MRSA based on molecular definition. We did not report the genotyping of other MSSA isolates here because they had quite diverse molecular characteristics [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features

Hsiao

Kang

Yeh

et al. 2022

IJMS

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Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton–Valentine leucocidin (PVL), a major virulence factor of S. aureus. We recruited 49 patients with culture-proven S. aureus keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCCmec VT/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(−) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for S. aureus keratitis.

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Section: Resultsmentioning

confidence: 99%

Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features

Hsiao

Kang

Yeh

et al. 2022

IJMS

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“…Despite these limitations, this study contributes to our current knowledge about staphylococci in Taiwan, complementing our previous studies about the clinical and microbiological characteristics of S. aureus ocular infections. 44 , 49 Through our clinical, genotypic, and phenotypic characterization of ocular S. epidermidis isolates, we were able to identify certain pathogenic clonal lineages and their relevant virulence factors.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Ocular Methicillin-Resistant Staphylococcus epidermidis Isolates in Taiwan

Chang,

Huang,

Chen

et al. 2023

Invest. Ophthalmol. Vis. Sci.

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Purpose Staphylococcus epidermidis , a commensal, has emerged as an important opportunistic pathogen, particularly methicillin-resistant S. epidermidis (MRSE). The mechanism behind this transformation remains unclear. This study aimed to investigate the molecular and phenotypic characteristics of MRSE isolated from healthy conjunctiva and ocular infections. Methods We collected MRSE isolates from two groups: healthy conjunctiva from patients undergoing cataract surgeries and ocular infections at our hospital. Genotypic analysis included pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCC mec ), and biofilm-related genes ( ica A, aap , and bhp ). Additionally, phenotypic data on biofilm production and antibiotic susceptibility were recorded. Results A total of 86 isolates, including 42 from healthy conjunctiva and 44 from ocular infections, were analyzed. MLST identified 21 sequence types (STs), with ST59 being the most frequent ( n = 33, 39.5%), followed by ST130 ( n = 10, 11.6%), ST57 ( n = 6, 7.0%), and ST2 ( n = 6, 7.0%). All isolates were categorized in 23 PFGE types, and SCC mec IV was the most prevalent SCC mec type ( n = 52, 60.5%). The two sources of isolates exhibited overlapping molecular types and phenotypic traits, although the ocular infection isolates exhibited significantly higher multidrug resistance compared to healthy conjunctiva isolates ( P = 0.032). When contrasting ST59 with non-ST59, ST59 displayed a significantly higher presence of aap (100%) and bhp (69.7%) while lacking ica A (0%). ST59 also showed lower susceptibility to fluoroquinolones compared to non-ST59 (42.4%–54.5% vs. 75.5%–83.0%; P < 0.01). Conclusions MRSE isolates from healthy conjunctiva and ocular infections demonstrated a degree of resemblance. Specific strains, notably ST59, exhibited distinctive characterizations.

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“…This shows the risk associated with failure of antimicrobial therapy and, consequently, fatal conditions due to excessive pathogen proliferation, which can lead to visual organ loss. In contrast, knowing that Gram-positive bacteria are reported as the main causative agents of ocular infections, the detection of methicillin-resistant S. aureus (MRSA) strains is of utmost relevance, as they are epidemiologically relevant due to their high resistance to methicillin and other β-lactam antibiotics [21,22].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Bacterial Agents Causing External Ocular Infections Isolates of Patients in a Third Level Hospital

Durán-Manuel,

Bello-López,

Salinas-Bobadilla

et al. 2023

Pathogens

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Empirical use of antibiotics in the treatment of eye infections leads to bacterial pathogens becoming resistant to antibiotics; consequently, treatment failure and eye health complications occur. The aim of this study was to describe the phenotype and genotype of the resistance and adherence of bacterial agents causing eye infections in patients at Hospital Juárez de México. An observational, prospective, cross-sectional, and descriptive study was carried out in patients with signs and symptoms of ocular infection. Bacterial agents were isolated and identified by classical microbiology and mass spectrometry. Antibiotic resistance and adherence profiles were determined. Finally, resistance (mecA/SCCmec) and virulence (icaA and icaD) genes were detected in the Gram-positive population. The results showed that blepharitis was the most prevalent condition in the study population. A MALDI-TOF analysis revealed that Staphylococcus and Pseudomonas genus were the most prevalent as causal agents of infection. Resistances to β-lactams were detected of 44 to 100%, followed by clindamycins, aminoglycosides, folate inhibitors, and nitrofurans. A multiple correspondence analysis showed a relationship between mecA genotype and β-lactams resistance. The identification of SCCmecIII and SCCmecIV elements suggested community and hospital sources of infection. Finally, the coexistence of icaA+/icaD+/mecA(SCCmecIII) and icaA+/icaD+/mecA(SCCmecIV) genotypes was detected in S. aureus. The identification of resistant and virulent isolates highlights the importance of developing protocols that address the timely diagnosis of ocular infections. Herein, implications for the failure of antimicrobial therapy in the treatment of ocular infections in susceptible patients are analysed and discussed.

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Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan

Cited by 5 publications

References 31 publications

Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features

Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features

Molecular and Phenotypic Characterization of Ocular Methicillin-Resistant Staphylococcus epidermidis Isolates in Taiwan

Molecular Characterization of Bacterial Agents Causing External Ocular Infections Isolates of Patients in a Third Level Hospital

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