Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm

Fugger, Edward F.

doi:10.1016/s0093-691x(99)00228-9

Cited by 49 publications

(34 citation statements)

References 11 publications

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“…Offspring of predetermined sex using FC have been successfully produced using fresh and frozen-thawed spermatozoa in several mammalian species: pigs, 162 cattle, 163 sheep 164 bottlenose dolphins, 165 goat 166 and humans. 167 Further refinements to standard FC technology included replacement of the beveled needle by an orientating nozzle with a ceramic tip giving sperm less time to lose orientation, which improved correct orientation of sperm to 70%. 168 Conversion to highspeed modified FC operating under increased pressure had improved the accuracy and efficiency (20 000-25 000 events per second) of sperm sorting and purity of sorted population.…”

Section: Detection Of Oxidative Stress and Lipid Peroxidationmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

139

120

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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Section: Detection Of Oxidative Stress and Lipid Peroxidationmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

139

120

View full text Add to dashboard Cite

show abstract

“…The production of offspring using sex-sorted spermatozoa in conjunction with in vitro fertilisation (IVF) and embryo transfer (ET) has been reported in cattle (Cran et al 1993, 1995, Fry et al 2004, pigs (Rath et al 1997, 1999, Abeydeera et al 1998, Johnson et al 2000, humans (Levinson et al 1995, Fugger et al 1998, Fugger 1999) and sheep (intra-cytoplasmic sperm injection; Catt et al 1996). Improvements in sorting efficiency (Rens et al 1998, 1999) and cryopreservation methods (Schenk et al 1999) for sex-sorted spermatozoa facilitated the production of offspring after low-dose artificial insemination (AI) in cattle , sheep (Hollinshead et al 2002a), horses (Lindsey et al 2002) and elk (Schenk & DeGrofft 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Improvements in sorting efficiency (Rens et al 1998, 1999) and cryopreservation methods (Schenk et al 1999) for sex-sorted spermatozoa facilitated the production of offspring after low-dose artificial insemination (AI) in cattle , sheep (Hollinshead et al 2002a), horses (Lindsey et al 2002) and elk (Schenk & DeGrofft 2003).…”

Section: Introductionmentioning

confidence: 99%

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

Hollinshead

Evans

et al. 2004

Reproduction

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The characteristics and functional capacity of ram spermatozoa frozen-thawed prior to and after flow cytometric sorting was assessed after incubation (37 8C; 6 h), in vitro fertilisation (IVF), and transfer of fresh and vitrified in vitro produced embryos. Frozen-thawed spermatozoa from two rams were allocated to four treatment groups: (i) non-sorted (Control); (ii) sorted (FS); (iii) sorted then re-frozen (FSF) and (iv) re-frozen control (FCF). Frozen-thawed samples were separated into X-and Y-chromosome bearing spermatozoa using a high-speed sperm sorter after density gradient centrifugation (X: 88 6 1.5% and Y: 87 6 1.1% purity). After 6 h incubation (37 8C), the percentage of motile spermatozoa was higher (P < 0.001) for FS (84 6 2.0%) compared with all other treatments (Control: 36 6 3.3%, FSF: 28 6 3.1%, FCF: 20 6 2.0%). In a sperm migration test greater numbers of FS spermatozoa penetrated 5 mm into the artificial cervical mucus compared with spermatozoa from all other treatments (152 6 39.4 vs 31 6 9.2 spermatozoa respectively; P < 0.05). Fertilisation and cleavage rates were higher (P < 0.05) for in vitro matured oocytes inseminated with Control compared with FSF spermatozoa. However, the Day 7 blastocyst development rate was higher for oocytes inseminated with FSF (62.2%) than FS and Control spermatozoa (52.7 and 50.0%; P < 0.05). The number of ewes pregnant (Day 60), lambing and the in vivo embryo survival rate was greater (P < 0.01) after the transfer of fresh embryos rather than vitrified embryos derived from X-and Y-spermatozoa (67.6, 64.7 and 41.2% vs 29.6, 25.9 and 14.8% respectively).Twenty-six of the 30 (86.7%) lambs derived from sex-sorted spermatozoa were of the correct sex. These results demonstrate that frozen-thawed ram spermatozoa can be sex-sorted for immediate or future use after re-cryopreservation and, in conjunction with IVF and embryo transfer, can be used to efficiently produce offspring of pre-determined sex.

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“…Meanwhile, other researchers believed that this method was ineffective (17,23). However, Fugger's study suggests that the health of the baby after delivery has been confirmed by this method (24). In subjects aged between 20 to 40 years, the results showed that there was no significant difference between successful and unsuccessful groups, which was not referred to in other studies.…”

Section: Discussionmentioning

confidence: 87%

Investigating the Success Rate of Sex Selection in Cycles of Intrauterine Insemination of Sperm Using an Albumin Gradient Method in Infertile Couples Referring to the Omid Persian Gulf Infertility Center of Bushehr, Iran

Ahmadi¹,

Rahmani²,

Motamed³

et al. 2016

International Journal of Women’s Health and Reproduction Scienc

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Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm

Cited by 49 publications

References 11 publications

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

Investigating the Success Rate of Sex Selection in Cycles of Intrauterine Insemination of Sperm Using an Albumin Gradient Method in Infertile Couples Referring to the Omid Persian Gulf Infertility Center of Bushehr, Iran

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