Clinical Evaluation of the FilmArray Blood Culture Identification Panel in Identification of Bacteria and Yeasts from Positive Blood Culture Bottles

Altun, Osman; Almuhayawi, Mohammed S.; Ullberg, Måns; Özenci, Volkan

doi:10.1128/jcm.01835-13

Cited by 247 publications

(222 citation statements)

References 23 publications

(21 reference statements)

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…86 In a study reported by Altun et al, the test covered all microorganisms in 91.6% of positive BC bottles, and it had the potential to identify multiple pathogens simultaneously from positive BC with polymicrobial growth. 87 This approach performs the extraction, amplification, and detection in a closed diagnostic system, minimizing contamination. It is a low-complexity system for the operator, requiring only injection of the BC sample into the pouch and starting the instrument; hence, the laboratory procedures can be performed by personnel with no training in molecular techniques.…”

Section: Filmarray ®mentioning

confidence: 99%

See 1 more Smart Citation

In vitro diagnosis of sepsis: a review

Marcello

Tumolo²,

Donno³

et al. 2016

PLMI

View full text Add to dashboard Cite

Sepsis, severe sepsis and septic shock, systemic inflammatory response, and other related manifestations represent a relevant medical problem with high morbidity and mortality, despite the improvements in diagnosis, treatment, and preventive measures over the last few decades. The limited knowledge of the pathophysiology in association with the lack of in vitro diagnostic methods for the certain and quick determination of the causative microbiological agents and their antibiotic resistance means the condition is still critical and of high impact in health care. The current gold standard method to detect the sepsis-causing pathogens, which is based on blood culture, is still insufficiently sensitive and slow. The new culture-independent molecular biology-based techniques can lead to the identification of a broad range of microorganisms and resistance markers within a few hours and with high sensitivity and specificity; nevertheless, limitations of, for example, the polymerase chain reaction-based methods still hamper their application in the clinical routine. This review summarizes the in vitro diagnostic methods and their approach in the clinical diagnosis of the bloodstream infections, and explores their advantages and disadvantages at the current state of the art. A quick analysis of the future prospective in multiplex technologies for microbiological diagnosis of sepsis is also provided.

show abstract

Section: Filmarray ®mentioning

confidence: 99%

“…86,87 The assay is based on the extraction and purification of nucleic acids from positive BC and amplification of the target genes by a reverse transcriptase first-stage PCR. 88 This simple system requires just a short hands-on time, with a total run time of ∼1 hour, and only one sample can be analyzed at a time.…”

Section: Filmarray ®mentioning

confidence: 99%

In vitro diagnosis of sepsis: a review

Marcello

Tumolo²,

Donno³

et al. 2016

PLMI

View full text Add to dashboard Cite

show abstract

“…However, the identification of microorganisms from positive bottles with conventional methods may still take up to 48 h for many microorganisms and even longer for others, such as slow-growing bacteria and yeasts (11). Several methods have been evaluated for rapid identification of microorganisms directly from positive blood cultures (12)(13)(14). In contrast, the data on the performance of rapid microbiological methods in diagnostics of SBF are scarce.…”

mentioning

confidence: 99%

Rapid Identification of Microorganisms from Sterile Body Fluids by Use of FilmArray

et al. 2015

Self Cite

View full text Add to dashboard Cite

Early detection and rapid identification of microorganisms are crucial the for appropriate management of infections of normally sterile body fluids (SBF) (1-3). Previously, SBF other than blood were cultured on solid medium (4). Larger volumes were processed through centrifugation and filtration in order to concentrate and improve the detection of microorganisms. Nonetheless, the microorganism recovery from SBF was unsatisfactory until the introduction of blood culture (BC) bottles. The introduction of BC bottles resulted in increased microorganism recovery in addition to decreased time to detection (5-10). However, the identification of microorganisms from positive bottles with conventional methods may still take up to 48 h for many microorganisms and even longer for others, such as slow-growing bacteria and yeasts (11). Several methods have been evaluated for rapid identification of microorganisms directly from positive blood cultures (12)(13)(14). In contrast, the data on the performance of rapid microbiological methods in diagnostics of SBF are scarce. The aim of the present study was to evaluate the performance of the FilmArray blood culture identification (BCID) panel in the identification of microorganisms directly from positive blood culture bottles inoculated with clinical SBF specimens.This study was performed at Karolinska University Hospital, Huddinge, Sweden. SBF specimens were collected in the clinical wards using standard protocols. Specimen volumes of 1 to 2 ml were inoculated in BacT/Alert pediatric/PF plus (bioMérieux, Durham, NC, USA), and volumes of Ͼ2 ml in BacT/Alert aerobic/FA plus and anaerobic/FN plus bottles (bioMérieux). All bottles were incubated in the BacT/Alert 3D blood culture system (bioMérieux) until they gave a positive signal or for a maximum incubation time of 5 days.Positive BC bottles with SBF were processed as follows. Gram staining was performed immediately after bottles signaled positivity. All positive bottles were subcultured onto a Columbia blood agar plate (aerobic and anaerobic), a cystinelactose-electrolyte-deficient plate (CLED) agar, and a chocolate agar plate. After an incubation time of 48 h, we analyzed the growth morphology on the agar plates. Identification of microorganisms was done by conventional methods, including matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics, Bremen, Germany) and Vitek2 XL (bioMérieux). Susceptibility testing was performed by the disc diffusion method according to EUCAST guidelines (http://www.eucast.org/). Methicillin resistance in Staphylococcus spp. was evaluated by the cefoxitin disc diffusion method. Similarly, imipenem and meropenem disc diffusion methods were used in order to determine/confirm the absence of Klebsiella pneumoniae carbapenemase (KPC). FilmArray (FA; bioFire, Salt Lake City, UT) analyses were performed as previously described (13). Results that were discrepant between the reference method and the FilmArray were evaluated further. According to the discre...

show abstract

“…Th ese include matrix-assisted laser desorption/ionization time of fl ight (1), DNA hybridization (2), and polymerase chain reaction (3,4). Th e Verigene Gram-Positive Blood Culture (BC-GP) assay (Nanosphere, Inc., Northbrook, IL) is a multiplex, DNA hybridization assay that detects 3 genera, 9 species, and 3 resistance markers directly from blood cultures positive for gram-positive organisms.…”

mentioning

confidence: 99%