Clinical evaluation of the BioFire Global Fever Panel for the identification of malaria, leptospirosis, chikungunya, and dengue from whole blood: a prospective, multicentre, cross-sectional diagnostic accuracy study

Manabe, Yukari C.; Betz, Joshua; Jackson, Olivia; Asoala, Victor; Bazán, Isabel; Blair, Paul W; Chang, Aileen Y.; Chusri, Sarunyou; Crump, John A.; Edgel, Kimberly A.; Faix, Dennis J.; Fernandez, Stefan; Fox, Anne T.; Garcia, Jose; Grögl, Max; Hansen, Erin; Heang, Vireak; House, Stacey L.; Jongsakul, Krisada; Kaburise, Michael B; Klungthong, Chonticha; Lamorde, Mohammed; Letizia, Andrew G.; Lorenzana, Ivette; Luy, Malen; Maro, Vanance P; Mores, Christopher N.; Myers, Christopher A.; Oduro, Abraham; Parham, Leda; Porzucek, Abigail J; Prouty, Michael; Rabiger, David S; Rubach, Matthew P.; Siles, Crystyan; Silva, Maria João; Ukachu, Chinaka; Waitumbi, John N.; Phillips, Cynthia L.; Jones, Brian W.

doi:10.1016/s1473-3099(22)00290-0

Cited by 17 publications

(8 citation statements)

References 26 publications

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“…Our study identified a case of CCHF that otherwise would not have been detected. Similarly, CCHF was previously identified at the Global Fever panel febrile illness study site in Mubende, Uganda 12,23 . During the 2022 Sudan virus disease outbreak.…”

Section: Discussionmentioning

confidence: 57%

“…If HIV testing was positive, urine lipoarabinomannan (Abbott TB LAM Ag, Illinois, United States) testing was performed. The Biofire FilmArray Global Fever polymerase chain reaction (PCR) panel (Biofire, Utah, USA) was run on whole blood samples to identify 19 bacterial, viral, or protozoal targets or plasma if whole blood was unavailable (first 36 participants) 12 . Plasma samples were run in duplicate with a Crimean-Congo hemorrhagic fever virus real-time RT-PCR assay targeting the NP gene using previously described methods [13][14][15] but replaced after transition of the Global Fever panel from off-site to on-site use after the first 237 participants.…”

Section: Methodsmentioning

confidence: 99%

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Long-term Mortality Among Hospitalized Adults with Sepsis in Uganda: a Prospective Cohort Study

Blair,

Okello,

Wailagala

et al. 2023

Preprint

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BackgroundTwelve-month mortality in sepsis survivors has not been previously characterized in sub-Saharan Africa.MethodsHospitalized adults with ≥ 2 modified systemic inflammatory response syndrome (SIRS) criteria (temperature < 36°C or > 38°C, heart rate ≥ 90 beats per minute, or respiratory rate ≥ 20 breaths per minute) were enrolled at a tertiary care centre from October 2017 to August 2022. Multiple clinical blood and respiratory molecular and antigen assays were used to identify infectious etiologies. Baseline demographics were evaluated for risk of death by 1 month and 12 months using Cox proportional hazards regression.ResultsAmong 435 participants, the median age was 45.0 years (interquartile range [IQR]: 28.0, 60.0) years, 57.6% were female, and 31.7% were living with HIV. Malaria (17.7%) followed by tuberculosis (4.7%), and bacteremia (4.6%) were the most common detected causes of illness. Overall, 49 (11.3%) participants died, and 24 participants died between one month and one year (49.0% of deaths and 5.5% of the cohort). Female participants had a decreased risk of death by 12-months (unadjusted hazard ratio [HR]: 0.37; 95% confidence interval [CI]: 0.21 to 0.66).ConclusionsThe burden of sepsis may be underestimated in sub-Saharan Africa due to limited long-term follow-up.

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Section: Discussionmentioning

confidence: 57%

Section: Methodsmentioning

confidence: 99%

Long-term Mortality Among Hospitalized Adults with Sepsis in Uganda: a Prospective Cohort Study

Blair,

Okello,

Wailagala

et al. 2023

Preprint

Self Cite

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“…The global population growth during this period might have outpaced the increase in malaria cases, contributing to the decline in ASIR. Besides the gold standard for diagnosing malaria – microscopy of thick and thin blood films [ 24 ], other methods include malaria rapid diagnostic tests [ 25 ], parasite lactate dehydrogenase (pLDH) rapid diagnostic tests [ 24 ], and nucleic acid testing [ 26 ]. As diagnostic criteria and technologies have evolved over time, they have impacted the reporting of malaria cases, which in turn might have led to more accurate and widespread detection of malaria cases, consequently influencing the total reported case numbers.…”

Section: Discussionmentioning

confidence: 99%

Association of temperature and precipitation with malaria incidence in 57 countries and territories from 2000 to 2019: A worldwide observational study

Liu,

Wang,

Deng

et al. 2024

J Glob Health

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Background The transmission of malaria is known to be affected by climatic factors. However, existing studies on the impact of temperature and precipitation on malaria incidence offer no clear-cut conclusions, and there is a lack of research on a global scale. We aimed to estimate the association of temperature and precipitation with malaria incidence globally from 2000 to 2019. Methods We used meteorological data from the National Centers for Environmental Information and malaria incidence data from the Global Burden of Disease Study 2019 to calculate effect sizes through quasi-Poisson generalised linear models while controlling for confounders. Results 231.4 million malaria cases occurred worldwide in 2019. National annual average temperature and precipitation were associated with malaria incidence, with an increase in the age-standardised incidence rate (ASIR) of 2.01% (95% confidence interval (CI) = 2.00, 2.02) and 6.04% (95% CI = 6.00, 6.09) following one unit increase of national annual average temperature and precipitation. In subgroup analysis, we found that malaria incidence in Asian countries was most affected by temperature, while the incidence in African countries was most affected by precipitation ( P < 0.05). Stratified by age, children under five were most affected by both temperature and precipitation ( P < 0.05). We additionally found that the impact of the national annual average temperature on malaria incidence increased over time ( P < 0.05). Conclusions We advocate for a comprehensive approach to malaria prevention, focussed on addressing the impact of climate factors through international collaboration, adaptive measures, and targeted interventions for vulnerable populations.

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“…There are several commercial nucleic acid amplification assays designed for use in acute febrile illness etiology determination that aim to identify a broad set of etiologies. These include the BioFire FilmArray Global Fever Panel (19 pathogens)[ 45 ], Verigene, VerePLEX (26 pathogens)[ 46 ], and FeverDisk (12 pathogens) [ 47 ]. We selected custom-made cards as the ability to select pathogens of regional interest was seen as an important asset in responding to local clinicians’ needs assessment, and because acute febrile illness surveillance and emerging infectious diseases surveillance are overlapping tasks in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

Etiology of acute febrile illness in the peruvian amazon as determined by modular formatted quantitative PCR: a protocol for RIVERA, a health facility-based case-control study

et al. 2023

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Background The study of the etiology of acute febrile illness (AFI) has historically been designed as a prevalence of pathogens detected from a case series. This strategy has an inherent unrealistic assumption that all pathogen detection allows for causal attribution, despite known asymptomatic carriage of the principal causes of acute febrile illness in most low- and middle-income countries (LMICs). We designed a semi-quantitative PCR in a modular format to detect bloodborne agents of acute febrile illness that encompassed common etiologies of AFI in the region, etiologies of recent epidemics, etiologies that require an immediate public health response and additional pathogens of unknown endemicity. We then designed a study that would delineate background levels of transmission in the community in the absence of symptoms to provide corrected estimates of attribution for the principal determinants of AFI. Methods A case-control study of acute febrile illness in patients ten years or older seeking health care in Iquitos, Loreto, Peru, was planned. Upon enrollment, we will obtain blood, saliva, and mid-turbinate nasal swabs at enrollment with a follow-up visit on day 21–28 following enrollment to attain vital status and convalescent saliva and blood samples, as well as a questionnaire including clinical, socio-demographic, occupational, travel, and animal contact information for each participant. Whole blood samples are to be simultaneously tested for 32 pathogens using TaqMan array cards. Mid-turbinate samples will be tested for SARS-CoV-2, Influenza A and Influenza B. Conditional logistic regression models will be fitted treating case/control status as the outcome and with pathogen-specific sample positivity as predictors to attain estimates of attributable pathogen fractions for AFI. Discussion The modular PCR platforms will allow for reporting of all primary results of respiratory samples within 72 h and blood samples within one week, allowing for results to influence local medical practice and enable timely public health responses. The inclusion of controls will allow for a more accurate estimate of the importance of specific prevalent pathogens as a cause of acute illness. Study Registration Project 1791, Registro de Proyectos de Investigación en Salud Pública (PRISA), Instituto Nacional de Salud, Perú.

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Clinical evaluation of the BioFire Global Fever Panel for the identification of malaria, leptospirosis, chikungunya, and dengue from whole blood: a prospective, multicentre, cross-sectional diagnostic accuracy study

Cited by 17 publications

References 26 publications

Long-term Mortality Among Hospitalized Adults with Sepsis in Uganda: a Prospective Cohort Study

Long-term Mortality Among Hospitalized Adults with Sepsis in Uganda: a Prospective Cohort Study

Association of temperature and precipitation with malaria incidence in 57 countries and territories from 2000 to 2019: A worldwide observational study

Etiology of acute febrile illness in the peruvian amazon as determined by modular formatted quantitative PCR: a protocol for RIVERA, a health facility-based case-control study

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