Clinical Evaluation of Specific Immunoglobulin E in Sputum in Pediatric Patients

Young, A; Seok, ChoiBong

doi:10.1089/ped.2017.0864

Cited by 3 publications

(5 citation statements)

References 17 publications

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“…In the present study, we found a highly significant correlation (rho, 0.498; p = 0.000) between total IgE levels in serum and sputum in our overall sample, a finding that is consistent with the results described by Manise et al in asthmatic patients [28]. By contrast, other authors, including Mouthuy et al [7] and Ahn et al [33] have not found any correlation between total IgE levels in sputum and serum. These contrasting findings raise further doubts about the sensitivity of the ImmunoCap technique used to measure IgE in sputum, potentially providing an additional explanation for the differences between our results and those of Mouthuy et al…”

Section: Correlation Between Ige In Sputum and Serumsupporting

confidence: 92%

Total and specific immunoglobulin E in induced sputum in allergic and non-allergic asthma

et al. 2020

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Background Most patients with nonallergic asthma have normal serum immunoglobulin E (IgE) levels. Recent reports suggest that total and aeroallergen-specific IgE levels in induced sputum may be higher in nonallergic asthmatics than in healthy controls. Our objective is to compare total and dust-mite specific (Der p 1) IgE levels in induced sputum in allergic and nonallergic asthmatics and healthy controls. Methods Total and Der p 1-specific IgE were measured in induced sputum (ImmunoCAP immunoassay) from 56 age-and sex-matched asthmatics (21 allergic, 35 nonallergic) and 9 healthy controls. Allergic asthma was defined as asthma with a positive prick test and/or clinicallysignificant Der p 1-specific serum IgE levels. Results Patients with allergic asthma presented significantly higher total and Der p 1-specific serum IgE levels. There were no significant between-group differences in total sputum IgE. However, Der p 1-specific sputum IgE levels were significantly higher (p = 0.000) in the allergic asthmatics, but without differences between the controls and nonallergic asthmatics. Serum and sputum IgE levels were significantly correlated, both for total IgE (rho = 0.498; p = 0.000) and Der p 1-specific IgE (rho, 0.621; p = 0001). Conclusions Total IgE levels measured in serum and induced sputum are significantly correlated. No significant differences were found between the differents groups in total sputum IgE. Nevertheless, the levels of Der p 1-specific sputum IgE levels were significantly higher in the allergic asthmatics, but without differences between the controls and nonallergic asthmatics. Probably due to the lack of sensitivity of the test used, but with the growing evidence for local

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Section: Correlation Between Ige In Sputum and Serumsupporting

confidence: 92%

Total and specific immunoglobulin E in induced sputum in allergic and non-allergic asthma

et al. 2020

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“…The prescription of omalizumab is based on serum IgE levels which our data suggest may not be reflective of the airway inflammatory profile. Studies which have compared induced sputum levels of IgE to that of serum IgE have shown contradictory reports with some showing a correlation between the two levels, while others have shown no correlation between the two compartments [ 19 – 22 ]. Furthermore, studies which use more invasive measures of airway sampling would suggest that IgE levels are higher in the airways which may indicate localized production of IgE not reflected in the serum [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, both BAL and blood samples were acquired on the same day and analyzed in a similar manner to minimize potential confounders. We acknowledge that bronchoscopy may not be viable in all centers or in all patients who are being screened prior to starting monoclonal therapy; however, studies on the relationship between sputum and blood IgE have been contradictory [ 19 – 22 ]. Bronchoscopy offers an advantage in that it allows for visual inspection and guarantees sample acquisition to fully phenotype patients prior to starting monoclonals.…”

Section: Discussionmentioning

confidence: 99%

Airway and Systemic Immunoglobulin Profiling and Immune Response in Adult Asthma

Walsh,

Sullivan,

Ward

et al. 2024

Lung

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Introduction Immunoglobulins play a vital role in host immune response and in the pathogenesis of conditions like asthma. Therapeutic agents such as monoclonal antibodies target specific elements of the asthmatic inflammatory cascade. Decisions to utilize these medications are often based on systemic inflammatory profiling without direct insight into the airway inflammatory profile. We sought to investigate the relationship between immunoglobulin and cytokine profiles in the airway and systemic immune compartments of adult asthmatics. Methods Blood sampling and bronchoscopy with bronchoalveolar lavage (BAL) were performed in 76 well-defined adult asthmatics. Antibody and cytokine profiles were measured in both BAL and serum using ELISA and quantibody arrays. Results There was no relationship between BAL and serum levels of IgE. This is of significance in an asthma population. For some analytes, correlation analysis was significant (P < 0.05) indicating representativeness of our cohort and experimental setup in those cases. Nevertheless, the predictive power (r2) of the BAL-to-serum comparisons was mostly low except for TNF-α (r2 = 0.73) when assuming a simple (linear) relationship. Conclusion This study highlights the importance of sample site when investigating the roles of immunoglobulins and cytokines in disease pathogenesis and suggests that both localized and systemic immune responses are at play. The prescription of asthma monoclonal therapy is generally based on systemic evaluation of cytokine and immunoglobulin levels. Our research suggests that this approach may not fully reflect the pathophysiology of the disease and may provide insight into why some patients respond to these targeted therapies while others do not.

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“…We gauged responses at the fifteenth minute. We defined the positive reaction as ≥3 mm above the negative control [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…We obtained peripheral blood eosinophil counts from hemogram parameters and measured total IgE from serum (by nephelometric method with a machine from Siemens Healthcare Diagnostics Products, Marburg, Germany) and assayed causal or suspected food (milk, egg white, egg yolk, peanut, hazelnut, walnut, wheat, fish, soy) and aero ( Dermatophagoides farinea , Dermatophagoides pteronyssinus , Alternaria, alternata , Aspergillus , cockroaches mix, cat and dog dander, and grass-mix and tree-mix pollens) allergen-specific IgE levels with the Immuno-CAP system (UniCAP; Uppsala, Sweden) (≥0.35 kIU/L was considered to be positive). The patient’s atopy condition was described by positivity to at least one allergen by skin prick test (SPT; wheal ≥3 mm larger than negative control) or specific IgE testing (≥0.35 kIU/L) [ 20 ]. Individuals with no positive results were classified as non-atopic (non-sensitized).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Impact of Serum Vitamin D Levels on the Scoring Atopic Dermatitis Index in Pediatric Atopic Dermatitis

Çiçek,

Köle

2023

Children

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Atopic dermatitis (AD) is a chronic and recurrent inflammatory skin condition characterized by itching, eczematous plaques, and dry skin. Despite ongoing research, its exact cause remains elusive. In this study, we aimed to explore the factors that influence the severity of AD in children and assess the relationship between serum vitamin D levels and the disease’s severity. We enrolled 96 AD patients in our investigation, evaluated their clinical condition using the Scoring Atopic Dermatitis (SCORAD) index, and compared them to a group of 90 healthy controls. Our analysis revealed that serum vitamin D levels and eosinophil counts significantly impacted the SCORAD index (p < 0.001). According to standardized regression coefficients, for each incremental unit in serum vitamin D levels, the SCORAD index exhibited a decrease of 0.449 units. Similarly, a one-unit increase in eosinophil count resulted in a 0.009 unit increase in the SCORAD index. It is worth noting that the influence of serum vitamin D levels on disease severity surpasses that of eosinophil counts and atopic conditions. In our patient cohort, we uncovered a negative correlation (r = −0.419, p < 0.001) between serum vitamin D levels and the SCORAD index. Our findings suggest that low serum vitamin D levels may have a more substantial impact on AD severity than atopic conditions and eosinophilia. Furthermore, we observed a negative association between the severity of AD and serum 25(OH)D3 levels.

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Clinical Evaluation of Specific Immunoglobulin E in Sputum in Pediatric Patients

Cited by 3 publications

References 17 publications

Total and specific immunoglobulin E in induced sputum in allergic and non-allergic asthma

Total and specific immunoglobulin E in induced sputum in allergic and non-allergic asthma

Airway and Systemic Immunoglobulin Profiling and Immune Response in Adult Asthma

Evaluation of the Impact of Serum Vitamin D Levels on the Scoring Atopic Dermatitis Index in Pediatric Atopic Dermatitis

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