Clinical Evaluation of Kinetic Enzymatic Fixed-Time and Integral Analysis of Serum Triglycerides

Tiffany, T. O.; Morton, J. M.; Hall, Elizabeth M.; Garrett, AS

doi:10.1093/clinchem/20.4.476

Cited by 42 publications

(4 citation statements)

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“…TG are converted to free glycerol by lipase, and the glycerol is oxidized (by glycerol dehydrogenase) to dihydroxy-acetone under formation of NADH, which is measured using a kinetic, bichromatic method. Preexisting glycerol (usually below 0.1 mmol/L) is included in the result (28).…”

Section: Methodsmentioning

confidence: 99%

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹

Boot¹,

Engels²,

Boerma

et al. 1997

The Journal of Clinical Endocrinology & Metabolism

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Adults with childhood onset GH deficiency (GHD) have reduced bone mass, increased fat mass, and disorders of lipid metabolism. The aim of the present study was to evaluate bone mineral density (BMD), bone metabolism, body composition, and lipid metabolism in GHD children before and during 2-3 yr of GH treatment (GHRx). Forty children with GHD, mean age 7.9 yr, participated in the study of bone metabolism and body composition; and an additional group of 17 GHD children, in the study of lipid metabolism. Lumbar spine BMD, total body BMD, and body composition were measured with dual-energy x-ray absorptiometry. Volumetric BMD (bone mineral apparent density, BMAD) was calculated to correct for bone size. BMD, BMAD, lean tissue mass, bone mineral content, fat mass, and percentage body fat were expressed as SD scores (SDS), in comparison with normative data of the same population. Lumbar spine BMD and BMAD and total body BMD were all decreased at baseline. All BMD variables increased significantly during GHRx, lumbar spine BMD SDS, already after 6 months of treatment. Lean tissue mass SDS increased continuously. Bone mineral content SDS started to increase after 6 months GHRx. Fat mass SDS decreased during the first 6 months of GHRx and remained stable thereafter. Biochemical parameters of bone formation and bone resorption did not differ from normal at baseline and increased during the first 6 months of GHRx. Serum 1,25 dihydroxyvitamin D increased continuously during GHRx, whereas PTH and serum calcium remained stable. Lipid profile was normal at baseline: Atherogenic index had decreased and apolipoprotein A1(Apo-A1) had increased after 3 yr of treatment. In conclusion, children with GHD have decreased bone mass. BMD, together with height and lean tissue mass, increased during GHRx. GHRx had a beneficial effect on lipid metabolism.

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Section: Methodsmentioning

confidence: 99%

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹

Boot¹,

Engels²,

Boerma

et al. 1997

The Journal of Clinical Endocrinology & Metabolism

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“…The diabetic rabbits exhibiting persistent blood sugar level in the range of 250-300 mg/dl were selected to determine efficacy of the formulation. Alloxan was used since it produce hyperglycemia ,hypercholesterolemia and elevated lipid peroxidases levels [19] which is the appropriate model for the present study.…”

Section: Induction Of Diabetes-mentioning

confidence: 99%

Assessment of antihyperglycemic and antihyperlipidemic activity of common ayurvedic formulation in alloxan- induced diabetic rabbits.

Z.A¹

2012

IOSR-JDMS

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The present study was planned to evaluate the efficacy of four different ayurvedic formulation for their antihyperglycemic and antihyperlipidemic effect in alloxan induced diabetic rabbits Materials and Methods: Four different ayurvedic formulation (F1,F2,F3,F4)) were administered in different group of alloxan induced diabetic rabbits.Their efficacy, onset and duration of action as antihyperglycemic agents were studied. Their antihyperlipidemic action were also compared. Results: Out of four formulation(F1,F2,F3,F4) studied formulation-F2, showed extremely significant antihyperglycemic activity for satisfactory duration and also showed a significant activity as an antihyperlipidemic agent compared to other three and Tolbutamide. Conclusion: A number of polyherbal formulation are widely marketed claiming to be very effective in diabetes and other associated disease. But, this study indicates that, though there are numerous traditional plants reported to have hypoglycemic properties, many of them proved to be not very effective in lowering blood glucose levels in severe diabetes.

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“…The plasma was separated from the cells and stored in LP tubes at a temperature of -20 o C until analysis. The fasting plasma cholesterol and triglyceride levels were determined by enzymatic method (Roschkau et al, 1975;Sidel et al, 1981) and (Tiffany et al, 1974) respectively using Boehringer-Mannheim kit method.…”

Section: Bleeding Of the Rats And Plasma Cholesterol And Triglyceride...mentioning

confidence: 99%

<i>Afzelia africana</i>, A Novel Non Starch Polysaccharide, Raised Fasting Plasma Cholesterol And Triglyceride Levels Of Rat

Onyechi¹,

Judd²,

Ellis³

2008

Anim. Res. Int.

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The effects o vegetable flour prepared from indigenous plant Afzelia africana, a legume, on the fasting plasma cholesterol and triglyceride levels of rats were investigated. Chemical analysis indicated that Afzelia flour contained significant amount of non-starch polysaccharides (NSP). The flour of Afzelia was incorporated into semi-synthetic diet to provide 10g of dietary fibre which is 300g/kg Afzel a f our. This rep aced some of the casein, oi and starch in the control d et .The test and control diets were ed to young Sprague-Dawley rats for 14 days ad libitum. Food intake, weight gain, crude digestibility, faeca fat excretion, fasting plasma cholesterol and triglyceride were evaluated. The result showed a statist cally significant dif erence (p > 0 05) between the control diet and the test diet in food intake, weight gain and energy digestibility. Afzelia fed rats had a significant higher fasting plasma cho esterol and triglyceride levels than rats fed the control diet. f i l l l i f l i f .

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Clinical Evaluation of Kinetic Enzymatic Fixed-Time and Integral Analysis of Serum Triglycerides

Cited by 42 publications

References 0 publications

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹

Assessment of antihyperglycemic and antihyperlipidemic activity of common ayurvedic formulation in alloxan- induced diabetic rabbits.

<i>Afzelia africana</i>, A Novel Non Starch Polysaccharide, Raised Fasting Plasma Cholesterol And Triglyceride Levels Of Rat

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Clinical Evaluation of Kinetic Enzymatic Fixed-Time and Integral Analysis of Serum Triglycerides

Cited by 42 publications

References 0 publications

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency1

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency1

Assessment of antihyperglycemic and antihyperlipidemic activity of common ayurvedic formulation in alloxan- induced diabetic rabbits.

<i>Afzelia africana</i>, A Novel Non Starch Polysaccharide, Raised Fasting Plasma Cholesterol And Triglyceride Levels Of Rat

Contact Info

Product

Resources

About

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹

Changes in Bone Mineral Density, Body Composition, and Lipid Metabolism during Growth Hormone (GH) Treatment in Children with GH Deficiency¹