Clinical Evaluation of a Novel Urine Collection Kit Using Filter Paper in Neonates: An Observational Study

Abstract

Urine bags are commonly used to collect urine samples from neonates. However, the sample can be contaminated by stool, or detachment of the bag due to body movement can lead to failure of the collection. A qualitative urine collection kit containing ten filter papers of 3.2 mm diameter was developed and clinically verified among 138 neonates. During a single diaper change (approximately 3 h), the rate of urine collection was calculated. Urine collection was considered to be successful if any filter paper in the urine collection sheet turned from blue to white. Of the 127 neonates who passed urine, 122 had a change in the filter paper. The urine collection rate was 96%, with changes in all 10 filter papers observed in 98 neonates (80%). Urine collection rate was not influenced by sex (p = 1.00), age at collection (p = 0.72), preterm birth (p = 1.00), low birth weight (p = 0.92), or fecal contamination (p = 1.00). The incidence of dermatitis was not higher than in the group in which urine bags were used (urine collection kit: 2/68 [3%]; urine bag: 5/68 [7%]; p = 0.44). Novel urine collection kits using filter paper can collect samples from neonates safely and with a high probability of success.

Development of a kit for urine collection on filter paper as an alternative for Pompe disease screening and monitoring by LC-HRMS

“…These results were in accordance with the Glc 4 reference range described by An et al, 2000 (ref. 33) and Piraud et al, 2020, 6 as well as our previous results using liquid urine, 20 evidencing the reliability of the proposed method to quantify the Glc 4 directly in urine samples on lter paper.…”

“…An inclusion list of m / z values for the Glc 4 ( m / z 665.21), acarbose ( m / z 644.24) and creatinine ( m / z 112.05) was used for the PRM experiment. 20…”

“…A previous LC-HRMS analysis of these samples can be found elsewhere. 20 An amount of 1.0 mL of urine was transferred to a piece of lter paper (Whatman 903) cut to 2.5 × 5.0 cm that was placed inside a plastic box (width 2.5 cm × length 5.0 cm). The lter paper was le inside the open box at room temperature (22 °C ± 0.3) for 24 h to ensure complete dryness.…”

“…16 The use of lter paper combined or not with the liquid chromatography-mass spectrometry (LC-MS) technique has been successfully applied for urine screening of different inherited disorders, such as mucopolysaccharidoses, 15 Fabry disease, 17 Gaucher disease, 15 ornithine transcarbamylase deciency, 18 triple H syndrome, 18 Pompe disease, 13 and other clinical evaluations. 20 Although many studies have reported urine specimens dried on lter paper, to our knowledge, no one has proposed a urine collection kit combined with an analysis method for monitoring Glc 4 in Pompe disease.…”

“…Samples were analyzed in the scan range of m/z 100 to 1000 at a resolution of 70 000 FWHM (full width at half maximum) followed by a combination of data-dependent MS/ MS analysis (ddMS2 top3 experiment, normalized collision energy (NCE) 25%), and parallel reaction monitoring (PRM, NCE 12%) analysis, both using the resolution of 17 500 FWHM. An inclusion list of m/z values for the Glc 4 (m/z 665.21), acarbose (m/z 644.24) and creatinine (m/z 112.05) was used for the PRM experiment 20.…”