Clinical evaluation of a newly developed chairside test to determine periodontal pathogens

Abstract

Background The subgingival microbiota as well as determination of markers such as associated pathogens is still in the focus of dental research. The aim of this controlled clinical trial was to determine clinical applicability of a newly developed chairside bacterial test (CST) for the most relevant periodontal pathogens. Methods Within 125 participants (100 with periodontitis, 25 healthy) two sulcus fluid samples each were collected and pooled for further analysis. Samples were analyzed with CST and results (positive signals for every pathogen/control) were visually detected by eye. As a reference quantitative polymerase chain reaction (qPCR) was performed. Results The detection limit of CST revealed 1.2 × 104 for Treponema denticola (T.d.) and Tannerella forsythia (T.f.), 2.5 × 104 for Porphyromonas gingivalis (P.g.), 5.3 × 103 for Prevotella intermedia (P.i.), and 5.8 × 104 for Aggregatibacter actinomycetemcomitans (A.a.). Based on this maximum potential of positive detections, the sensitivities of CST in reference to qPCR were: T.d. (91.3%); T.f. (86.3%); P.g. (83.8%); P.i. (85.7%), and A.a. (100%). In regard to the clinical diagnosis, the CST assay and the qPCR method reached a sensitivity of 87.82% and 94%, respectively. The specificity for both methods was 100%. Conclusion This newly developed CST can detect five typical periodontal pathogens with a somewhat lower sensitivity towards qPCR that can be classified as “good.”

The Impact of Diet, Nutrition and Nutraceuticals on Oral and Periodontal Health

“…These genetic variations can, therefore, favour the progression of the disease, causing the classic trend, characterized by repeated cycles of tissue inflammation, followed by spontaneous remissions (defined as "pousses" trend) [14,15].…”

OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice

“…Furthermore, although the molecular-based detection of oral bacteria has been reported by MyPerioPath ® [ 55 ], HR5 TM (High Risk Pathogen Test from Direct Diagnostics [ 56 ]) and iai PadoTest [ 27 ], all these methods are not chair-side-compatible but laboratory-based. Thus, the samples need to be transported to a laboratory and the results are only available after some days, whereas the OralDisk delivers results in <3 h. The PerioSafe ® and PerioPOC ® [ 57 , 58 ] require only 5 and 20 min, respectively, due to their lateral flow configuration. However, the panels of both tests are limited: for the former, to a single protein biomarker; for the latter, to five periodontitis pathogens.…”

“…c Lateral Flow Test (nucleic acid hybridization). Visual readout provides qualitative analysis [ 58 ].…”

Relationship between Oral Bacterial Count and Postoperative Complications among Patients with Cardiovascular Disease Treated by Surgery: A Retrospective Cohort Study

“…Future studies are needed to identify strains associated with postoperative oral bacterial counts and the incidence of postoperative complications in patients with cardiovascular disease, and to explore cutoff values for oral bacterial abundance in perioperative management and care. Recently, a device to measure the amount of Porphyromonas gingivalis , one of the main components of the red complex, by polymerase chain reaction tests at the chairside was developed [ 27 ], and it is expected to be applied in clinical research.…”