Clinical efficacy of drug-eluting stents in patients with diabetes

Dieter, Robert S.; Ahmed, Mohammed A; Ikram, Sohail; Pacanowski, John P.; Nanjundappa, Aravinda

doi:10.2217/14750708.3.1.1

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…In addition, in vitro and in vivo studies have demonstrated a clear modulation of immune functions by several whey protein-derived products [14]. Furthermore, whey peptides possess immunomodulatory activities, such as stimulating lymphocytes and increasing phagocytosis, and the secretion of immunoglobulin A (IgA) from Peyer's patches [15]. Recently, it has been reported that WP has immunomodulatory properties and the potential to increase host defense [16], protective effects against childhood asthma and atopic syndrome [17], and anticancer effects [18].…”

Section: Introductionmentioning

confidence: 99%

Effects of undenatured whey protein supplementation on CXCL12- and CCL21-mediated B and T cell chemotaxis in diabetic mice

2011

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BackgroundLong and persistent uncontrolled diabetes tends to degenerate the immune system and leads to an increased incidence of infection. Whey proteins (WPs) enhance immunity during early life and have a protective role in some immune disorders. In this study, the effects of camel WP on the chemotaxis of B and T cells to CXCL12 and CCL21 in diabetic mice were investigated.ResultsFlow cytometric analysis of the surface expressions of CXCR4 (CXCL12 receptor) and CCR7 (CCL21 receptor) on B and T cells revealed that the surface expressions of CXCR4 and CCR7 were not significantly altered in diabetic and WP-supplemented diabetic mice compared with control mice. Nevertheless, B and T lymphocytes from diabetic mice were found to be in a stunned state, with a marked and significant (P < 0.05) decrease in CXCL12- and CCL21-mediated actin polymerization and subsequently, a marked decrease in their chemotaxis. WP supplementation in the diabetes model was found to significantly increase CXCL12- and CCL21-mediated actin polymerization and chemotaxis in both B and T cells.ConclusionOur data revealed the benefits of WP supplementation in enhancing cytoskeletal rearrangement and chemotaxis in B and T cells, and subsequently improving the immune response in diabetic mice.

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Section: Introductionmentioning

confidence: 99%

Effects of undenatured whey protein supplementation on CXCL12- and CCL21-mediated B and T cell chemotaxis in diabetic mice

2011

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“…In the case of esophageal squamous cell carcinoma (ESCC) and CDDP, the overexpression of multidrug-resistant protein gene (MRP1/2) ( 9 , 10 ) and the zinc ribbon domain-containing-1 (ZNRD1) ( 7 ) have been reported to be involved in this resistance. A number of methods have been used to identify genes involved in resistance to chemotherapy, including microarray, expression profiling, cDNA library screens, RNAi library screens and small molecule inhibitor screens ( 11 – 13 ).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive screening of genes resistant to an anticancer drug in esophageal squamous cell carcinoma

Tsutsui

Kawakubo

Hayashida

et al. 2015

International Journal of Oncology

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Drug resistance to chemotherapy is a major issue in esophageal cancer management. Drug resistance may be mediated by genetic changes in the tumor; therefore, the identification of gene mutations may lead to better therapeutic outcomes. We used a novel method involving transposons to screen and identify drug-resistant genes. Transposons are DNA sequences that move from one location on the gene to another. A modified piggyBac transposon was designed as an insertion mutagen, and a cytomegalovirus (CMV) promoter sequence was added to induce strong transcription. When the transposon is inserted to the upstream of a certain gene, the gene will be overexpressed while when intserted down or intragenically, it will be downregulated. After establishing a transposon-tagged cell library, we treated cell lines derived from esophageal squamous cell carcinomas (ESCC) [Tohoku esophagus (TE)] with cisplatin (CDDP). We performed splinkerette PCR and TOPO cloning on the resistant colonies. Bacterial colonies were sequenced, and next-generation sequencing was used to identify the overexpressed/downregulated sequences as candidate genes for CDDP resistance. We established 4 cell lines of transposon-tagged cells, TE4, 5, 9 and 15. We treated the two relatively viable cell lines, TE4 and TE15, with CDDP. We identified 37 candidate genes from 8 resistant colonies. Eight genes were overexpressed whilst 29 were downregulated. Among these genes was Janus kinase 2 (JAK2) that is implicated in the progression of myeloproliferative neoplasms. We identified 37 candidate genes responsible for CDDP resistance in the two cell lines derived from ESCC cells. The method is inexpensive, relatively simple, and capable of introducing activating and de-activating mutations in the genome, allowing for drug-resistant genes to be identified.

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Clinical efficacy of drug-eluting stents in patients with diabetes

Cited by 2 publications

References 11 publications

Effects of undenatured whey protein supplementation on CXCL12- and CCL21-mediated B and T cell chemotaxis in diabetic mice

Effects of undenatured whey protein supplementation on CXCL12- and CCL21-mediated B and T cell chemotaxis in diabetic mice

Comprehensive screening of genes resistant to an anticancer drug in esophageal squamous cell carcinoma

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