Clinical Effectiveness of<i>Tyrophagus putrescentiae</i>Allergy by Local Nasal Immunotherapy Using Strips of<i>Dermatophagoides pteronyssinus</i>

Liao, En-Chih; Tsai, Jaw‐Ji

doi:10.3109/02770903.2011.611560

Cited by 2 publications

(1 citation statement)

References 21 publications

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“… 9 Local nasal immunotherapy is a well-established procedure with Der p crude extract to down-regulate Der p2-triggered histamine release. 10 It is therefore interesting to investigate a possible direct effect of Der p2 on epithelial activation.…”

Section: Introductionmentioning

confidence: 99%

Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling

Yin

Liao

Chiu

et al. 2015

Allergy Asthma Immunol Res

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PurposeHouse-dust-mite (HDM) major allergen Der p2 shares homology and function with Toll-like receptor (TLR) signaling protein myeloid differentiation-2 (MD2) and may lead to airway inflammation. Should Der p2 be internalized by human airway epithelium, it has the theoretical propensity to potentiate epithelium activation. This study aimed to demonstrate the internalization of Der p2 by airway epithelium and to investigate the effects of Der p2 on MD2 expression and epithelium activation.MethodsInternalization of recombinant, enhanced green fluorescent protein-labelled Der p2 (rDer p2-EGFP) into human airway epithelium (BEAS-2B) was tracked by laser confocal microscopy and confirmed by immunoblotting. Reverse-transcription polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemical staining were used to determine the effect of Der p2 on MD2 expression in vitro and ex vivo. Expression of messenger RNA (mRNA) encoding receptors/cytokines was measured by RT-PCR. Secretion of interleukin-6/interleukin-8 (IL-6/IL-8) was measured by enzyme-linked immunosorbent assay (ELISA).ResultsInternalization of Der p2 by BEAS-2B was confirmed by confocal microscopy and immunoblotting using rDer p2-EGFP and rDer p2, respectively. Expression of MD2 protein was increased in BEAS-2B and human nasal polyp airway epithelium cultured with rDer p2. Recombinant Der p2-cultured BEAS-2B caused little spontaneous IL-6/IL-8 secretion but significantly augmented by TLR ligand LPS. IL-6 secretion was up-regulated after MD2 transfection. Internalization of Der p2 was reduced by TLR2 RNA knockdown. Dexamethasone, calcitriol, anti-MD2/anti-TLR2 antibodies, and signalling inhibitors significantly reduced LPS+Der p2-induced IL-6/IL-8 secretion.ConclusionsHuman airway epithelium may internalize Der p2, which potentiates the response to environmental proinflammatory stimuli through MD2 and TLRs. This study highlights a novel mechanism and alleviates IL-6/IL-8 secretion in mite-induced airway inflammation.

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Section: Introductionmentioning

confidence: 99%

Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling

Yin

Liao

Chiu

et al. 2015

Allergy Asthma Immunol Res

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Combining antigen and immunomodulators: Emerging trends in antigen-specific immunotherapy for autoimmunity

Northrup

Christopher

Sullivan

et al. 2016

Advanced Drug Delivery Reviews

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Clinical Effectiveness ofTyrophagus putrescentiaeAllergy by Local Nasal Immunotherapy Using Strips ofDermatophagoides pteronyssinus

Abstract: Our study demonstrated that not only was Tyr p2-specific IgE reduced in the sera, but also its biological activity of Tyr p2-triggered BHR after LNIT with Dp strips. It is conceivable that the clinical effectiveness of Tp allergy by LNIT with Dp allergen strips may exist.

Cited by 2 publications

References 21 publications

Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling

Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling

Combining antigen and immunomodulators: Emerging trends in antigen-specific immunotherapy for autoimmunity

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