Clinical correlates of red blood cell omega-3 fatty acid content in male veterans with peripheral arterial disease

Nosova, Emily; Chong, Karen; Alley, Hugh; Harris, William S.; Boscardin, W. John; Conte, Michael S.; Owens, Christopher D.; Grenon, S. Marlene

doi:10.1016/j.jvs.2014.05.040

Cited by 9 publications

(5 citation statements)

References 38 publications

(45 reference statements)

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“…A higher omega‐3 index is associated with older age, increasing body mass index, and a history of smoking and fish oil intake in patients with peripheral artery disease (Nosova et al. ). RBC n −6 fatty acids have been found to be positively associated, and RBC n −3 fatty acids are inversely associated with body weight gain (Wang et al.…”

Section: Discussionmentioning

confidence: 99%

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

et al. 2019

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Omega‐3 fatty acids have long been ascribed a positive cardiovascular function. However, the plasma measurements invariably ignore 40% of the blood specimen, cells that engage in continuous exchange with their environment. In our study, we included all components of the circulating blood. Erythrocyte or red‐blood‐cell ( RBC ) n −3 fatty acid status has been linked to cardiovascular disease and death. A low omega‐3 index is an independent risk factor for cardiovascular disease and mortality. We tested the hypothesis that acute, maximal exercise would influence the relationship between RBC and serum fatty acids. RBC fatty acids profiling was achieved using targeted HPLC ‐ MS mass spectrometry. Healthy volunteers performed maximal treadmill exercise testing using the modified Bruce protocol. Central hemodynamics were monitored and maximal workload was assessed in metabolic equivalents ( MET s). Venous blood was obtained for RBC lipidomics. With the incremental exercise test, no fatty acid‐level variations were found in RBC s, while heart rate and arterial blood pressure increased significantly. No changes occurred in the omega‐3 quotient, namely the percentage of eicosapentaenoic acid and docosahexaenoic acid in RBC fatty acids in the RBC membrane. Nonetheless, maximal (13.50 ± 1.97 MET s) exercise intensity led to a decrease of RBC lauric acid (C12:0) in the recovery period. These data suggest that despite significant hemodynamic effects, short‐term maximal exercise is insufficient to alter RBC n −3 and other fatty‐acid status, including the omega‐3 quotient, in healthy individuals. RBC lauric acid deserves further scrutiny as a potential regulator of cardiovascular and metabolic functions.

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Section: Discussionmentioning

confidence: 99%

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

et al. 2019

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“…It is currently unknown whether supplementation of n‐3 PUFAs could reduce neointimal hyperplasia after vascular intervention or surgery. It is noteworthy that recent studies suggest that short‐term high‐dose nutritional supplementation with fish oil can alter the lipid metabolome in subjects with peripheral artery disease, suggesting the basis for further interventional trials in this population (46). In summary, our study demonstrates that systemic administration of 2 DHA‐derived SPMs (RvD2 and MaR1) attenuates low flow‐induced arterial remodeling in mice, likely via effects on leukocyte trafficking, macrophage, and VSMC phenotype.…”

Section: Discussionmentioning

confidence: 99%

Systemic delivery of proresolving lipid mediators resolvin D ₂ and maresin 1 attenuates intimal hyperplasia in mice

et al. 2015

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Vascular injury induces a potent inflammatory response that influences vessel remodeling and patency, limiting long-term benefits of cardiovascular interventions such as angioplasty. Specialized proresolving lipid mediators (SPMs) derived from v-3 polyunsaturated fatty acids [eicosapentaenoic acid and docosahexaenoic acid (DHA)] orchestrate resolution in diverse settings of acute inflammation. We hypothesized that systemic administration of DHA-derived SPMs [resolvin D2 (RvD2) and maresin 1 (MaR1)] would influence vessel remodeling in a mouse model of arterial neointima formation (carotid ligation). In vitro, SPM treatment inhibited mouse aortic smooth muscle cell migration (IC 50 @ 1 nM) to a PDGF gradient and reduced TNF-a-stimulated p65 translocation, superoxide production, and proinflammatory gene expression (MCP-1). In vivo, adult FVB mice underwent unilateral carotid artery ligation with administration of RvD2, MaR1, or vehicle (100 ng by intraperitoneal injection at 0, 1, 3, 5, and 7 d after ligation). In ligated carotid arteries at 4 d, SPM treatment was associated with reduced cell proliferation and neutrophil and macrophage recruitment and increased polarization of M2 macrophages in the arterial wall. Neointimal hyperplasia (at 14 d) was notably attenuated in RvD2 (62%)-and MaR1 (67%)-treated mice, respectively. Modulation of resolution pathways may offer new opportunities to regulate the vascular injury response and promote vascular homeostasis.-Akagi, D., Chen, M., Toy, R., Chatterjee, A., Conte, M. S. Systemic delivery of proresolving lipid mediators resolvin D2 and maresin 1 attenuates intimal hyperplasia in mice. FASEB J. 29, 2504-2513 (2015). www.fasebj.org

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“…n-3 PUFAs derived from fish oil have been found to yield beneficial effects on cardiovascular health (25, 26), including decreasing mortality from cardiovascular causes, all-cause mortality, and non-fatal cardiovascular events (27–30). Our previous work has shown that predictors of higher omega-3 index in patients with PAD were older age, elevated BMI and prior fish oil supplementation (12). Here we investigated the predictors of a response to fish oil supplementation and found that a lower BMI and higher LDL cholesterol were the only independent predictors of a favorable response in the omega-3 index.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously reported that in patients with PAD, a higher omega-3 index was associated with older age, elevated body mass index (BMI), and prior fish oil supplementation, while a history of smoking correlated with a lower omega-3 index (12). We also demonstrated the ability of high-dose, short duration n-3 PUFA supplementation to increase the omega-3 index in patients with PAD (13).…”

Section: Introductionmentioning

confidence: 99%

Predictors of change in omega-3 index with fish oil supplementation in peripheral artery disease

Drudi

Schaller

Hiramoto

et al. 2017

Journal of Surgical Research

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Introduction The omega-3 index represents the red blood cell (RBC) content of two major long-chain n-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We sought to determine factors associated with a favorable response to fish oil treatment as well as to characterize changes in RBC PUFAs associated with fish oil supplementation. Methods This study was a secondary analysis of the OMEGA-PAD I Trial, a randomized, double-blinded, placebo-controlled trial investigating short-duration, high-dose n-3 PUFA oral supplementation on endothelial function and inflammation in subjects with peripheral arterial disease (PAD). Patients with mild to severe claudication received either 4.4g of fish oil providing 2.6g of EPA + 1.8g of DHA daily (n=40) or placebo capsules (n=40) for 1 month. The RBC fatty acid content was measured by gas chromatography and expressed as a percent of total fatty acids. The change in omega-3 index was calculated as the difference between pre- and post-supplementation in the fish oil and placebo groups. Univariate analysis identified predictors of change in omega-3 index, with these variables included in our multivariable model. Results In the fish oil group, there was an increase in the omega-3 index (5.1 ± 1.3% to 9.0 ± 1.8%; p<0.0001), whereas there was no change in the control group. Factors associated with a favorable response (i.e., greater than the median change of 4.06%) included a lower body mass index (BMI) and higher concentrations of low-density lipoproteins (LDL). Other demographic/lifestyle factors such as age, race, or smoking status were unrelated to the response. Oral n-3 PUFA supplementation also decreased the n-6 PUFA content in RBCs. Conclusion Short-term, high-dose n-3 PUFA supplementation increases the omega-3 index to a greater extent in patients with a lower BMI and higher total and LDL cholesterol levels.

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Clinical correlates of red blood cell omega-3 fatty acid content in male veterans with peripheral arterial disease

Cited by 9 publications

References 38 publications

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

Systemic delivery of proresolving lipid mediators resolvin D ₂ and maresin 1 attenuates intimal hyperplasia in mice

Predictors of change in omega-3 index with fish oil supplementation in peripheral artery disease

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Clinical correlates of red blood cell omega-3 fatty acid content in male veterans with peripheral arterial disease

Cited by 9 publications

References 38 publications

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

Maximal exercise and erythrocyte fatty‐acid status: alipidomicsstudy

Systemic delivery of proresolving lipid mediators resolvin D 2 and maresin 1 attenuates intimal hyperplasia in mice

Predictors of change in omega-3 index with fish oil supplementation in peripheral artery disease

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Systemic delivery of proresolving lipid mediators resolvin D ₂ and maresin 1 attenuates intimal hyperplasia in mice