Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial

Olms, Constanze; Hix, Nathalie; Neumann, Heinrich; Yahiaoui-Doktor, Maryam; Remmerbach, Torsten Wilhelm

doi:10.1186/s13005-018-0166-4

Cited by 16 publications

(22 citation statements)

References 30 publications

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“…Exfoliative cytology is the microscopic examination of shed or desquamated cells from the mucus membrane, and it is a simple, safe, and reliable approach. Exfoliative cytology consists of the conventional method and the liquid-based cytology (LBC) method [5]. The conventional method involves chair side scraping of the oral mucosa and then smearing it directly onto a glass slide.…”

Section: Introductionmentioning

confidence: 99%

Clinical study on primary screening of oral cancer and precancerous lesions by oral cytology

et al. 2020

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Background This study was conducted to compare the histological diagnostic accuracy of conventional oral-based cytology and liquid-based cytology (LBC) methods. Methods Histological diagnoses of 251 cases were classified as negative (no malignancy lesion, inflammation, or mild/moderate dysplasia) and positive [severe dysplasia/carcinoma in situ (CIS) and squamous cell carcinoma (SCC)]. Cytological diagnoses were classified as negative for intraepithelial lesion or malignancy (NILM), oral low-grade squamous intraepithelial lesion (OLSIL), oral high-grade squamous intraepithelial lesion (OHSIL), or SCC. Cytological diagnostic results were compared with histology results. Results Of NILM cytology cases, the most frequent case was negative [LBC n = 50 (90.9%), conventional n = 22 (95.7%)]. Among OLSIL cytodiagnoses, the most common was negative (LBC n = 34; 75.6%, conventional n = 14; 70.0%). Among OHSIL cytodiagnoses (LBC n = 51, conventional n = 23), SCC was the most frequent (LBC n = 31; 60.8%, conventional n = 7; 30.4%). Negative cases were common (LBC n = 13; 25.5%, conventional n = 14; 60.9%). Among SCC cytodiagnoses SCC was the most common (LBC n = 16; 88.9%, conventional n = 14; 87.5%). Regarding the diagnostic results of cytology, assuming OHSIL and SCC as cytologically positive, the LBC method/conventional method showed a sensitivity of 79.4%/76.7%, specificity of 85.1%/69.2%, false-positive rate of 14.9%/30.7%, and false-negative rate of 20.6%/23.3%. Conclusions LBC method was superior to conventional cytodiagnosis methods. It was especially superior for OLSIL and OHSIL. Because of the false-positive and false-negative cytodiagnoses, it is necessary to make a comprehensive diagnosis considering the clinical findings.

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Section: Introductionmentioning

confidence: 99%

Clinical study on primary screening of oral cancer and precancerous lesions by oral cytology

et al. 2020

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“…The use of a cytology brush yields cells from deeper layers of the epithelium 16 . LBC has improved the quality and quantity of conventional cytology since it increases the number of cells dispersed in a homogenous thin layer with a clear background 10 . Therefore, it enhances sensitivity and quality of the specimen 16 .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it reduces the problem related to sampling in the preparation of smears, it reduces false negative rates; and therefore, it improves the sensitivity and quality of the cytology 6,7 . However, the lack of studies reporting oral LBC‐standardized protocols, and the elevated cost of materials and methodologies, challenges its adoption in the daily clinical practice and research 5,8‐13 . There is a need of having a low cost, reproducible, and easily accessible protocol; available worldwide that could be implemented for the cellular and molecular study of several oral mucosa lesions, so that cytopathologists could have an additional and complementary diagnostic tool, with a considerable potential for their early detection.…”

Section: Introductionmentioning

confidence: 99%

Optimized liquid‐based cytology for the cellular and molecular analysis of oral keratinocytes: A promising diagnostic tool

Osorio-Osorno

Arboleda-Toro

Arango

et al. 2020

Diagnostic Cytopathology

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Background Liquid‐based cytology (LBC) has improved exfoliative cytology by facilitating the extraction of more precise information from epithelial cells. The aim of this study was to optimize a protocol using a conventional cytobrush to perform LBC, obtaining oral keratinocytes for their further cellular and molecular analysis. Methods LBC was performed in 30 healthy donors from buccal mucosa. We evaluated the use of diethyl pyrocarbonate (DEPC)‐treated Dulbecco's Modified Eagle Medium (DMEM) medium right after the collection of the cells. Cell morphology and viability were determined by Orcein staining and flow cytometry, respectively. RNA was extracted by the trizol method, and evaluated with spectrometry and electrophoresis. Finally, RNA was copied into cDNA and GAPDH and TLR2 genes were amplified by reverse transcription polymerase chain reaction (RT‐PCR) and quantitative reverse transcription polymerase chain reaction (RT‐qPCR) using specific primers. Results Only DEPC‐treated DMEM preserved the viability of intact intraepithelial keratinocytes. RNA quantity and quality improves in samples treated with DEPC. RNA integrity is comparable with a cell line control. GAPDH gene was successfully amplified by RT‐PCR and RT‐qPCR. Conclusions Therefore, LBC performed under these conditions becomes a reproducible technique for the retrieval of intraepithelial oral keratinocytes with good cell viability for cytomorphometric analysis, and extraction of good RNA quality suitable for molecular analyses such as PCR. We propose this LBC protocol as a complementary method to the cellular and molecular study of oral mucosa pathologies; however, it requires further study.

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“…Liquid-based cytology was chosen, whose advantages are described in detail by Olms et al [ 41 ]. In order to obtain sufficient cell material, two brushes per sampling site were used [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Colonization and Tissue Compatibility of Denture Base Resins

et al. 2018

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Currently, there is minimal clinical data regarding biofilm composition on the surface of denture bases and the clinical tissue compatibility. Therefore, the aim of this experimental study was to compare the bacterial colonization and the tissue compatibility of a hypoallergenic polyamide with a frequently used PMMA resin tested intraorally in a randomized split-mouth design. Test specimens made of polyamide (n = 10) and PMMA (n = 10) were attached over a molar band appliance in oral cavity of 10 subjects. A cytological smear test was done from palatal mucosa at baseline and after four weeks. The monolayers were inspected for micronuclei. After four weeks in situ, the appliance was removed. The test specimens were immediately cultivated on non-selective and selective nutrient media. All growing colonies were identified using VITEK-MS. The anonymized results were analyzed descriptively. A total of 110 different bacterial species could be isolated, including putative pathogens. An average of 17.8 different bacterial species grew on the PMMA specimens, and 17.3 on the polyamide specimens. The highest number of different bacterial species was n = 24, found on a PMMA specimen. On the two specimens, a similar bacterial distribution was observed. Micronuclei, as a marker for genotoxic potential of dental materials, were not detected. This study indicates that the composition of bacterial biofilm developed on these resins after four weeks is not influenced by the type of resin itself. The two materials showed no cytological differences. This investigation suggests that polyamide and PMMA are suitable for clinical use as denture base material.

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Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial

Cited by 16 publications

References 30 publications

Clinical study on primary screening of oral cancer and precancerous lesions by oral cytology

Clinical study on primary screening of oral cancer and precancerous lesions by oral cytology

Optimized liquid‐based cytology for the cellular and molecular analysis of oral keratinocytes: A promising diagnostic tool

Bacterial Colonization and Tissue Compatibility of Denture Base Resins

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