Clinical chemistry and haematology historical data in control Sprague-Dawley rats from pre-clinical toxicity studies

Petterino, Claudio; Argentino-Storino, Alberta

doi:10.1016/j.etp.2005.10.002

Cited by 210 publications

(137 citation statements)

References 2 publications

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“…Though not statistically different, ALP and subsidiary AST values were slightly lower in operated rats when compared with untreated animals, despite a fairly good agreement with control data intervals on fasted rats reported in the literature. 18 Postoperative variations in clinical biochemistry parameters were described by van Wijk et al 11 who noted them to be outside normal limits in rats following surgery. Also, Faure et al 13 reported enzyme and total bilirubin parameters outside the normal range on day two in 15 -65% of rats following surgery, but approximately half of these animals regained normal values eight days postsurgery.…”

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confidence: 99%

An updated and simplified method for bile duct cannulation of rats

et al. 2010

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The single bile duct cannulated rat model was used for short-term intestinal absorption studies. Rats were randomly assigned to three groups: (A) bile duct cannulated, (B) control laparotomy and (C) no treatment. The body weight and health of the rats were monitored before and until day 5 after surgery, while bile flow was measured in group A on day 2. On the fifth day, tail vein blood was harvested, and alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin were quantified. Analysis of variance showed no significant difference between the groups for these parameters. This study demonstrated the suitability of a surgical rat model feasible for evaluation of the impact of bile in pharmacokinetic studies.

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confidence: 99%

An updated and simplified method for bile duct cannulation of rats

et al. 2010

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“…Although some sporadic differences were observed, these differences were not considered to be related to toxicity because the observed changes were within the normal reference laboratory range (Petterino et al, 2006). Serum chemistry results showed that extracts of Aster tataricus L. f., especially petroleum ether extract, affect liver function and have some effect on the heart.…”

Section: Histopathologymentioning

confidence: 93%

EVALUATION OF THE ACUTE AND SUBCHRONIC TOXICITY OF Aster tataricus L. F.

Peng¹,

Xin²,

Luo³

et al. 2016

Afr J Tradit Complement Altern Med

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Background: Aster tataricus L. f. is used as a traditional Chinese drug to relieve cough and asthma symptoms and to eliminate phlegm. However, Aster tataricus L. f. possesses toxicity, and little systematic research has been conducted on its toxic effects in the laboratory. Methods and Materials:The acute group was administered 75% alcohol extract of Aster tataricus L. f. in a single dose. A subchronic toxicity study was performed via daily oral administration of Aster tataricus L. f. at a dose of 0.34 g/kg body weight in SD rats. The rats were divided into six groups: a petroleum ether extract (PEA) group, an ethyl acetate extract (EEA) group, an n-butyl alcohol extract (NEA) group, a remaining lower aqueous phases (REA) group, a 75% alcohol extract (AEA) group and a control group. Quantitative measurements of cytokines were obtained by fluorescence with a laser scanner using a Cy3 equivalent dye. Results:The LD 50 of the 75% alcohol extract of Aster tataricus L. f. was 15.74 g/kg bw. In the subchronic toxicity study, no significant differences were observed among groups in relative organ weights, urine traits, liver antioxidase levels, or cytokine levels.However, significant sporadic differences were observed in body weight gains, haematology indices, biochemistry values, and histopathology features in PEA, EEA group. In addition, sporadic changes in other groups in measures such as WBC, MCHC, CK, ALP, AST, ALT, LDH, T-BIL, LDL-C, HDL-C, and TC were observed. Conclusion:The toxicity study showed that Aster tataricus L. f. can produce toxic effects, mainly on the liver; much less on the heart. The LD 50 was 15.74 g/kg BW in mice, and the subchronic toxicity study, used a dosage of 0.34 g/kg/d.BW, showed that the toxic components of Aster tataricus L. f. were mainly concentrated in the petroleum ether fraction, followed by the ethyl acetate fraction, the n-butyl alcohol fraction, the lower aqueous phase and the 75% ethanol extracts.

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“…Additionally, neutrophil (NE) and percent of neutrophil (NEP) in the females of the 625 mg/kg/day group significantly increased, whereas the percent of lymphocyte (LYP) decreased significantly. However, the difference was slight and within the biological fluctuation range [26], with no dose-dependency. Thus, it was concluded that RCMLS did not influence it.…”

Section: Ophthalmic Examination Urinalysis Hematology and Serum Biomentioning

confidence: 99%

“…However, the range was very slight and within the biological change range [26]. Therefore, it was not considered toxicologically significant.…”

Section: Days Repeated Oral Dose Toxicity Studymentioning

confidence: 99%

Nutrition Composition and Single, 14-Day and 13-Week Repeated Oral Dose Toxicity Studies of the Leaves and Stems of Rubus coreanus Miquel

Song

Noh

et al. 2016

Molecules

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Abstract:The leaves and stems of the plant Rubus coreanus Miquel (RCMLS) are rich in vitamins, minerals and phytochemicals which have antioxidant, anti-hemolytic, anti-inflammatory, anti-fatigue and anti-cancer effects. However, RCMLS is not included in the Korean Food Standards Codex due to the lack of safety assurance concerning RCMLS. We evaluated single and repeated oral dose toxicity of RCMLS in Sprague-Dawley rats. RCMLS did not induce any significant toxicological changes in both male and female rats at a single doses of 2500 mg/kg/day. Repeated oral dose toxicity studies showed no adverse effects in clinical signs, body weight, food consumption, ophthalmic examination, urinalysis, hematology, serum biochemistry, necropsy findings, organ weight, and histopathology at doses of 625, 1250, and 2500 mg/kg/day. The LD 50 and LOAEL of RCMLS might be over 2500 mg/kg body weight/day and no target organs were identified. Therefore, this study revealed that single and repeated oral doses of RCMLS are safe.

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Clinical chemistry and haematology historical data in control Sprague-Dawley rats from pre-clinical toxicity studies

Cited by 210 publications

References 2 publications

An updated and simplified method for bile duct cannulation of rats

An updated and simplified method for bile duct cannulation of rats

EVALUATION OF THE ACUTE AND SUBCHRONIC TOXICITY OF Aster tataricus L. F.

Nutrition Composition and Single, 14-Day and 13-Week Repeated Oral Dose Toxicity Studies of the Leaves and Stems of Rubus coreanus Miquel

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