Clinical Characteristics and Results of Semen Tests Among Men With Coronavirus Disease 2019

Li, Diangeng; Jin, Meiling; Bao, Pengtao; Zhao, Weiguo; Zhang, Shixi

doi:10.1001/jamanetworkopen.2020.8292

Cited by 632 publications

(723 citation statements)

References 5 publications

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“…Correspondingly, extensive IgG precipitation was mainly 154 found in the interstitial compartments and partly in the seminiferous epithelium of COVID-19 155 patient testes, which is consistent with the effects of SARS infected testes ( we found that the two testis samples (patient-1 and 3) were positive for SARS-CoV-2 175 nuclear acid ( Table S2). The detection of the SARS-CoV-2 virus within the testis, a finding 176 consistent with other study (Li et al, 2020), suggests that direct viral effects, as well as the 177 induced damage of the testicular immune privilege, may contribute to the germ cell injury. 178…”

Section: Results and Discussion 115supporting

confidence: 78%

“…Since progressive respiratory failure is the primary 72 cause of death in the COVID-19 pandemic, researchers mainly focused on the 73 pathophysiological changes in major organs at autopsy, such as the lung, heart, and Kidney 74 have been cured, among them a large number of men who have fertility desire, which 78 raises concerns about whether SARS-CoV-2 could attack the testicular cells and might 79 cause male infertility. Two studies were unable to detect the presence of SARS-CoV-2 in 80 semen and testis from men who had recovered from COVID-19 (Pan et al, 2020;Song et 81 al., 2020), but in contrast, a recent study has reported that SARS-CoV-2 can be detected in 82 semen of patients with COVID-19 (Li et al, 2020). Therefore, it is currently unknown 83 whether the infection of SARS-CoV-2 will damage spermatogenesis and male fertility or 84 whether SARS-CoV-2 can attack the germ cell into seminiferous tubules.…”

Section: Healthmentioning

confidence: 99%

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Pathological and molecular examination findings in postmortem testis biopsies reveal spermatogenesis damages in COVID-19 patients

Guan

Chen

et al. 2020

Preprint

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Despite widespread interest in the pathophysiology of COVID-19 in respiratory and cardiovascular systems, little is known about the morphologic and molecular changes in the testis of patients with COVID-19 and the effects of SARS-CoV-2 infection on male fertility. We report here on the pathophysiology and molecular feature of testes obtained at autopsy from six men with COVID-19, as compared with those of testes from three men with age-matched, uninfected SARS-CoV-2. Our histopathological results showed that all COVID-19 patients had severe spermatogenesis damages compared with controls. Importantly, we detected the nuclear acid of the SARS-CoV-2 virus, viral particles, and SARS-CoV-2 spike S1 protein in COVID-19 patient testes, and we also found ACE2 and TMPRSS2 significantly elevated in the testes from COVID-19 patients. Furthermore, we observed a prominent leukocyte infiltration, including CD3+ T lymphocytes, CD20+ B lymphocytes, CD68+ macrophages, HLA-DR+ myeloid cells, and CD38+ plasma cells in the testes of COVID-19 patients. RNA-Seq analyses further revealed SARS-CoV-2 infection could lead to dysfunction of the genes that regulate the spermatogenesis and inflammation response-related pathways. Collectively, our pathological and molecular examination findings indicate that SARS-CoV-2 could directly attack testicular cells, thereby inducing the damage of testicular immune privilege and spermatogenesis defects.

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Section: Results and Discussion 115supporting

confidence: 78%

Section: Healthmentioning

confidence: 99%

Pathological and molecular examination findings in postmortem testis biopsies reveal spermatogenesis damages in COVID-19 patients

Guan

Chen

et al. 2020

Preprint

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“…While this finding suggested that the testis was a high-risk organ that was vulnerable to SARS-CoV-2 infection, direct entry of the virus into testis would be difficult due to the presence of the blood-testis barrier. One study stated that SARS-CoV-2 coronavirus was detected in the semen of male 19 . Despite this, orchitis was reported as a complication of SARS 35 .…”

Section: Discussionmentioning

confidence: 99%

“…Another retrospective, multi-center cohort study demonstrated the epidemiological and clinical characteristics of 191 COVID-19 patients, of whom 27 individuals were reported to have AKI, and ten were treated with continuous renal replacement therapy (CRRT) 9 . A brief review of existing papers that reported urology system impairment associated with COVID-19 infection is shown in Table 1 [8][9][10][11][12][13][14][15][16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Multiple expression assessments of ACE2 and TMPRSS2 SARS-CoV-2 entry molecules in the urinary tract and their associations with clinical manifestations of COVID-19

Ren

Wei

et al. 2020

Preprint

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Background: Since December 2019, the novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first spread quickly in Wuhan, China, then globally. From previously published evidence, ACE2 and TMPRSS2, are both pivotal entry molecules that enable cellular infection by SARS-CoV-2. Meanwhile, increased expression of pro-inflammatory cytokines, or a "cytokine storm," is associated with multiple organ dysfunction syndrome that is often observed in critically ill patients. Methods: We investigated the expression pattern of ACE2 and TMPRSS2 in major organs in the human body, especially under specific disease conditions. Multiple sequence alignment of ACE2 in different species was used to explain animal susceptibility. Moreover, the cell-specific expression patterns of ACE2 and cytokine receptors in the urinary tract were assessed using single-cell RNA sequencing (scRNA-seq). Additional biological relevance was determined through Gene Set Enrichment Analysis (GSEA) using an ACE2 specific signature. Results: Our results revealed that ACE2 and TMPRSS2 were highly expressed in genitourinary organs. ACE2 was highly and significantly expressed in the kidney among individuals with chronic kidney diseases or diabetic nephropathy. In single cells, ACE2 was primarily enriched in gametocytes in the testis, and renal proximal tubules. The receptors for pro-inflammatory cytokines, especially IL6ST, were remarkably concentrated in endothelial cells, macrophages, and spermatogonial stem cells in the testis, and renal endothelial cells, which suggested the occurrence of alternative damaging mechanisms via autoimmune attacks. Conclusions: This study provided new insights into the pathogenicity mechanisms of SARS-CoV-2 that underlie the clinical manifestations observed in the human testis and kidney. These observations might substantially facilitate the development of effective treatments for this rapidly spreading disease.

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“…Neben der Schleimhaut des Nasen-Rachenraums lässt sich das SARS-CoV-2-RNA auch in verschiedenen anderen Körperflüssigkeiten z. B. in Stuhl [45], Liquor [47], Samen [48] und Muttermilch [49] finden.…”

Section: Andere Körperflüssigkeitenunclassified

Labordiagnostik bei SARS-CoV-2-Infektionen

Roskos

2020

Der Klinikarzt

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ZUSAMMENFASSUNGDie aktuelle COVID-19-Pandemie stellte auch die medizinischen Labore vor große Herausforderungen. Der prinzipiell sehr frühen Kenntnis des primär neuen Erregers und der prinzipiellen Möglichkeit des Nachweises des Virus stand oft eine zunächst unzureichende Verfügbarkeit an Reagenzien und Analysensystemen gegenüber, die aber relativ schnell verbessert werden konnten, sodass nun eine flächendeckende und schnelle Diagnostik dieses Erregers möglich ist. Neben der Erregeridentifizierung liefert die Labordiagnostik jedoch auch wichtige Hinweise bei der Beurteilung der Erkrankung bzw. der Abschätzung des weiteren Verlaufes. Die Wertigkeit einzelner Parameter bzw. Parameterkonstellationen wurde dabei teilweise erst im Verlauf der Pandemie deutlich bzw. ist auch noch in der Evaluierung.

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Clinical Characteristics and Results of Semen Tests Among Men With Coronavirus Disease 2019

Cited by 632 publications

References 5 publications

Pathological and molecular examination findings in postmortem testis biopsies reveal spermatogenesis damages in COVID-19 patients

Pathological and molecular examination findings in postmortem testis biopsies reveal spermatogenesis damages in COVID-19 patients

Multiple expression assessments of ACE2 and TMPRSS2 SARS-CoV-2 entry molecules in the urinary tract and their associations with clinical manifestations of COVID-19

Labordiagnostik bei SARS-CoV-2-Infektionen

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