Clinical case of cfr-positive MRSA CC398 in Belgium

Paridaens, Henry; Coussement, Julien; Argudín, Maria Ángeles; Delaere, Bénédicte; Huang, Te-Din; Glupczynski, Youri; Denis, Olivier

doi:10.1007/s10096-017-2953-4

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…isolates recovered from livestock and the livestock environment (Li et al 2017;Guo et al 2018;Chanchaithong et al 2019;Ruiz-Ripa et al 2020b), which is likely due to the co-selection by the extended use of other antimicrobial agents, such as florfenicol. Linezolid-resistant MRSA isolates of the CC398 carrying the cfr gene have been sporadically reported in different countries, mainly colonizing pigs (Kehrenberg et al 2009;Peeters et al 2015) but also colonizing and infecting humans (Paridaens et al 2017;Ruiz-Ripa et al 2020a). The detection of this multiresistance gene among LA-MRSA CC398 is of great concern not only for veterinary medicine, but also for humans in close contact since the transmission of this genetic lineage from pigs to humans has been reported (Price et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…LA-MRSA comprises different well-known genetic lineages with a certain geographical distribution, being the clonal complex CC398 the predominant in Europe and North America, and the CC9 highly prevalent in Asian countries (Ye et al 2016;Guo et al 2018). LA-MRSA is mainly related to colonization in pigs and humans with professional exposure to livestock (Ye et al 2016;Chanchaithong et al 2019;Ruiz-Ripa et al 2020a); however, it has also been detected causing infection either in animals or humans (Butaye et al 2016;Paridaens et al 2017;Santos et al 2020). Moreover, LA-MRSA has also been recovered from meat products, increasing the concern about the possible transmission of these genetic lineages via food consumption or handling (Wu et al 2018;Mama et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

et al. 2021

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Aims: To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018-2019. Methods and Results: The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular techniques. The transferability of the cfr gene was assessed by conjugation and its genetic environment was determined by PCR mapping. In all, 21 isolates were methicillin-resistant S. aureus (MRSA) carrying the mecA gene (SCCmecV or non-typeable SCCmec), whereas the remaining two were methicillin-susceptible (MSSA). All but one MRSA isolates (n = 20) belonged to the CC398, being the spa t011 the most prevalent (n = 11). The remaining MRSA and the two MSSA isolates were ascribed to ST9/CC9. The S. aureus isolates exhibited resistance to (number of resistant isolates): b-lactamics (21), erythromycin and/or clindamycin (20), aminoglycosides (7), tetracycline (22), fluoroquinolones (14), chloramphenicol (5) and linezolid (1). The S. aureus isolates did not carry any of the virulence genes studied. One MRSA belonging to the CC398 showed linezolid resistance mediated by the cfr gene. The cfr gene was co-located with fexA in the Tn558 variant previously reported in the S. aureus plasmid pSCFS7. Conclusions: Two major livestock-associated genetic lineages were detected among pigs with signs of infection in Spain. The presence of the cfr gene among LA-MRSA-CC398 is of great concern not only for veterinary medicine, but also for humans in close contact. Significance and Impact of the Study: This work describes the molecular characterization of S. aureus isolates recovered from pigs with signs of infection and we report, as far as we know, the first description of MRSA-CC9 from pigs in Spain. Moreover, the detection of a MRSA-CC398 isolate carrying the multiresistance cfr gene highlights the need for continuous surveillance and awareness of LA-MRSA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

et al. 2021

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“…Recently, a clinical case due to a cfr -positive livestock-associated (LA-) MRSA CC398 has been described [119]. This gene has been found in important MRSA pandemic lineages such as the sequence type (ST)22/SCC mec IV, the Panton-Valentine leukocidin (PVL) positive ST8/SCC mec IV/USA300 or the ST125-MRSA-IVc [114,120,121].…”

Section: Amr-genes In Gram-positive Bacteria From Animalsmentioning

confidence: 99%

“…Studies based on the phylogenetic analysis of genome-wide single nucleotide polymorphisms (SNPs) supported the existence of two subpopulations in CC398: an ancestral human-adapted clade and an animal-associated clade [144,145]. Nevertheless, LA-MRSA CC398 isolates of the animal-associated clade are able to infect humans [119,145]. Analysis of genes present in CC398 has revealed numerous genes in common with clinical S. aureus and/or other staphylococci, but also some novel or rare resistance genes have been found in animal-related isolates from this lineage [79,81].…”

Section: Amr-genes In Gram-positive Bacteria From Animalsmentioning

confidence: 99%

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

et al. 2017

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Antimicrobial agents are used in both veterinary and human medicine. The intensive use of antimicrobials in animals may promote the fixation of antimicrobial resistance genes in bacteria, which may be zoonotic or capable to transfer these genes to human-adapted pathogens or to human gut microbiota via direct contact, food or the environment. This review summarizes the current knowledge of the use of antimicrobial agents in animal health and explores the role of bacteria from animals as a pool of antimicrobial resistance genes for human bacteria. This review focused in relevant examples within the ESC(K)APE (Enterococcus faecium, Staphylococcus aureus, Clostridium difficile (Klebsiella pneumoniae), Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae) group of bacterial pathogens that are the leading cause of nosocomial infections throughout the world.

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“… 1 The first report of linezolid resistance was in a staphylococci clinical isolate in 2001. 2 Since this time, linezolid-resistant MRSA 3 – 6 and the linezolid-resistant CoNS strains 7 – 8 have been increasingly isolated from the health care setting.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of cfr-mediated linezolid resistance among Staphylococcus species isolated from a teaching hospital in Beijing, China

et al. 2018

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ObjectiveThe aim of the present study was to report the dissemination of cfr and fexA genes mediated by linezolid resistance among Staphylococcus species.MethodsThree methicillin-resistant staphylococci that were collected from a teaching hospital in Beijing were identified as linezolid-resistant. These three staphylococci were Staphylococcus aureus, S. haemolyticus, and S. cohnii. Mutations in domain V of 23S ribosomal RNA, ribosomal proteins, and the cfr, fexA, and optrA genes were analysed.ResultsThe three isolates had no mutations of 23S ribosomal RNA, but showed mutations in the cfr and fexA genes. Mutations in the gene for ribosomal protein L3, which resulted in the amino acid exchanges Gly108Glu, Ser158Phe, and Asp159Tyr, were identified in S. cohnii X4535.ConclusionsThis is the first report of the cfr gene in clinical linezolid-resistant methicillin-resistant S. aureus isolated from Beijing. L3 mutations coupled with the cfr and fexA genes may act synergistically. Potential transmissibility of this agent, even without prior exposure to linezolid, may have serious epidemiological repercussions.

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Clinical case of cfr-positive MRSA CC398 in Belgium

Cited by 10 publications

References 12 publications

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

Dissemination of cfr-mediated linezolid resistance among Staphylococcus species isolated from a teaching hospital in Beijing, China

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