Clinical biochemistry of lactate dehydrogenase

Skillen, A. W.

doi:10.1002/cbf.290020304

Cited by 14 publications

(12 citation statements)

References 88 publications

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“…Therefore, LDH in serum reflects defects or disruptions of plasma membranes. (37,38) LDH readily leaves cells after any type of injury, and levels in culture medium are used to monitor cell and tissue damage. (37,38) Acute diseases typically generate much higher serum LDH concentrations than chronic disorders.…”

Section: Discussionmentioning

confidence: 99%

“…(37,38) LDH readily leaves cells after any type of injury, and levels in culture medium are used to monitor cell and tissue damage. (37,38) Acute diseases typically generate much higher serum LDH concentrations than chronic disorders. (37) Accordingly, elevated serum LDH is a classic marker for acute myocardial infarction.…”

Section: Discussionmentioning

confidence: 99%

“…Few drugs directly affect serum levels. (34)(35)(36)(37)(38)(39) Most serum LDH derives from the breakdown of erythrocytes and platelets, with varying contributions from other tissues, including liver, heart, and skeletal muscle. (41) Accordingly, quantitation of serum total LDH is a highly nonspecific test, and supranormal values do not implicate any particular organ.…”

Section: Discussionmentioning

confidence: 99%

“…During cell injury, the pattern profiles the compromised tissue. (37,42) (38,43) To diagnose myocardial infarction, elevation in LDH-1 is most useful. Mature erythrocytes feature LDH-1, -2, and -3, whereas immature erythrocytes have more LDH-5.…”

Section: Discussionmentioning

confidence: 99%

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Elevated serum lactate dehydrogenase isoenzymes and aspartate transaminase distinguish Albers-Schönberg disease (Chloride Channel 7 Deficiency Osteopetrosis) among the sclerosing bone disorders

Whyte

Kempa

McAlister

et al. 2010

Journal of Bone and Mineral Research

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Osteopetrosis (OPT) refers to the consequences of generalized failure of skeletal resorption during growth. Most cases are explained by loss-of-function mutation within the genes that encode either chloride channel 7 (CLCN7) or a vacuolar proton pump subunit (TCIRG1), each compromising acid secretion by osteoclasts. Patients suffer fractures and sometimes cranial nerve entrapment and insufficient medullary space for hematopoiesis. In 1996, we reported that a high serum level of the brain isoenzyme of creatine kinase (BB-CK), the CK of osteoclasts, characterizes OPT dueamong the sclerosing bone disorders (J Clin Endocrinol Metab. 1996;11:1438). Now, we show that elevation in serum of multiple lactate dehydrogenase (LDH) isoenzymes with aspartate transaminase (AST) distinguishes autosomal dominant OPT due to loss-of-function mutation in CLCN7 [Albers-Schö nberg disease (A-SD)] among these conditions. Serum total LDH and AST levels as high as 3Â and 2Â, respectively, the upper limits of normal for age-appropriate controls, were persistent and essentially concordant in A-SD. Serum LDH was elevated in 7 of 9 children and in the 2 adults studied with A-SD. LDH isoenzyme quantitation showed excesses of LDH-2, -3, and -4. Neither total LDH nor AST increases were found in other forms of OPT, including bisphosphonateinduced OPT, or in 41 children and 6 adults representing 20 additional sclerosing bone disorders. Serum TRACP-5b and BB-CK also were markedly elevated in A-SD. Hence, high serum levels of several enzymes characterize A-SD. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. ß

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Elevated serum lactate dehydrogenase isoenzymes and aspartate transaminase distinguish Albers-Schönberg disease (Chloride Channel 7 Deficiency Osteopetrosis) among the sclerosing bone disorders

Whyte

Kempa

McAlister

et al. 2010

Journal of Bone and Mineral Research

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“…2B,C). Cryoinjury was further validated using two independent cell death assays for lactate dehydrogenase (LDH) and cardiac troponin I (cTnI) release, which are both used clinically to detect suspected myocardial damage (Thygesen et al, 2012;Skillen, 1984). Cryoinjury in HES3 and HES3 NKX2-5 eGFP/w hCOs resulted in an approximately threefold increase in LDH release into the media compared with uninjured controls (Fig.…”

Section: Cryoinjury Induces Cell Death and Tissue Ablation In Hcosmentioning

confidence: 99%

Development of a human cardiac organoid injury model reveals innate regenerative potential

et al. 2017

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The adult human heart possesses a limited regenerative potential following an ischemic event, and undergoes a number of pathological changes in response to injury. While cardiac regeneration has been documented in zebrafish and neonatal mouse hearts, it is currently unknown whether the immature human heart is capable of undergoing complete regeneration. Combined progress in pluripotent stem cell differentiation and tissue engineering has facilitated the development of human cardiac organoids (hCO), which resemble fetal heart tissue and can be used to address this important knowledge gap. This study aimed to characterise the regenerative capacity of immature human heart tissue in response to injury. Following cryoinjury with a dry ice probe, hCO exhibited an endogenous regenerative response with full functional recovery by two weeks following acute injury. Cardiac functional recovery occurred in the absence of pathological fibrosis or cardiomyocyte hypertrophy. Consistent with regenerative organisms and neonatal human hearts, there was a high basal level of cardiomyocyte proliferation, which may be responsible for the regenerative capacity of the hCO. This study suggests that immature human heart tissue has an intrinsic capacity to regenerate.

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Development and validation of a simple‐to‐use nomogram for predicting refractoryMycoplasma pneumoniaepneumonia in children

Cheng

Lin

Zheng

et al. 2020

Pediatric Pulmonology

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Objective This study aimed to develop and validate a simple‐to‐use nomogram for predicting refractory Mycoplasma pneumoniae pneumonia (RMPP) in children. Methods A total of 73 children with RMPP and 146 children with general Mycoplasma pneumoniae pneumonia were included. Clinical, laboratory, and radiological data were obtained. A least absolute shrinkage and selection operator (LASSO) regression model was used to determine optimal predictors. The nomogram was plotted by multivariable logistic regression. The performance of the nomogram was assessed by calibration, discrimination, and clinical utility. Results The LASSO regression analysis identified lactate dehydrogenase, albumin, neutrophil ratio, and high fever as significant predictors of RMPP. This nomogram‐illustrated model showed good discrimination, calibration, and clinical value. The area under the receiver operating characteristic curve of the nomogram was 0.884 (95% CI, 0.823‐0.945) in the training set and 0.881 (95% CI, 0.807‐0.955) in the validating set. Calibration curve and Hosmer‐Lemeshow test showed good consistency between the predictions of the nomogram and the actual observations, and decision curve analysis showed that the nomogram was clinically useful. Conclusion A simple‐to‐use nomogram for predicting RMPP in early stage was developed and validated. This may help physicians recognize RMPP earlier.

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Clinical biochemistry of lactate dehydrogenase

Cited by 14 publications

References 88 publications

Elevated serum lactate dehydrogenase isoenzymes and aspartate transaminase distinguish Albers-Schönberg disease (Chloride Channel 7 Deficiency Osteopetrosis) among the sclerosing bone disorders

Elevated serum lactate dehydrogenase isoenzymes and aspartate transaminase distinguish Albers-Schönberg disease (Chloride Channel 7 Deficiency Osteopetrosis) among the sclerosing bone disorders

Development of a human cardiac organoid injury model reveals innate regenerative potential

Development and validation of a simple‐to‐use nomogram for predicting refractoryMycoplasma pneumoniaepneumonia in children

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