Clinical aspects of T‐ and B‐lymphocytes in rheumatic diseases

Messner, Ronald P.

doi:10.1002/art.1780170402

Cited by 33 publications

(16 citation statements)

References 27 publications

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“…This lymphopenia was accounted for by absolute decreases in SIg, EAC, Fc, and E-rosetting cells; however, the percentages of these cells were not significantly different from normals. Previously, we, as well as others, had found a significant percentage decrease in T cells in SLE (23,37,38). However, our previously studied SLE patients had disease of much longer duration than the patients reported here, and disease duration and activity may play a role in the T lymphopenia.…”

Section: Discussioncontrasting

confidence: 43%

Phytohemagglutinin Response in Systemic Lupus Erythematosus

Utsinger¹,

Yount²

1977

J. Clin. Invest.

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Section: Discussioncontrasting

confidence: 43%

Phytohemagglutinin Response in Systemic Lupus Erythematosus

Utsinger¹,

Yount²

1977

J. Clin. Invest.

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“…[11][12][13] The technique of magnet-activated cell sorting ͑MACS͒ allows target cell separation to be carried out in parallel, providing rapid separation ͑ϳ10…”

mentioning

confidence: 99%

Computational design optimization for microfluidic magnetophoresis

2011

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Current macro-and microfluidic approaches for the isolation of mammalian cells are limited in both efficiency and purity. In order to design a robust platform for the enumeration of a target cell population, high collection efficiencies are required. Additionally, the ability to isolate pure populations with minimal biological perturbation and efficient off-chip recovery will enable subcellular analyses of these cells for applications in personalized medicine. Here, a rational design approach for a simple and efficient device that isolates target cell populations via magnetic tagging is presented. In this work, two magnetophoretic microfluidic device designs are described, with optimized dimensions and operating conditions determined from a force balance equation that considers two dominant and opposing driving forces exerted on a magnetic-particle-tagged cell, namely, magnetic and viscous drag. Quantitative design criteria for an electromagnetic field displacement-based approach are presented, wherein target cells labeled with commercial magnetic microparticles flowing in a central sample stream are shifted laterally into a collection stream. Furthermore, the final device design is constrained to fit on standard rectangular glass coverslip ͑60 ͑L͒ ϫ 24 ͑W͒ ϫ 0.15 ͑H͒ mm 3 ͒ to accommodate small sample volume and point-of-care design considerations. The anticipated performance of the device is examined via a parametric analysis of several key variables within the model. It is observed that minimal currents ͑Ͻ500 mA͒ are required to generate magnetic fields sufficient to separate cells from the sample streams flowing at rate as high as 7 ml/h, comparable to the performance of current state-of-the-art magnet-activated cell sorting systems currently used in clinical settings. Experimental validation of the presented model illustrates that a device designed according to the derived rational optimization can effectively isolate ͑ϳ100%͒ a magnetic-particle-tagged cell population from a homogeneous suspension even in a low abundance. Overall, this design analysis provides a rational basis to select the operating conditions, including chamber and wire geometry, flow rates, and applied currents, for a magnetic-microfluidic cell separation device.

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“…It is possible that these antibodies are indicators of exposure to an agent; moreover, they may have the same significance as a false positive serology that may precede the overt onset of disease in SLE patients (27). If family members have been exposed to a virus and have successfully handled the insult, a search for antibodies specific for this agent may be more fruitful in the consanguineous household contacts than in SLE patients who themselves may have developed a more complete loss of their immunologic control mechanisms (1,2,28).…”

Section: Resultsmentioning

confidence: 99%

Anti-nucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies.

DeHoratius¹,

Pillarisetty²,

Messner³

et al. 1975

J. Clin. Invest.

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A B S T R A C T Anti-RNA antibodies were found in 82% of 28 systemic lupus erythematosus (SLE) probands and in 16% of 124 of their family members. The incidence in 76 control family members was only 5%. In the SLE family members, the antibodies were found exclusively in 21% of the 94 close household contacts of the probands. The incidence of anti-native DNA (nDNA) antibodies was 68% for the SLE probands. The incidence of anti-nDNA antibodies in close household contacts of the probands was 6%, which was not significantly different from the 1% incidence found in control families. Lymphocytotoxic antibodies occurred in 57% of the SLE family members as a whole and in 68% of the close household contacts. In the SLE probands, lymphocytotoxic antibodies correlated with anti-singlestranded RNA (poly A) and anti-nDNA but not with anti-double-stranded RNA (poly A-poly U). On the other hand, lymphocytotoxic antibodies in the household contacts correlated with anti-double-stranded RNA (poly A-poly U) but not with anti-poly A or anti-nDNA. The anti-RNA antibodies were present in consanguineous household contacts but not in nonconsanguineous household contacts. These findings strengthen the hypothesis that both an environmental agent, possibly a virus, as well as the genetic response are important in the pathogenesis of SLE. Family members may therefore be a logical population in whom to search for specific antibodies to a viral agent.

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Clinical aspects of T‐ and B‐lymphocytes in rheumatic diseases

Cited by 33 publications

References 27 publications

Phytohemagglutinin Response in Systemic Lupus Erythematosus

Phytohemagglutinin Response in Systemic Lupus Erythematosus

Computational design optimization for microfluidic magnetophoresis

Anti-nucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies.

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