Clinical Applications of Biological Dosimetry in Patients Exposed to Low Dose Radiation Due to Radiological, Imaging or Nuclear Medicine Procedures

Vinnikov, Volodymyr; Belyakov, O.V.

doi:10.1053/j.semnuclmed.2021.11.008

Cited by 4 publications

(5 citation statements)

References 219 publications

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“…In response to the problem of rapid, accurate and high‐throughput estimation of personal high biological dose exposure during nuclear emergencies and nuclear terrorism, we will focus on the research to solve the bottleneck of large quantities of biological dose estimation problem in large‐scale radiation emergencies. Establishing a unified standard curve of biological dose is urgently needed 8‐12 …”

Section: Discussionmentioning

confidence: 99%

“…To provide psychological counseling to people who are not radiation exposure, medical observation should be carried out for those below 0.1 Gy. For larger doses, medical treatment is carried out according to the principles of treatment at each dose level, it can guide the scientific and reasonable clinical medical treatment 8,22 …”

Section: Discussionmentioning

confidence: 99%

“…Establishing a unified standard curve of biological dose is urgently needed. [8][9][10][11][12] To address the above deficiencies, in this study revised and complemented the officially recommended conventional approach to such problems so that it can successfully deal with such incidents, individual differences in radiation sensitivity were studied, and the selection of donor volunteers to establish the biological dose curve was defined, the influence of radiation dose rate on the curve was quantitatively studied.…”

Section: Discussionmentioning

confidence: 99%

“…8,22 Another strategy to improve biodosimetric throughput is the development of a network of biodosimetric laboratories. A number of networks have been established to improve dose estimation throughput, such as the National Biological Dose Response Program (NBDRP) in Canada, the Biological dosimetry Network in Latin America and the Chromosome network in Japan.…”

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confidence: 99%

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Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Liu

2022

The FASEB Journal

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In order to achieve the goal of rapid response, effective controland protection of life inlarge-scale radiation events, the IAEA Manual 2011 has been revised based on the data of radio-sensitivity, dose-rate findings. Analyze individual differences in radiation sensitivity using 60 Co radiation (0.27 Gy/min). Chromosomal aberrations with different irradiation dose rates were used to establish the biological dose curve and analyze the excess of the "dicentric + ring" caused by the dose rate at each dose point; DAPI-images and Metafer 4 were used to capture metaphase images and make further analysis. The data were collected in 2020, Dicentric + ring/100 Cells was 17.5-43.8, the average value was28.32 ± 6.98. The mean value of Dicentric + ring/100 Cells was 31.37 in males while 25.27 in females, there are significant differences (p < .01). The irradiation dose is dominant, At each dose point, the value of"(dicentric chromosome + centric rings)/cell" is proportional to "dose rate", that is, Y = kx + b, within the dose range of 1-5 Gy, "(dicentric chromosome + centric rings)/ Cell" holds a quadratic linear relationship with dose rate, that is, y = ax 2 + bx + c; The DAPI-images might give you more hints than those of conventional Giemsa-stain.The authors recommend that the IAEA Manual 2011 could be revised based on data of radio-sensitivity and dose-rate, which may contribute to the establishment of a unified dose-response calibration curve and stimulation of potential for automation in cytogenetic biodosimetry. (1) Individual differences of radiosensitivity are very large. (2) At each dose point, "(dicentric chromosome + centric rings)/cell" is proportional to "dose rate", that is, Y = kx + b. (3) "(dicentric chromosome + centric rings)/Cell" is a quadratic linear relationship with dose rate, that is, y = ax 2 + bx + c.(4) We created a "Unity Standard Curve of Biological Dose Estimation". Creating a Unity Standard Curve of Biological Dose, under these circumstances, we can form a joint and rapid response to a nuclear and radiological accident.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Liu

2022

The FASEB Journal

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“…The added advantage of the possibility of controlled collection of the blood sample after the procedure can further enhance the application of γH2AX and phospho-53BP1 foci assays. These assays have extensively been studied for dosimetry of planned exposures in clinical and non-clinical applications and in human and non-human samples (22)(23)(24)(25)(26). Unlike cytogenetic assays, γH2AX and phospho-53BP1 foci assays have not been much explored for biodosimetry of radiological incidences (unplanned exposures) involving human exposures.…”

Section: Introductionmentioning

confidence: 99%

Establishment of in vitro Calibration Curve for 60Co-γ-rays Induced Phospho-53BP1 Foci, Rapid Biodosimetry and Initial Triage, and Comparative Evaluations With γH2AX and Cytogenetic Assays

Chaurasia

Shirsath

Desai

et al. 2022

Front. Public Health

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A rapid and reliable method for biodosimetry of populations exposed to ionizing radiation in the event of an incident or accident is crucial for initial triage and medical attention. DNA-double strand breaks (DSBs) are indicative of radiation exposure, and DSB-repair proteins (53BP1, γH2AX, ATM, etc.) are considered sensitive markers of DSB quantification. Phospho-53BP1 and γH2AX immunofluorescence technique serves as a sensitive, reliable, and reproducible tool for the detection and quantification of DSB-repair proteins, which can be used for biological dose estimations. In this study, dose-response curves were generated for 60Co-γ-rays induced phospho-53 Binding Protein 1 (phospho-53BP1) foci at 1, 2, 4, 8, 16, and 24 h, post-irradiation for a dose range of 0.05–4 Gy using fluorescence microscopy. Following ISO recommendations, minimum detection limits (MDLs) were estimated to be 16, 18, 25, 40, 50, and 75 mGy for dose-response curves generated at 1, 2, 4, 8, 16, and 24 h post-irradiation. Colocalization and correlation of phospho-53BP1 and γH2AX were also measured in irradiated peripheral blood lymphocytes (PBLs) to gain dual confirmation. Comparative evaluation of the established curve was made by γH2AX-immunofluorescence, dicentric chromosome assay (DCA), and reciprocal translocation (RT) assays by reconstructing the dose of 6 dose-blinded samples. Coefficients of respective in-house established dose-response curves were employed to reconstruct the blind doses. Estimated doses were within the variation of 4.124%. For lower doses (0.052 Gy), phospho-53BP1 and γH2AX assays gave closer estimates with the variation of −4.1 to + 9% in comparison to cytogenetic assays, where variations were −8.5 to 24%. For higher doses (3 and 4 Gy), both the cytogenetic and immunofluorescence (phospho-53BP1 and γH2AX), assays gave comparable close estimates, with −11.3 to + 14.3% and −10.3 to −13.7%, variations, respectively.

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Low-dose radiation from CT examination induces DNA double-strand breaks and detectable changes of DNA methylation in peripheral blood cells

Wang,

Li,

et al. 2023

International Journal of Radiation Biology

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Clinical Applications of Biological Dosimetry in Patients Exposed to Low Dose Radiation Due to Radiological, Imaging or Nuclear Medicine Procedures

Cited by 4 publications

References 219 publications

Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Revision of cytogenetic dosimetry in the IAEA manual 2011 based on data about radio‐sensitivity and dose‐rate findings contributing

Establishment of in vitro Calibration Curve for 60Co-γ-rays Induced Phospho-53BP1 Foci, Rapid Biodosimetry and Initial Triage, and Comparative Evaluations With γH2AX and Cytogenetic Assays

Low-dose radiation from CT examination induces DNA double-strand breaks and detectable changes of DNA methylation in peripheral blood cells

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