Clinical application of the serum 1,5‐anhydroglucitol assay method using glucose 3‐dehydrogenase

Hamafuji, Tetsuro; Tsugawa, Wakako; Sode, Koji

doi:10.1002/jcla.10054

Cited by 8 publications

(1 citation statement)

References 11 publications

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“…Other diabetes markers, such as sugar alcohols, 1,5-anhydro-d-glucitol (1,5-AG) (Jeppson et al, 2002), sorbitol (Malone at al., 1980), myo-inositol (Tetsuo et al, 1999) and mannitol (Pikanen, 1996) have also been reported. In particular, 1,5-AG reflects the very early stage (pre-diabetes) of the disease (Jeppson et al, 2002;Chusney et al, 1995;Tanabe et al, 1997;Hamefuji et al, 2002). The composition of these sugar alcohols indicates the characteristic pattern of clinical conditions in diabetic patients (Jeppson et al, 2002;Malone et al, 1980;Tetsuo et al, 1999;Pikanen, 1996;Niwa et al, 1994;Hamefuji et al, 1997).…”

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confidence: 95%

Simultaneous determination of glucose, 1,5-anhydrod-glucitol and related sugar alcohols in serum by high-performance liquid chromatography with benzoic acid derivatization

Katayama

Matsuda

Kobayashi

et al. 2006

Biomed. Chromatogr.

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A new, simple and sensitive pre-column high-performance chromatographic method for the determination of diabetes marker d-glucose, 1,5-anhydro-d-glucitol and related compounds is reported. Sugars (d-glucose, d-galactose, d-mannose, sucrose and arabinose) were derivatized with benzoic acid (BA) at 80 degrees C for 60 min. l-Fucose, fructose, d-lactose, l-rhamnose, arabinose and ascorbic acid were not reacted. Sugar alcohols (xylitol, erythritol, mannitol, sorbitol myo-inositol) were also derivatized with BA at 80 degrees C for 60 min. The fluorescence derivatives were separated on a TSK amide 80 column (4.6 mm i.d. x 250 mm, 5 microm) with acetonitrile-50 mm acetate buffer (pH 5.6; 4:96, v/v) as the mobile phase. The detection wavelength of beizoic acid derivatives was lambda(ex) 275 nm and lambda(em) 315 nm. The detection limits of sugars were 10-80 microg/mL. The calibration graphs were linear up to 10 mg/mL. The relative standard deviations of 500 microg/mL sugars were 7.0-7.3%. The proposed method was compared with the enzymatic photometric glucose analysis method (Glucose B-Test II Wako). The correlation coefficient was 0.83 (n = 20) and y = 0.82x + 5.91, where y and x are concentrations in microg/mL obtained by the proposed pre-column HPLC and enzyme-photometric method, respectively. The detection limits of sugar alcohols were 100-1000 ng/mL. The calibration graphs were linear to 50 microg/mL and relative standard deviations of 10 microg/mL were 7.2-8.2%. The 1,5-AG data by the proposed method was also compared with the enzymatic photometric 1,5-AG analysis method (Rana AG 1,5-AG determination kit, Nihon Kayaku) and good correlation (r = 0.91, n = 20) was also obtained. The proposed method was applied to the simultaneous determination of d-glucose, 1,5-AG and related sugar alcohols in serum from healthy males.

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Section: Introductionmentioning

confidence: 95%

Simultaneous determination of glucose, 1,5-anhydrod-glucitol and related sugar alcohols in serum by high-performance liquid chromatography with benzoic acid derivatization

Katayama

Matsuda

Kobayashi

et al. 2006

Biomed. Chromatogr.

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Purification and characterization of the glucoside 3-dehydrogenase produced by a newly isolated Stenotrophomonas maltrophilia CCTCC M 204024

Zhang

Zheng

Xue

et al. 2005

Appl Microbiol Biotechnol

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A soluble glucoside 3-dehydrogenase (G3DH) from Stenotrophomonas maltrophilia CCTCC M 204024, recently isolated from wheat soil in our laboratory, was purified to 37.4-fold with a yield of 24.7% and was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular mass of 66 kDa. 2,6-Dichlorophenolindophenol (DCPIP) and ferricyanide were able to act as artificial electron acceptors for the enzyme. The optimal pH of G3DH was in the range of 6.0-7.0 in the presence of DCPIP. The enzyme was stable in the pH range of 4.4-10.6 and was sensitive to heat. G3DH exhibited extremely broad substrate specificity by converting many sugars to their corresponding 3-ketoglucosides. They produced a characteristic spectrum by alkaline treatment with a peak at 340 nm. The apparent Km values for validoxylamine A and D: -glucose were 8.3 and 1.1 mM, respectively. Cu2+, Ag2+, and Hg2Cl2 inhibited the activity of G3DH.

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Screening of a Glucoside 3-Dehydrogenase-Producing Strain, Sphingobacterium faecium, Based on a High-Throughput Screening Method and Optimization of the Culture Conditions for Enzyme Production

Zhang

Chen

Wei

et al. 2014

Appl Biochem Biotechnol

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The objective of this study was to screen glucoside 3-dehydrogenase (G3DH)-producing strain based on a high-throughput G3DH screening method. Optimization of culture conditions of the isolated strain was also applied in this study. This screening method employed electron transfer reaction in 96-well microtiter plates, α-methyl-D-glucoside, galactose, 2-deoxy-D-glucose, and 3-O-methyl-D-glucose were used as substrates. Using this screening method, one out of 78 strains isolated from different soil samples was obtained with high G3DH activity. The accuracy of the screening method was proved by alkaline treatment analysis of 3-keto sugars. The isolated strain was identified as Sphingobacterium faecium ZJF-D6 by phenotypic characterization and 16S rDNA sequence analysis. The culture conditions of S. faecium for G3DH production were optimized. Sucrose was found as the most suitable carbon source for the G3DH production. The highest G3DH production and cell growth were achieved using the medium at the initial pH of 7.0 at 25 °C for 36 h with activity of 8.03 × 10(-2) U/mL culture. This strain appears promising for potential application in the industry to produce 3-keto sugars. To our knowledge, this is the first report on S. faecium for G3DH production. The method described herein represents a useful tool for the high-throughput isolation of G3DH.

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Clinical application of the serum 1,5‐anhydroglucitol assay method using glucose 3‐dehydrogenase

Cited by 8 publications

References 11 publications

Simultaneous determination of glucose, 1,5-anhydrod-glucitol and related sugar alcohols in serum by high-performance liquid chromatography with benzoic acid derivatization

Simultaneous determination of glucose, 1,5-anhydrod-glucitol and related sugar alcohols in serum by high-performance liquid chromatography with benzoic acid derivatization

Purification and characterization of the glucoside 3-dehydrogenase produced by a newly isolated Stenotrophomonas maltrophilia CCTCC M 204024

Screening of a Glucoside 3-Dehydrogenase-Producing Strain, Sphingobacterium faecium, Based on a High-Throughput Screening Method and Optimization of the Culture Conditions for Enzyme Production

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