Clinical Application of Hyperdry Amniotic Membrane in Cleft Palate Repair

Abstract

Objective To examine the safety and efficacy of hyperdry amniotic membrane (HDAM) for wound closure after palatoplasty in cleft palate patients. Methods HDAMs were prepared by washing and drying under infrared rays and microwaves at temperatures less than 60°C using a hyperdrying device. A total of 16 cleft palate patients (8 males, 8 females), aged 1 to 3 years (mean age 1 year 9 months), received one-stage pushback palatoplasty. The remaining raw wound after surgery was covered by an HDAM and a plastic cover plate. The cover plate was removed 1 week after surgery and parameters including temperature, feeding, allergic reactions, postoperative bleeding, re-epithelialization, wound dehiscence, and infection were monitored during the follow-up period of 31.2 months. Results All patients could adequately ingest at 5 days postoperation and after removal of the cover plate. None of the patients had a persistent fever or allergic reactions. Ingestion was feasible immediately in all patients, and no postoperative bleeding was observed during ingestion. No secondary hemorrhages were observed during follow-up. No postoperative wound dehiscence on the midline of the palate was observed. No infections were observed after the removal of the cover plate. No patients suffered from severe scar formation or contracture of the wound in the follow-up period. Hemorrhage, undue epithelialization, and scar contracture did not occur in any patient. The mean evaluation score was 7.75 points. Conclusion HDAM can be used safely and effectively for wound closure following palatoplasty in cleft palate infants. Future studies testing the safety of patient's own amnion for palatoplasty, are required.

Lyophilised Amniotic Membrane in Intraoral Surgical Defects: A Prospective Clinical Study

“…Our findings regarding its effectiveness in oral wound healing are in accordance with the findings of similar studies conducted on hyperdry and cryopreserved AM. [ 2 22 39 ] Although we admit the limitations in this study such as small sample size and lack of control group, the results of this study could serve as an initial report for future studies on the use of lyophilised AM.…”

Biological properties and surgical applications of the human amniotic membrane

“…It has analgesic functions, and favors early closure and healing of chronic wounds secondary to burns in different body regions in both children and adults ( Mostaque and Rahman, 2011 ; Mohammadi et al, 2013a ; Mohammadi et al, 2013b ). In surgeries of cleft palate repair (palatoplasties), cryopreserved AM was used to achieve adequate oral intake 5 days after surgery, without bleeding during surgery, without fever, allergic reactions, wound dehiscence, infections, and adequate epithelialization of wounds ( Martelloni et al, 2019 ; Fujiwara et al, 2022 ). AM has been used as a preventive treatment for tendon adhesion after repair.…”

potential of amniotic membrane in surgery - current applications

“…Po zabiegach nie obserwowano reakcji alergicznych, stanów zapalnych, krwotoków ani infekcji. W dłuższej obserwacji nie występowały nadmierne zabliźnienia lub przykurcze ran [22]. AM stosowane są także w chirurgicznym leczeniu przepuklin oponowordzeniowych z dobrym rezultatem [23].…”