Clinical application of calcium hydroxide in dental pathology and endodontics

Lačević, Amela; Vranić, Edina; Zulić, Irfan

doi:10.17305/bjbms.2003.3488

Cited by 13 publications

(9 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Presently, Ca(OH) 2 is the most commonly used medication for direct pulp capping treatment (Lacević et al, 2003). The weak expression of IL-6 on inflamed rat dental pulp 6 h after Ca(OH) 2 application and less marked on day 7 caused of its alkalinity.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from south Sulawesi, Indonesia

Sabir

Sumidarti

2017

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Propolis is a natural product of plant resins collected by honeybees from various plant sources. It is used as a remedy in folk medicine since ancient times because of its several biological and pharmacological properties. Recently, propolis has been used by dentist to treat various oral diseases. It was always mentioned as an anti-inflammatory agent. Cytokines are proteins that provide communication between cells and play a critical role in a wide variety of processes. It released from cells in an inflammatory process that active, mediate or potential actions of other cells or tissues. When dental pulp has inflammation, several pro-inflammatory cytokines including Interleukin-6 (IL-6) was released by innate immune cells. To analyse the expression of IL-6 on inflamed rat dental pulp tissue following application of propolis. sp. propolis was obtained from Luwu Regency, south Sulawesi Province, Indonesia. Flavonoid and non-flavonoid extracts were purified from propolis using thin layer chromatography. The study was applied on 80 male rats, 10-12 weeks of age, divided randomly and equally into 5 groups. Group I, as negative control group was not conducted any treatment. At group II, III, IV and V. A Class I cavity (Black Classification) were made on the occlusal surface of right maxillary first molar. The dental pulp was perforated using dental explorer and allowed in the oral environment for 1 h, after that, Ethanolic Extract Propolis (EEP) (Group II), Extract Flavonoid-Propolis (EFP) (Group III), Extract Non-Flavonoid Propolis (ENFP) (Group IV), or Calcium Hydroxide (Ca(OH)) (Group V) were applied on dental pulp. All cavities were then filled with Glass Ionomer Cement as permanent filling. The rats being sacrificed in 6 h, 2 days, 4 days and 7 days. Sample biopsy were obtained, IL-6 expression was detected by using immunohistochemistry method. Data was analyzed statistically using Freidman and Kruskal Wallis tests with significance level of < 0.05. All agent showed IL-6 expression in inflamed rat dental pulp tissue, and this expression was decreased with the longer of observation time periods. EEP more stronger to decreased IL-6 expression on inflamed rat dental pulp tissue than other agent. There is significant difference ( < 0.05) of IL-6 expression between group I and other groups in 6 h and 2 days but not in 4 and 7 days time periods. sp. propolis from south Sulawesi, Indonesia could suppressed the expression of IL-6 on inflamed rat dental pulp tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from south Sulawesi, Indonesia

Sabir

Sumidarti

2017

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Calcium hydroxide, the most commonly used intracanal medicament with antibacterial properties that is based on hydroxide ion release [37], showed better results after 7–14 days of intracanal dressing compared to a single endodontic treatment appointment without dressing of the root canal [38, 39]. At least two weeks are needed to create bactericidal activity by calcium hydroxide in the periapical area [40], yet calcium hydroxide-based sealers are effective against most of the microorganisms in the root canals [41], with little or no effect on E . faecalis [8, 42].…”

Section: Discussionmentioning

confidence: 99%

Phages in a thermoreversible sustained-release formulation targeting E. faecalis in vitro and in vivo

et al. 2019

View full text Add to dashboard Cite

Introduction Enterococcus faecalis is a key pathogen recovered from root canals when conventional treatment fails. Phage therapy has generated new interest in combating pathogens. A sustained-release formulation using specific phages against E . faecalis may offer an alternative approach. Objectives To evaluate the efficacy of anti- E . faecalis phages formulated in a thermo- sustained-release system against E . faecalis in vitro and in vivo . Methods EFDG1 and EFLK1 phages were formulated with poloxamer P407. Gelation time, phage survival, activity and toxicity were evaluated. Lytic activity was evaluated in vitro against E . faecalis at various growth phases, including anti-biofilm activity. Methods included viable bacterial count (CFU/mL), biofilm biomass determination and electron microscopy (live/dead staining). Further evaluation included infected incisors in an in vivo rat model. Anti -E . faecalis phage-cocktail suspension and sustained-release phage formulation were evaluated by viable bacterial count (CFU/mL), histology, scanning electron microscopy (SEM) and 16S genome sequencing of the microbiota of the root canal. Results Gelation time for clinical use was established. Low toxicity and a high phage survival rate were recorded. Sustained-release phages reduced E . faecalis in logarithmic (4 logs), stationary (3 logs) and biofilm (4 logs) growth phases. Prolonged anti-biofilm activity of 88% and 95% reduction in biomass and viable counts, respectively, was recorded. Reduction of intracanal viable bacterial counts was observed (99% of enterococci) also seen in SEM. Phage treatment increased Proteobacteria and decreased Firmicutes . Histology showed reduced periapical inflammation and improved healing following phage treatment. Conclusion Poloxamer P407 formulated with phages has an effective and long-lasting effect in vitro and in vivo targeting E . faecalis .

show abstract

“…Calcium hydroxide owing to its maintenance of alkaline pH in the canal is the preferred intracanal medicament to maintain antibacterial environment and aids in resolution of acute symptoms. 10 The diode laser is assertively absorbed by the pigments in the bacterial cell wall. 11 This inactivates the bacteria without decisively destroying them; nevertheless, it aids in the disinfection of the canal.…”

Section: Discussionmentioning

confidence: 99%

Diode Laser as an Adjuvant in Treating Endo-perio Lesions in Maxillary Incisors

Nandakumar¹,

Ganesan²,

Shalini³

2015

International Journal of Laser Dentistry

View full text Add to dashboard Cite

The application of laser in endodontic therapy has been extensively studied. In few cases both periodontal and pulpal disease present simultaneously and pose a significant challenge in diagnosis, treatment planning, as well as healing of the endoperio lesion. This study aims at orderly treating endo-perio lesions with the help of diode laser. The case reports deal with true endodontic and combined endo-perio lesion in maxillary incisors. True endodontic lesion in 11 was treated with root canal obturation after disinfection with diode laser. Combined endo-perio lesion in 21 was treated with root canal obturation assisted by diode disinfection, scaling, and laser curettage and cleaning of periodontal pocket. The procedure resulted in resolution of periodontal pocket depth after 2 months. One year review of both cases showed healing of periapical lesion and maintenance of periodontal health. Diode laser is an excellent tool in handling soft tissues, gingiva, and periodontium due to less tissue shrinkage, less intraoperative time, and reduced postoperative discomfort. Laser disinfection of root canals assists in healing and prognosis in complicated situations. The use of lasers may lead to developing better and predictable treatment protocol for endodontic periodontal lesions.

show abstract

Clinical application of calcium hydroxide in dental pathology and endodontics

Cited by 13 publications

References 5 publications

Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from south Sulawesi, Indonesia

Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from south Sulawesi, Indonesia

Phages in a thermoreversible sustained-release formulation targeting E. faecalis in vitro and in vivo

Diode Laser as an Adjuvant in Treating Endo-perio Lesions in Maxillary Incisors

Contact Info

Product

Resources

About