Clinical Application of a Silk Fibroin Protein Biologic Scaffold for Abdominal Wall Fascial Reinforcement

Clemens, Mark W.; Downey, Susan E.; Agullo, Frank; Lehfeldt, Max R.; Kind, Gabriel M.; Palladino, Humberto; Marshall, Deirdre; Jewell, Mark L.; Mathur, Anshu B.; Bengtson, Bradley P.

doi:10.1097/gox.0000000000000217

Cited by 37 publications

(17 citation statements)

References 48 publications

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“…Lastly, silk constructs have stability of shape and lack of swelling under physiologic conditions due to its hydrophobic nature – a common problem with many other degradable biomaterials. These characteristics of silk have led to investigation of their utility in the bladder and urethra (Table 1), in addition to a number of non-genitourinary related medical specialties including gastroenterology [65–67], ophthalmology [68], orthopedic surgery [69–71], vascular surgery [72], neurology [73,74], plastic surgery [75], and otolaryngology [76]. …”

Section: Novel Properties Of Silk-based Biomaterialsmentioning

confidence: 99%

Silk Fibroin Scaffolds for Urologic Tissue Engineering

2016

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Urologic tissue engineering efforts have been largely focused on bladder and urethral defect repair. The current surgical gold standard for treatment of poorly compliant pathological bladders and severe urethral stricture disease is enterocystoplasty and onlay urethroplasty with autologous tissue, respectively. The complications associated with autologous tissue use and harvesting have led to efforts to develop tissue-engineered alternatives. Natural and synthetic materials have been used with varying degrees of success, but none has proved consistently reliable for urologic tissue defect repair in humans. Silk fibroin (SF) scaffolds have been tested in bladder and urethral repair because of their favorable biomechanical properties including structural strength, elasticity, biodegradability and biocompatibility. SF scaffolds have been used in multiple animal models, and have demonstrated robust regeneration of smooth muscle and urothelium. The pre-clinical data involving SF scaffolds in urologic defect repair are encouraging and suggest that they hold potential for future clinical use.

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Section: Novel Properties Of Silk-based Biomaterialsmentioning

confidence: 99%

Silk Fibroin Scaffolds for Urologic Tissue Engineering

2016

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“…Researchers have also fabricated mesh prostheses using silk proteins isolated from both worms and spiders. Silk derived from worms has been shown to produce various immune responses and is considered to be less biocompatible than spider silk due to a lack of natural lubricant coatings [88, 89]. …”

Section: Introductionmentioning

confidence: 99%

Design strategies and applications of biomaterials and devices for Hernia repair

Kalaba

Gerhard

Winder

et al. 2016

Bioactive Materials

113

128

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Hernia repair is one of the most commonly performed surgical procedures worldwide, with a multi-billion dollar global market. Implant design remains a critical challenge for the successful repair and prevention of recurrent hernias, and despite significant progress, there is no ideal mesh for every surgery. This review summarizes the evolution of prostheses design toward successful hernia repair beginning with a description of the anatomy of the disease and the classifications of hernias. Next, the major milestones in implant design are discussed. Commonly encountered complications and strategies to minimize these adverse effects are described, followed by a thorough description of the implant characteristics necessary for successful repair. Finally, available implants are categorized and their advantages and limitations are elucidated, including non-absorbable and absorbable (synthetic and biologically derived) prostheses, composite prostheses, and coated prostheses. This review not only summarizes the state of the art in hernia repair, but also suggests future research directions toward improved hernia repair utilizing novel materials and fabrication methods.

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“…Due to its excellent biocompatibility and mechanical toughness silk has been used and studied as a protein polymer for the production of various medical devices. To date, commercially available medical products consisting of silk include sutures and a mesh developed by Allergan, and both types of products are approved by the U.S. FDA and are in clinical use …”

Section: Introductionmentioning

confidence: 99%

Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements

Teuschl

Zipperle

Huber-Gries

et al. 2016

J Biomedical Materials Res

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The control of bleeding is one of the most important interventions after a traumatic injury. Hemostatic devices delivering blood clotting accelerating agents such as fibrinogen are increasingly used due to their efficacy and their ease of application. In the present study, we describe a method to incorporate the coagulant supplements fibrinogen and thrombin in silk protein sponges by mixing the coagulants with an aqueous silk solution, followed by molding, freeze-drying, and water annealing. In this combination system, we demonstrate the delivery of fibrinogen while maintaining its hemostatic potential. Concentration ratios of silk to fibrinogen of 1.0%/2.8%, 2.3%/1.5%, and 3.0%/0.8% were used. The thrombin-induced fibrin polymeric network filled the space in and next to the silk spongy structure but also remained interconnected to the silk, providing an intact network. The mechanical characterization of the fibrinogen-releasing silk sponges before and after the induction of the fibrinogen polymerization demonstrated that the fibrin network resulted in reduced permanent deformation from 21.1% to 6.5%, 19.6% to 5.7%, and 12.7% to 9.4% for the 2.8%, 1.5%, and 0.8% fibrinogen-containing silk sponges, respectively. Moreover, the fibrin formation lead to a more linear elastic behavior over longer strain ranges. In combination, the Calcein-AM/PI staining and MTT assay results indicate uniform cell adhesion on the surface and cytocompatibility of the silk/fibrin sponges, respectively. Moreover, the co-delivery of thrombin with fibrinogen via silk as carrier material is described, offering a more mechanically robust and durable system while preserving hemostatic features of the coagulant substances for the generation of hemostatic devices. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 687-696, 2017.

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Clinical Application of a Silk Fibroin Protein Biologic Scaffold for Abdominal Wall Fascial Reinforcement

Cited by 37 publications

References 48 publications

Silk Fibroin Scaffolds for Urologic Tissue Engineering

Silk Fibroin Scaffolds for Urologic Tissue Engineering

Design strategies and applications of biomaterials and devices for Hernia repair

Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements

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