Clinical application of a new assisted hatching method using a piezo-micromanipulator for morphologically low-quality embryos in poor-prognosis infertile patients

Nakayama, Takahiro; Fujiwara, Hiroshi; Yamada, Shigetoshi; Tastumi, Keiji; Honda, Testuro; Fujii, Shingo

doi:10.1016/s0015-0282(99)00131-4

Cited by 68 publications

(35 citation statements)

References 26 publications

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“…Since the first assisted hatching technique that used a mechanical method, several approaches have been proposed [45], including mechanical incision of the zona [43]; chemical zona drilling with acidic medium [46]; chemical zona thinning [47]; laser-assisted hatching [33] and, more recently, piezo technology [14]. The mechanical and the chemical methods require extensive technical skill to produce uniform, well controlled and standardised micro holes using micropipettes mounted on micromanipulators.…”

Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

“…Recently, piezo technology has been introduced for zona pellucida drilling [14]. While a holding pipette holds the embryo, vibratory movements produced by a piezo-electric pulse regulated by a controller are used to carve a limited conical area in the zona pellucida.…”

Section: Piezo Technologymentioning

confidence: 99%

“…This pioneering work was followed by numerous publications. Some of these publications reported a significant increase in the pregnancy and/or implantation rate in all patients [11]; other publications reported significant improvement in patients with a poor prognosis, namely women over 38 years of age, patients with thick zonae, and patients with repeated implantation failures [12][13][14]; while a third group of publications reported a non significant improvement in the pregnancy rate in patients with poor prognosis [11,[15][16][17][18]. On the contrary, some publications reported no improvement in pregnancy or implantation [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

105

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The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

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Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

Section: Piezo Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

105

View full text Add to dashboard Cite

show abstract

“…chemical zona drilling with acidic medium 20 ; chemical zona thinning 33 ; laser-assisted hatching 34 and, more recently, piezo technology 35 . The mechanical and the chemical methods require extensive technical skill to produce uniform, well controlled and standardised micro holes using micropipettes mounted on micromanipulators.…”

Section: Techniques Of Assisted Hatchingmentioning

confidence: 99%

“…More recently, piezo technology has been introduced for zona pellucida drilling 35 . While a holding pipette holds the embryo, vibratory movements produced by a piezo-electric pulse regulated by a controller are used to carve a limited conical area in the zona pellucida.…”

Section: Piezo Technologymentioning

confidence: 99%