Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Wang, Shiwen; Feng, Yang; Liu, Dong; Qin, Juanxiu; Hou, Weiwei; Lian, Jiqin; Kong, Mimi; Wu, Yong; Zhang, Yuchen; Zhao, Fuju; Fang, Yi; Miao, Yingxin; Xu, Linfeng; Chen, Jie; Bao, Zhijun; Olszewski, Michal A.; Hu, Zhao; Zhang, Yanmei

doi:10.1186/s13099-018-0264-7

Cited by 7 publications

(4 citation statements)

References 47 publications

(49 reference statements)

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“…[13] With the rapid development of molecular techniques, multiplex polymerase chain reaction (PCR) has been increasingly used for the diagnosis of infectious diseases in recent years, due to its high efficiency. [14,15] The advanced fragment analysis (AFA) method perfectly combines multiple PCR amplification and capillary electrophoresis, allowing it possible to detect dozens of pathogenic genes simultaneously in a single analysis. [16] In view of these advantages, AFA was utilized in this study to simultaneously detect 9 common respiratory viruses from nasopharyngeal swabs of children.…”

Section: Introductionmentioning

confidence: 99%

Precautions against COVID-19 reduce respiratory virus infections among children in Southwest China

et al. 2022

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Acute respiratory tract infections pose a serious threat to the health of children worldwide, with viral infections representing a major etiology of this type of disease. Protective measures such as mask-wearing, social distancing, and hand hygiene can be effective in curbing the spread of severe acute respiratory syndrome coronavirus 2. These precautions may also have an impact on the spread of other respiratory viruses. In this study, we retrospectively compared the respiratory virus infections of children in Southwest China before and after the outbreak of COVID-19. Nasopharyngeal swabs were collected from 1578 patients under 14 years old with acute respiratory tract infection symptoms before and after COVID-19 pandemic. Nine common respiratory viruses including human bocavirus, human rhinoviruses, human coronaviruses, human adenoviruses, human metapneumovirus, respiratory syncytial virus, influenza A virus, influenza B virus, and parainfluenza virus were measured by advanced fragment analysis. The respiratory virus infection rates among children of all ages and genders in Southwest China under the precautions against COVID-19 pandemic were significantly lower than that of the same period before the pandemic. Our findings indicate that public health measures implemented during the COVID-19 pandemic, including strict mask-wearing, social distancing, and hand hygiene, may be effective in preventing the transmission of other respiratory viruses in children, thereby controlling the spread of infections.

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Section: Introductionmentioning

confidence: 99%

Precautions against COVID-19 reduce respiratory virus infections among children in Southwest China

et al. 2022

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“…Multiplex PCR‐based NAATs have been increasingly used for syndromic diagnosis, due to their high throughput, high sensitivity, high specificity, cost‐effectiveness, and great clinical significance . The ResP assay is based on multiplex PCR amplification and capillary electrophoretic separation of PCR amplicons by length.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients

Zhang

Cao

Xu³

et al. 2019

Clinical Laboratory Analysis

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Background: Respiratory viruses, such as influenza viruses, initially infect the upper airways but can manifest as severe lower respiratory tract infections in high-risk patients with significant morbidity and mortality. For syndromic diagnosis, several multiplex nucleic acid amplification tests have been developed for clinics, of which SureX 13 Respiratory Pathogen Multiplex Kit (ResP) can simultaneously detect 13 pathogens directly from airway secretion specimens. The organisms identified are influenza virus A, influenza virus A pdmH1N1 (2009), influenza virus A H3N2, influenza virus B, adenovirus, boca virus, rhinovirus, parainfluenza virus, coronavirus, respiratory syncytial virus, human metapneumovirus, Mycoplasma pneumoniae, and Chlamydia.Methods: This study provides performance evaluation data of this assay by comparing with pathogen-specific PCRs from oropharyngeal swab samples. Results: Ten pathogens were detected in this assay, of which rhinovirus, adenovirus, and influenza virus A pdmH1N1 (2009) were the most common. The overall agreement between the ResP and the comparator tests was 93.8%. The ResP demonstrated 86.5% agreement for positive results and 97.8% agreement for negative results. Conclusion: The ResP assay demonstrated a highly concordant performance comparing with pathogen-specific PCRs for detection of respiratory pathogens in oropharyngeal swabs from outpatients and could aid in the diagnosis of respiratory infections in a variety of clinical scenarios. K E Y W O R D S acute respiratory infections, influenza virus A, multiplex PCR, oropharyngeal swab, respiratory virus How to cite this article: Zhang Y, Cao L, Xu Z, et al. Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients. J Clin Lab

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“…Molecular methods based on polymerase chain reaction (PCR) technology have been applied for the direct and rapid detection of causative agents in infectious diseases [11][12][13]. The development of multiplex genetic detection systems has enabled the simultaneous detection of different pathogens in a single panel, which have been successfully applied for diagnosing infectious diseases including diarrhoea, Helicobacter pylori infection, and respiratory infections [14,15]. Consequently, there is increasing interest in using molecular methods directly for analysing whole blood, which could break through the bottleneck of culture and shorten the turnaround time.…”

Section: Introductionmentioning

confidence: 99%

Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system

et al. 2022

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Background Bloodstream infection (BSI) is a life-threatening condition with high morbidity and mortality rates worldwide. Early diagnosis of BSI is critical to avoid the unnecessary application of antimicrobial agents and for proper treatment. However, the current standard methods based on blood culture are time-consuming, thus failing to provide a timely etiological diagnosis of BSI, and common PCR-based detection might be inhibited by matrix components. Methods The current study explored an integrated pre-analytical treatment protocol for whole blood samples, wherein pathogens are enriched and purified by incubation and concentration, and inhibitors are inactivated and removed. Further, this study developed and evaluated a novel high-throughput multiplex genetic detection system (HMGS) to detect 24 of the most clinically prevalent BSI pathogens in blood culture samples and pre-treated whole blood samples. The specificity and sensitivity were evaluated using related reference strains and quantified bacterial/fungal suspensions. The clinical utility of BSI-HMGS combined with the pre-analytical treatment protocol was verified using blood cultures and whole blood samples. Results The combined pre-treatment protocol and BSI-HMGS was highly specific for target pathogens and possessed a low detection limit for clinical whole blood samples. The pre-treatment protocol could deplete the PCR inhibitors effectively. For blood culture samples, the current method showed 100.0% negative percent agreements and > 87.5% positive percent agreements compared to the reference results based on blood culture findings. For whole blood samples, the current method showed 100.0% negative percent agreements and > 80.0% positive percent agreements compared to the reference results for most pathogens. The turnaround time was ≤ 8 h, and all the procedures could be conducted in a general clinical laboratory. Conclusion The BSI-HMGS combined with the pre-treatment protocol was a practical and promising method for early and precise detection of BSIs, especially for areas without access to advanced medical facilities.

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Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Cited by 7 publications

References 47 publications

Precautions against COVID-19 reduce respiratory virus infections among children in Southwest China

Precautions against COVID-19 reduce respiratory virus infections among children in Southwest China

Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients

Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system

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