Clinical Anesthesia and Analgesia in Invertebrates

Lewbart, Gregory A.; Mosley, Conny

doi:10.1053/j.jepm.2011.11.007

Cited by 57 publications

(37 citation statements)

References 39 publications

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“…The results of other studies are basically similar to the findings here: best results with ethanol (rapid induction of full anaesthesia, adequate duration, swift recovery, and no adverse reactions); toxic effects with other substances, such as with phenoxetol at certain concentrations (as reported also by Sen and Tanrikul, 2009). The success or otherwise of some of these anaesthetics used in earlier studies was mentioned in broad terms some time ago in a conference (Gleadall, 1991), and caution when using ethanol at lower temperatures was mentioned in personal communications from the present author quoted in Lewbart (2007, 2008) and in Lewbart and Mosley (2012).…”

Section: Unwanted Responses To Anaesthetics: Tarp -Typical Adverse Rementioning

confidence: 92%

The effects of prospective anaesthetic substances on cephalopods: Summary of original data and a brief review of studies over the last two decades

Gleadall

2013

Journal of Experimental Marine Biology and Ecology

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Section: Unwanted Responses To Anaesthetics: Tarp -Typical Adverse Rementioning

confidence: 92%

The effects of prospective anaesthetic substances on cephalopods: Summary of original data and a brief review of studies over the last two decades

Gleadall

2013

Journal of Experimental Marine Biology and Ecology

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“…Two animals were sampled after 60, 120, 240, 360, and 480 min following the injection of the tracer. Animals were killed by performing anesthesia in 5% ethanol in seawater and then bisecting the brain downward and forward, followed by two lateral cuts to sever the brain from the optical lobes (22). A sample of mantle (on the ventral side), digestive gland, and gill was collected and quickly frozen on dry ice before further processing.…”

Section: Animalsmentioning

confidence: 99%

Metabolic rate and rates of protein turnover in food-deprived cuttlefish,Sepia officinalis(Linnaeus 1758)

Lamarre

MacCormack

Sykes

et al. 2016

American Journal of Physiology-Regulatory, Integrative and Comp

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To determine the metabolic response to food deprivation, cuttlefish (Sepia officinalis) juveniles were either fed, fasted (3 to 5 days food deprivation), or starved (12 days food deprivation). Fasting resulted in a decrease in triglyceride levels in the digestive gland, and after 12 days, these lipid reserves were essentially depleted. Oxygen consumption was decreased to 53% and NH4 excretion to 36% of the fed group following 3-5 days of food deprivation. Oxygen consumption remained low in the starved group, but NH4 excretion returned to the level recorded for fed animals during starvation. The fractional rate of protein synthesis of fasting animals decreased to 25% in both mantle and gill compared with fed animals and remained low in the mantle with the onset of starvation. In gill, however, protein synthesis rate increased to a level that was 45% of the fed group during starvation. In mantle, starvation led to an increase in cathepsin A-, B-, H-, and L-like enzyme activity and a 2.3-fold increase in polyubiquitin mRNA that suggested an increase in ubiquitin-proteasome activity. In gill, there was a transient increase in the polyubiquitin transcript levels in the transition from fed through fasted to the starved state and cathepsin A-, B-, H-, and L-like activity was lower in starved compared with fed animals. The response in gill appears more complex, as they better maintain rates of protein synthesis and show no evidence of enhanced protein breakdown through recognized catabolic processes.

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“…Among authors, there is considerable variation between mollusc species in their reaction to particular relaxants and varying concentrations (for review, see LEWBART and MOSLEY, 2012). In the present study, 87% oysters C. gasar were anaesthetized after 12 h of exposure to 50 g L -1 MgCl2 and 100% C. gigas and C. rhizophorae after 6 h of induction.…”

Section: Discussionmentioning

confidence: 92%

Anesthesia in oysters of the genus Crassostrea cultured in Brazil

Puchnick‐Legat¹,

Legat²,

Gomes³

et al. 2015

Bol. Inst. Pesca

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This study evaluated: 1) the time required for anaesthesia induction and recovery of oysters Crassostrea rhizophorae, Crassostrea gasar and Crassostrea gigas using magnesium chloride (MgCl2); 2) the survival after anaesthesia and gonad sampling; and 3) the D-larvae generation after anaesthesia. For each species, three groups of 10 animals were kept in 50 g L -1 MgCl2, salinity of 36 and temperature of 22 ºC. One control group was kept in solution without MgCl2. Every 1 h anaesthetised oysters were recorded, sampled to determine sex and placed in clean seawater to assess recovering every 30 min. Gonad samplings were made beside the posterior adductor muscle, using 1 mL syringes and needles (0.60 x 25 mm). Factorial crosses were generated within and between anaesthetised and non-anaesthetised oyster groups to produce D-larvae in C. gigas. The survival after 10 days of anaesthesia was 100% for the three studied species. For C. rhizophorae and C. gigas, 100% of the animals were anaesthetised after 360 min and were recovered after 240 and 150 min, respectively. For C. gasar, 87% were anesthetised after 720 min and recovered after >240 min. There were no significant differences in D-larvae numbers between factorial crosses. The salt MgCl2 served as an efficient relaxant and caused no deleterious effect on the survival of the three studied species, or on the D-larvae generation in C. gigas.Keywords: anaesthetics; magnesium chloride; Crassostrea gasar; Crassostrea rhizophorae; Crassostrea gigas; D-larvae ANESTESIA EM OSTRAS DO GÊNERO Crassostrea CULTIVADAS NO BRASIL RESUMOEste estudo avaliou: 1) o tempo necessário para indução à anestesia e recuperação das ostras Crassostrea rhizophorae, Crassostrea gasar e Crassostrea gigas utilizando cloreto de magnésio (MgCl2); 2) a sobrevivência após anestesia e amostragem gonadal; e 3) a geração de larvas-D após anestesia. Para cada espécie, três grupos de 10 animais foram mantidos em 50 g L -1 de MgCl2, salinidade de 36 e temperatura de 22 ºC. Um grupo controle foi mantido em solução sem MgCl2. A cada 1 h, ostras anestesiadas foram registradas, amostradas para determinar sexo e colocadas em água do mar limpa para avaliar a recuperação a cada 30 min. Amostragens das gônadas foram feitas ao lado do músculo adutor posterior, usando seringas de 1 mL e agulha de 0,60 x 25 mm. Cruzamentos fatoriais foram gerados dentro e entre grupos de ostras anestesiadas e não anestesiadas para produzir larvas-D em C. gigas. A sobrevivência após 10 dias de anestesia foi de 100% para as três espécies. Para C. rhizophorae e C. gigas, 100% dos animais foram anestesiados após 360 min e recuperados após 240 e 150 min, respectivamente. Para C. gasar, 87% foram anestesiados após 720 min e recuperados após >240 min. Não houve diferença significativa no número de larvas-D entre os cruzamentos. O sal MgCl2 serviu como relaxante eficiente e não causou efeitos deletérios sobre a sobrevivência das três espécies estudadas ou na geração de larvas-D de C. gigas.

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Clinical Anesthesia and Analgesia in Invertebrates

Cited by 57 publications

References 39 publications

The effects of prospective anaesthetic substances on cephalopods: Summary of original data and a brief review of studies over the last two decades

The effects of prospective anaesthetic substances on cephalopods: Summary of original data and a brief review of studies over the last two decades

Metabolic rate and rates of protein turnover in food-deprived cuttlefish,Sepia officinalis(Linnaeus 1758)

Anesthesia in oysters of the genus Crassostrea cultured in Brazil

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