Clinical and pathological insights into Johne′s disease in buffaloes

Dalto, André Gustavo Cabrera; Bandarra, Paulo Mota; Pavarini, Saulo Petinatti; Boabaid, Fabiana M.; Bitencourt, Ana Paula Gobbi de; Gomes, Marcos Pereira; Driemeier, David; Cruz, Cláudio Estêvão Farias da

doi:10.1007/s11250-012-0154-9

Cited by 20 publications

(36 citation statements)

References 17 publications

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“…Os sinais clínicos observados nos búfalos deste estudo foram semelhantes aos encontrados por Mota et al (2010), Dalto et al (2012) e Brito (2015 em búfalos com paratuberculose. A diarreia ocorre devido à má absorção de nutrientes, em função da enterite granulomatosa, o que justifica a perda progressiva de peso e o mau estado corporal dos animais afetados (Chiodini et al 1984).…”

Section: Discussionunclassified

“…Segundo esses autores podem ser ainda visualizados plasmócitos e, ocasionalmente, neutrófilos e eosinófilos. Dalto et al (2012) também realizaram análise da mucosa retal em um búfalo e verificaram lesões neste segmento, presença de BAAR e marcação positiva na IHQ. Esses achados foram semelhantes aos observados nos búfalos deste estudo, que tiveram lesões sugestivas de paratuberculose, presença de BAAR e PCR positivo, tanto nas amostras das biópsias retais como da análise de íleo e linfonodo mesentérico obtidos na necropsia.…”

Section: Discussionunclassified

“…Apenas um animal foi negativo na análise de biópsia retal e positivo nos tecidos das necropsias. Animais em estágios iniciais da enfermidade, geralmente apresentam lesões leves e localizadas principalmente nas porções de íleo, que com a evolução da enfermidade, se estende até o reto (Mota et al 2010, Dalto et al 2012. Exames histológicos e PCR em tecidos já foram realizados por Sivakumar et al (2005) e Mota et al (2010) em búfalos com paratuberculose e esses autores demonstraram que a pesquisa em amostras de tecido pode ser realizada e que apresenta resultados satisfatórios, assim como observado no presente trabalho.…”

Section: Discussionunclassified

“…Ainda não há um teste padrão para o diagnóstico da paratuberculose devido à natureza crônica da enfermidade e aos diferentes estágios da doença (OIE 2014). Na paratuberculose as lesões localizam-se principalmente no trato intestinal e linfonodos mesentéricos; no intestino grosso são observadas lesões da mesma natureza, porém menos extensas (Driemeier et al 1999, Mota et al 2010; também foram relatadas lesões na mucosa retal em búfalos (Dalto et al 2012). Dalto et al (2012) realizaram uma biópsia em um búfalo no Rio Grande do Sul e sugeriram que este exame poderia ser mais uma ferramenta para confirmação do diagnóstico de paratuberculose nesta espécie, visto que no fragmento analisado pelos auto res, havia BAAR e marcação positiva na Imunoistoquímica (IHQ).…”

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Diagnóstico de paratuberculose por biópsia retal em búfalos

et al. 2015

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Paratuberculosis in a herd of buffaloes was studied in the municipality of São Mateus, Maranhão, Brazil. Rectal biopsies were performed in 140 male and female Murrah, Mediterranean and crossbreed buffaloes older than 3 years. Postmortem examination of 11 buffaloes was performed to compare the rectal biopsies with possible lesions in mesenteric nodes and the intestine. The history of the herd and clinical examination revealed progressive weight loss and non-responsive antimicrobial diarrhea, dehydration and submandibular edema. Rectal biopsies showed in six buffaloes microscopically suggestive lesions for paratuberculosis through hematoxilin-eosin staining (HE), characterized by moderate multifocal granulomatous enteritis with epithelioid cell infiltration. In four buffaloes Langhans giant cells were found. In 15 buffaloes lymphocytic infiltrate was observed in the lamina propria of the large intestine. Ziehl-Neelsen staining (ZN) revealed in 4.3% (6/140) acid-fast bacilli in the rectal mucosa. Real time PCR amplified to 5.71% (7/140) Mycobacterium avium subsp. paratuberculosis (Map) DNA. 11 buffalos were submitted to postmortem examination, gross examination revealed augmented mesenteric nodes with whitish areas in the cut surface. The mucosa of the small intestine was irregular and thickened, with evident traverse folds and Peyer plates. The brownish intestinal content was fluid, the ileocecal valve area thickened and edematous with evident lymphatic vessels. Histological lesions in the mesenteric lymph node and small intestine four buffalo were compatible with those already described in the literature, and presented acid-fast bacilli by ZN staining and amplification of Map genetic material in qPCR. The concordance between the rectal biopsy and the postmortem samples was in agreement with the Kappa test (K=0.792) and was considered substantial or high. The rectal biopsy showed to be promising and can be used by practitioners, together with other techniques, for antemortem diagnosis in buffalo herds suspected to be affected by paratuberculosis. Samples obtained by rectal biopsy were suitable for ZN staining and real time PCR. Rectal biopsy can be used as a tool for diagnosis and control of Johne´s disease in buffaloes to select and eliminate positive animals within the herd and decrease gradually the spread of Map.

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Diagnóstico de paratuberculose por biópsia retal em búfalos

et al. 2015

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“…Buergelt & Ginn (2000) realizaram a biópsia para diagnosticar a paratuberculose em ruminantes selvagens mantidos em zoológicos. Além disso, no Brasil, biópsia de tecido retal foi realizada em bubalino, cujo tecido revelou imunomarcação para M. avium (Dalto et al 2012). Ainda, vale ressaltar que, em nosso estudo, não foram observadas alterações histoló-gicas no cólon das vacas assintomáticas estudadas.…”

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Diagnóstico imuno-histopatológico da paratuberculose subclínica em bovinos no estado do Rio de Janeiro

et al. 2013

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RESUMO.-O diagnóstico precoce e específico da paratuberculose ainda é um desafio. Isto pode estar associado à baixa sensibilidade dos testes laboratoriais e ou à variação da resposta imunológica frente à infecção por Mycobacterium avium subsp. paratuberculosis. The early and specific diagnosis of paratuberculosis remains a challenge due to the low sensitivity of the currently available laboratory tests and also because of variations in the immune response towards infection with Mycobacterium avium subsp. paratuberculosis. Globally this disease causes significant economic losses, primarily in dairy cattle, owing to the chronic nature of the infection. Paratuberculosis has been described in a number of Brazilian states and from a diversity of domestic ruminant species clearly demonstrating that the disease is present in the country and highlighting the requirement for the development of diagnostic techniques for confirmation of infection and for epidemiological analyses. The aim of this study was to characterize the anatomo-histopathological and immunohistochemical findings in the bowel and mesenteric lymph nodes of assymptomatic cattle, derived from paratuberculosis positive herds located in state of Rio de Janeiro, Brazil. Macroscopic examination during necropsy revealed nonspecific changes including reddening of the gut mucosa, increased volumes for the Peyer's patches and mesenteric lymph nodes and in some case dilation and whitening of the mesenteric lymphatic vessel. Histopathology revealed granulomatous infiltration, occasionally with the formation of giant cells in the jejunal and ileal mucosa or sub-mucosa, and/or in the cortical region of the mesenteric lymph nodes, in 32 of the 52 cattle examined. Tissue sections from these animals were subjected to Ziehl-Neelsen staining, but the presence of acid-fast bacilli was not observed. Subsequent analysis, employing genus specific immunohistochemisty for Mycobacterium, revealed areas of immunoreactivity in sections prepared from a total of six animals. The results of this investigation highlighted the value of histopathology and particularly immunohistochemistry as tools for the diagnosis of subclinical paratuberculosis.

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Paratuberculosis in buffaloes in Northeast Brazil

Brito

Belo-Reis

Barbosa

et al. 2016

Trop Anim Health Prod

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Several farms in the Northeast of Brazil were investigated for Mycobacterium avium subsp. paratuberculosis infection in order to identify the occurrence of paratuberculosis in buffaloes. Samples were obtained from 17 farms, two slaughter houses, and a quarantine area in the Northeast. About 15,000 buffaloes of the Murrah, Mediterranean, and Jafarabadi breed as well as their crossbreeds were evaluated for meat, dairy, and mixed farms with semi-intensive or extensive breeding practices. For diagnostic purposes, postmortem and histopathological examination, including Ziehl-Neelsen test of fecal smears and scraped intestinal mucosa were performed. PCR was applied for fecal samples, mesenteric lymph nodes, and intestines. Six Johne's disease-positive farms, which together with those previously identified, indicate that the disease is spread through the Brazilian Northeast, similar to what occurs in cattle herds in other regions of the country. The increase in prevalence of paratuberculosis is a consequence of introduction of animals from other regions without adequate veterinary assistance and due to the little official attention paid to this initially silent and chronic disease.

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Clinical and pathological insights into Johne′s disease in buffaloes

Cited by 20 publications

References 17 publications

Diagnóstico de paratuberculose por biópsia retal em búfalos

Diagnóstico de paratuberculose por biópsia retal em búfalos

Diagnóstico imuno-histopatológico da paratuberculose subclínica em bovinos no estado do Rio de Janeiro

Paratuberculosis in buffaloes in Northeast Brazil

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