Clinical and Pathological Aspects of Experimental Oleander (Nerium oleander) Toxicosis in Sheep

Aslani, Mohammad Reza; Movassaghi, Ahmad Reza; Mohri, Mehrdad; Abbasian, Ahmad Reza; Zarehpour, M.

doi:10.1023/b:verc.0000042870.30142.56

Cited by 54 publications

(61 citation statements)

References 4 publications

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“…leaves extract. Infiltration of mononuclear inflammatory cells into the portal space with scattered necrosis of hepatocytes in H&E stained sections was registered with an oral administration of dried oleander leaves (110 mg/kg) [46, 62]. Maximum recruitment of ED1 + cells was noted after 24 h of N.O.…”

Section: Discussionmentioning

confidence: 99%

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Effect ofNerium oleander(N.O.) Leaves Extract on Serumhepcidin, Total Iron, and Infiltration of ED1 Positive Cells in Albino Rat

Abbasi

Fatima

Naz

et al. 2013

BioMed Research International

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To gain insight into the hepatohistological alterations in noninjured rat liver, Nerium oleander (N.O.) leaves extract was injected intramuscularly to induce an acute phase reaction (APR). Histopathological changes were studied after 3, 12, and 24 h time course of sterile muscle abscess. Tissue integrity and any infiltration of inflammatory cells in the liver were investigated by Hematoxylin and Eosin and ED1 peroxidase stainings. The administration of N.O. leaves extract (10 mL/kg) in H & E stained sections showed a general vacuolization of cytoplasm resulting loss of polarity with prominent nucleoli after 3 h of induction. At 12 h, eccentric nuclei were also observed in the sections. Marked infiltration of leucocytes with predominate macrophages was also found after 24 h as seen by ED1 positive staining. In the present study, a possible relationship between serum hepcidin and total iron level was also investigated in vivo. An early increase of hepcidin and total iron level (3 h) with a maximum at 12 h (P < 0.01; P < 0.001) was observed. These changes indicate that sterile muscle abscess may induce APR resulting in hepatic damage which is evident with the recruitment of inflammatory cells into the organ.

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Section: Discussionmentioning

confidence: 99%

“…All parts of oleander are toxic containing oleandrin, oleandrigenin, and other cardiac glycosides [45]. Toxic exposure of humans and different species of domestic animals to N. oleander cardenolides occurs commonly throughout the geographic regions where this plant grows [46, 47]. N.O.…”

Section: Introductionmentioning

confidence: 99%

Effect ofNerium oleander(N.O.) Leaves Extract on Serumhepcidin, Total Iron, and Infiltration of ED1 Positive Cells in Albino Rat

Abbasi

Fatima

Naz

et al. 2013

BioMed Research International

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“…Efforts have been made by few investigators to evaluate the toxicological aspects of various parts of Nerium species on few organisms-fish (Tiwari and Singh 2003, 2005Singh and Singh 2002), mammals (Oryan et al 1996;Longford and Boor 1996;Ada et al 2001;Haeba et al 2002;Aslani et al 2004;Saravanan et al 2004;Soto-Blanco et al 2006;Barbosa et al 2007), insects (el-Shazly et al 1996), and molluscs (Singh et al1993;Panigrahi and Raut 1994;Wang et al 2006).…”

Section: Introductionmentioning

confidence: 97%

Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

Prasad

Kumar

Mishra

et al. 2010

Fish Physiol Biochem

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The present study aimed at investigating the effects of Nerium indicum leaf extract on the blood electrolytes of Heteropneustes fossilis for short- and long term. Fish were subjected to Nerium indicum leaf extract for short term (11.27 mg/L i.e. 0.8 of 96 h LC₅₀) and long term (2.81 mg/L i.e. 0.2 of 96 h LC₅₀). Fish were killed on each time intervals from control and experimental (Nerium indicum) groups after 24, 48, 72, and 96 h in short-term exposure and after 7, 14, 21, and 28 days in long-term experiment. Blood samples were analyzed for calcium and inorganic phosphate levels. Acute exposure of Nerium indicum leaf extract caused a progressive decrease in the serum calcium levels after 48 h in fish H. fossilis, which persists till the close of the experiment (96 h). The serum inorganic phosphate levels remain unaffected till 48 h in the Nerium indicum leaf extract-exposed fish. After 72 and 96 h, the levels exhibit a decrease. Chronic Nerium indicum leaf extract treatment provoked a decrease in serum calcium levels at day 14. This decrease continues till 28 days. The serum phosphate level of the Nerium indicum leaf extract-treated fish decreases on day 14 and 21. However, on day 28, the levels become close to the normal values. We conclude that Nerium indicum leaf extract exposure alters the blood electrolytes of the fish, thus causing physiological disturbances which might affect seriously the normal vital functions, growth rate, reproduction, and their survival in nature.

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“…One plant that is found in some CAMS is oleander, which is extremely toxic and has produced deaths and poisoning in various animal species (Aslani et al, 2004;Woods et al, 2004;Haeba et al, 2002;Meyer et al, 1993;Galey et al, 1996;Eddleston et al, 2000b). However, consuming honey from oleander nectar and taking homeopathic medicines to curb binge eating, for example, allow many products to be sold as diet aids.…”

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confidence: 95%

OLeandrin Produces Changes in Intracellular Calcium Levels in Isolated Cardiomyocytes: A Real-Time Fluorescence Imaging Study Comparing Adult to Neonatal Cardiomyocytes

Poindexter¹,

Feng²,

Dasgupta³

et al. 2007

Journal of Toxicology and Environmental Health, Part A

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Oleanders are common, hardy shrubs that grow throughout the southern United States. They contain cardiotonic steroids formed from cardenolides and bufadienolides, making the plant poisonous to both animals and humans. Aliquots of both commercially available oleander and fresh oleander extracts were prepared. Fresh, rod-like, calcium-tolerant adult rat cardiomyocytes and cultured neonatal cardiomyocytes were isolated and treated with 0-4 ng/ml of both preparations, challenged with verapamil and ouabain, and real-time spectrophotometric calcium transients and images were acquired. A number of effects were observed with the adult cells: (1) intracellular calcium levels were increased in a concentration-dependent manner: (2) reduced calcium transient heights and eventual cessation of beating resulted; and (3) increased sparking intensity led to subsequent beating and eventual calcium overload. In the spontaneously beating cultured neonatal myocytes increased intramyocytic calcium levels were also seen, with retention of this calcium rise leading to overload and, as in the adult myocytes, cessation of beating. These observations demonstrate that oleander extract is markedly potent with respect to the elevation of calcium concentrations in cardiomyocytes, and that the inability of the cardiomyocytes to release the accumulated calcium possibly indicates a role for oleandrin in inhibition of ryanodine receptor calcium release channels, calcium uptake via Na+,K+-ATPase inhibition [EC 3.6.1.3], and/or dysfunction of sarcolemmal calcium release channels.

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Clinical and Pathological Aspects of Experimental Oleander (Nerium oleander) Toxicosis in Sheep

Cited by 54 publications

References 4 publications

Effect ofNerium oleander(N.O.) Leaves Extract on Serumhepcidin, Total Iron, and Infiltration of ED1 Positive Cells in Albino Rat

Effect ofNerium oleander(N.O.) Leaves Extract on Serumhepcidin, Total Iron, and Infiltration of ED1 Positive Cells in Albino Rat

Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

OLeandrin Produces Changes in Intracellular Calcium Levels in Isolated Cardiomyocytes: A Real-Time Fluorescence Imaging Study Comparing Adult to Neonatal Cardiomyocytes

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