Clinical and pathologic prognostic indicators in colorectal cancer. A population‐based study

Leòn, Maurizio Ponz de; Sant, Milena; Micheli, Andrea; Sacchetti, C; Gregorio, Carmela Di; Fante, Rossella; Zanghieri, Gianni; Melotti, Gianluigi; Gatta, Gemma

doi:10.1002/1097-0142(19920201)69:3<626::aid-cncr2820690305>3.0.co;2-#

Cited by 91 publications

(48 citation statements)

References 41 publications

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“…They were defined as proximal to the splenic flexure (Ponz de Leon et al, 1992). Eight cases were excluded (three men and five women) since DNA amplification was not possible.…”

Section: Patients and Samplesmentioning

confidence: 99%

Microsatellite instability and intratumoural heterogeneity in 100 right-sided sporadic colon carcinomas

et al. 2002

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Microsatellite instability has been proposed as an alternative pathway of colorectal carcinogenesis. The aim of this study was to evaluate the interest of immunohistochemistry as a new tool for highlighting mismatch repair deficiency and to compare the results with a PCR-based microsatellite assay. A total of 100 sporadic proximal colon adenocarcinomas were analysed. The expression of hMLH1, hMSH2 and hMSH6 proteins evaluated by immunohistochemistry was altered in 39% of the cancers, whereas microsatellite instability assessed by PCR was detected in 43%. There was discordance between the two methods in eight cases. After further analyses performed on other tumoural areas for these eight cases, total concordance between the two techniques was observed (Kappa=100%). Our results demonstrate that immunohistochemistry may be as efficient as microsatellite amplification in the detection of unstable phenotype provided that at least two samples of each carcinoma are screened, because of intratumoural heterogeneity.

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“…They were defined as proximal to the splenic flexure (Ponz de Leon et al, 1992). Eight cases were excluded (three men and five women) since DNA amplification was not possible.…”

Section: Patients and Samplesmentioning

confidence: 99%

Microsatellite instability and intratumoural heterogeneity in 100 right-sided sporadic colon carcinomas

et al. 2002

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“…Grading was carried out according to the World Health Organization histological classification system (Jass and Sobin, 1989 (Turnbull et al, 1967). Tumours occurring from the caecum to the splenic flexure were considered proximal (49, 25%), and those from the descending colon to the rectum distal (146, 75%) (Ponz de Leon et al, 1992).…”

Section: Patientsmentioning

confidence: 99%

Loss of heterozygosity at 18q21 is indicative of recurrence and therefore poor prognosis in a subset of colorectal cancers

et al. 1999

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Summary Adjuvant therapies are increasingly used in colorectal cancers for the prevention of recurrence. These therapies have side-effects and should, thus, be used only if really beneficial. However, the development of recurrence cannot be predicted reliably at the moment of diagnosis, and targeting of adjuvant therapies is thus based only on the primary stage of the cancer. Loss of heterozygosity (LOH) in the long arm of chromosome 18 is suggested to be related to poor survival and possibly to the development of metastases. We studied the value of LOH at 18q21 as a marker of colorectal cancer prognosis, association with clinicopathological variables, tumour recurrence and survival of the patients. Of the 255 patients studied, 195 were informative as regards LOH status when analysed in primary colorectal cancer specimens using the polymerase chain reaction (PCR) and fragment analysis. LOH at 18q21 was significantly associated with the development of recurrence (P = 0.01) and indicated poor survival in patients of Dukes' classes B and C, in which most recurrences (82%) occurred. An increased rate of tumour recurrence is the reason for poor survival among patients with LOH at 18q21 in primary cancer. These patients are a possible target group for recurrence-preventing adjuvant therapies.

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“…For most carcinomas, such as colorectal carcinomas, an increased amount of matrix is an unfavorable prognostic factor. 1 Hyaluronan is a polysaccharide that is important during rapid tissue growth, i.e., during embryogenesis, wound healing and tumorigenesis. 2 Hyaluronan affects normal physiology by modulating tissue hydration and osmotic balance.…”

mentioning

confidence: 99%

Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors

Jacobson

Rahmanian

Rubin

et al. 2002

Intl Journal of Cancer

112

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Advanced colorectal cancers are often associated with elevated amounts of hyaluronan. To investigate the importance of hyaluronan in colon carcinoma tumor progression, we have expressed by stable transfection hyaluronan synthase 2 (Has2) and hyaluronidase 1 (Hyal1) in the rat colon carcinoma cell line, PROb. We found that hyaluronan overproduction led to a higher growth rate of tumor cells in vitro, and to a faster development of transplantable tumors in syngeneic rats, compared to the mock-transfectants. Has2 transfected PROb cells gave rise to tumors that were significantly less vascularized, but had a significantly larger viable tumor fraction compared to tumors generated from mocktransfectants. In contrast, Hyal1 overexpression suppressed the growth rate of tumor cells both in vitro and in vivo. Moreover, tumors derived from Hyal1-transfected cells had a significantly larger necrotic area than tumors derived from mock-and Has2-transfectants. Our study demonstrates that Has2 overproduction promotes tumorigenicity, whereas Hyal1 overexpression suppresses tumorigenicity in an experimental model for colon carcinoma.

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Clinical and pathologic prognostic indicators in colorectal cancer. A population‐based study

Cited by 91 publications

References 41 publications

Microsatellite instability and intratumoural heterogeneity in 100 right-sided sporadic colon carcinomas

Microsatellite instability and intratumoural heterogeneity in 100 right-sided sporadic colon carcinomas

Loss of heterozygosity at 18q21 is indicative of recurrence and therefore poor prognosis in a subset of colorectal cancers

Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors

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