Clinical and paraclinical changes in experimental colisepticemia in neonatal calves

Abstract

The present study aim was to assess changes in clinical signs and plasma cytokines in calves experimentally infected with Escherchia coli and use them as a tool to diagnose colisepticemia. Ten healthy Holstein-Frisian bull calves were used for this experimental study. Experimental colisepticemia was induced in calves with intravenous injection of 1.5 × 109 CFU of O111:H8 strain of E.coli. Clinical scores were recorded before induction of septicemia, every 30 minutes for 8 hours, every hour from 8 till 12 h, every 3 hours from 12 till 24 hours after bacterial challenge. Blood samples were collected to determine plasma concentration of tumor necrosis factor-α (TNF-α) and gamma interferon gamma (IFN-γ) before and after E.coli IV injection. Blood culture was performed before and after bacterial challenge to confirm colisepticemia. The present study showed that total clinical score of the calves increased with a simultaneous significant rise in plasma concentration of TNF-α and INF-γ during septicemia period (P< 0.05). Changes in the heart and respiratory rate during septicemia and using clinical scoring are not enough to assess the magnitude of infection and disease progress; therefore, it is recommended some laboratory tests be used for better evaluation of clinical status of the septic calves

Effect of Vitamin C on the Immune System in Cattle Immunized With Blackleg Vaccine

“…There has been some suggestion that vitamin C is effective Many studies have demonstrated the association between vitamin C and antibody levels in cattle. It has been reported that when the calves received colostrum are used orally 3 grams a day in the first week, 2 grams in the second week, and 1 gram in the third week, the amount of serum gamma globulin is increased significantly on the 14th day but not significantly on the 28th day (Lotfollahzadeh et al, 2005). In a study, vitamin C administered to calves was found to not affect the amount of total plasma IgG (Hidiroglou et al, 1995).…”

“…The amount of IgG measured on days 14 and 28 in the vaccine-vitamin group was found to have increased significantly (P<0.05) compared to the vaccine group. It has been reported that vitamin C increases the amount of serum antibody in newborn calves (Lotfollahzadeh et al, 2005), calves vaccinated against Histophilus somni (Otomaru et al, 2021), in beef cattle vaccinated against Foot and Mouth disease (Kızıl & Gül, 2010), in dairy cattle with mastitis (Chaiyotwittayakun et al, 2002), in goats vaccinated with Brucella melitensis (İmik et al, 2000), chickens vaccinated against Newcastle virus (Gouda et al, 2020;Khan et al, 2014;Mirfendereski & Jahanian, 2015;Toplu et al, 2014), in chickens vaccinated against Infectious Bronchitis (Khan et al, 2014), in chickens vaccinated against Infectious Bursal Disease ( Wu et al, 2000), when injected into embryonated eggs (Zhu et al, 2019), in human lymphocytes in vitro (Tanaka et al, 1994), in mice vaccinated with Foot and Mount Disease (Wu et al, 2018). The results obtained in this study are consistent with the research results presented above.…”

“…In this country, cattle over four months old are vaccinated against the disease before they go to pasture. The vaccine is given behind the shoulder and subcutaneously (Mamak et al, 2018).…”