Clinical and Microbiology Profile and Outcome of Diarrhea by Coccidian Parasites in Immunocompetent Children

Iyer, Ranganathan; Rao, Jangam Rekha; Venkatalakshmi, A.; Nahdi, Faisal B.

doi:10.1097/inf.0000000000000770

Cited by 4 publications

(1 citation statement)

References 17 publications

(23 reference statements)

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“…Stool samples were concentrated by Sheather's sucrose floatation technique. 15 Modified Ziehl-Neelsen staining of stool smears was performed using 1% concentrated sulphuric acid and observed microscopically for the presence of oocysts of C. cayetanensis. 16…”

Section: Microscopic Examination For Oocysts Of C Cayetanensismentioning

confidence: 99%

A new single run polymerase chain reaction assay for cyclosporiasis in immunocompromised patients

Katiyar¹,

Gulati

Parameswaran³

et al. 2021

IJMMTD

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causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen in people living with HIV/AIDS (PLHA), post-renal transplant (PRT) patients and immunocompromised children (IC). Diagnostic microscopy for the oocysts of the parasite is less sensitive, requiring examination of multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay for the detection of and it was used to know the hospital based prevalence of cyclosporiasis. A cross-sectional study was conducted from June 2016 to October 2020 in a tertiary care teaching hospital. A new single run amplification PCR-based diagnostic assay was developed for . Stool samples were collected from 121 PLHA, 135 PRT and 79 immunocompromised children (IC) other than PLHA and PRT. All stool samples were examined for the presence of oocysts as well as tested with new PCR assay. Modified Ziehl-Neelsen staining of the concentrated stool smear did not reveal oocysts of species in any stool specimen. However, new PCR assay detected in 2 stool specimens – one from a PLHA patient and another from a PRT patient, giving a prevalence of 0.6% (2/335), 0.8% (1/121) in PLHA and 0.7% (1/135) in PRT. It was not detected in IC. Cyclosporiasis is infrequent in southern part of India. The new single run PCR assay developed by us is simple and cost effective molecular assay for the detection of .

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Section: Microscopic Examination For Oocysts Of C Cayetanensismentioning

confidence: 99%