Clinical and microbiological characteristics of OXA-23- and OXA-143-producing Acinetobacter baumannii in ICU patients at a teaching hospital, Brazil

Neves, Francelli Cordeiro; Clemente, Wanessa T.; Lincopán, Nilton; Paião, Isabela Dias; Neves, Patrícia; Romanelli, Roberta Maia de Castro; Lima, Swiany Silveira; Paiva, Luciene F.; Mourão, Paulo Henrique Orlandi; Nobre-Junior, Vandack A.

doi:10.1016/j.bjid.2016.08.004

Cited by 27 publications

(14 citation statements)

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“…(55) reported a frequency of 41.7% for non-baumannii . We did not find the bla OXA-143-like gene and the studies report absence (54), low (56) and high frequencies for this carbapenemase gene (52). Some other studies show null or low prevalences of bla OXA-58-like (31, 57) and bla OXA-72-like (54, 58), while they were not identified in the present study.…”

Section: Discussioncontrasting

confidence: 75%

Multidrug-resistant Gram-negative bacteria from patients, hospital environment and healthcare workers: a six-month cross-sectional study

Sereia

Cunha

Tartari

et al. 2018

Preprint

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19Healthcare-associated infections (HAI) are an important public health threat with the 20 multidrug-resistant (MDR) gram-negative bacteria (GNB) being of particular concern. Here 21 we present the antimicrobial resistance profile of HAI-related GNB (HAIrB) isolated from 22 patients (PT), healthcare workers (HCW) and hospital environment (HE) in a six-month 23 screening program. From the 180 sampling points distributed in six hospital units, a total of 24 1,080 swabs were collected allowing the isolation of 390 HAIrB: 50.5% from HE, 42.6% 25 2 from PT and 6.9% from HCW. Among the HAIrB, 32.6% were characterized as MDR and 26 38.7% as extended-spectrum cephalosporins resistant (ESC-R), showing no differences in the 27 distribution between PT, HE and HCW. Carbapenem resistance (CARB-R) was detected for 28 17.7% of all HAIrB, being higher among Acinetobacter spp. isolates (36.5%), followed by 29 Enterobacteriaceae (14.5%) and Pseudomonas spp. (11.8%). Except for the ICU, that 30 revealed higher MDR, CARB-R and ESC-R rates, HAIrB-resistant profiles were similarly 31 detected within the hospital units. Prevalence of bla KPC-like and bla CTX-M-1 β-lactamases-32 resistance genes was higher in K. pneumoniae and E. cloacae complex, while bla OXA-23-like 33 and bla SPM-like were higher in A. baumannii and P. aeruginosa, respectively. This study 34 reveals that the spreading of HAIrB within a hospital environment is higher than predicted, 35indicating that healthcare workers, hospital areas and equipment are key players on 36 dissemination of MDR gram-negative bacteria and shows that an active surveillance program 37 can provide precise understanding and direct actions towards control of HAI. 38 39 3 14). In this scenario, adoption of surveillance programs based on new technologies associated 51 with the rational management of antimicrobials and the continuous training for healthcare 52 workers can allow an effective control of HAI transmission, ensuring the patient safety and a 53 consequent reduction of direct and indirect healthcare costs (5,(15)(16). 54To better understand the antimicrobial resistance profile and the dissemination of HAI-related 55 GNB in the healthcare setting we carried out a six-month surveillance program targeting 56 patients, hospital environment and healthcare workers. 57 58 Material and Methods 59 Study design 60The Healthcare-associated Infections Microbiome Project (HAIMP) was carried out at the 61 Professor Polydoro Ernani

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Section: Discussioncontrasting

confidence: 75%

Multidrug-resistant Gram-negative bacteria from patients, hospital environment and healthcare workers: a six-month cross-sectional study

Sereia

Cunha

Tartari

et al. 2018

Preprint

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“…OXA-143 enzyme (Class D carbapenem-hydrolyzing enzyme) in Acinetobacter baumannii was firstly described by Higgins, Poirel [37]. Furthermore, Neves, Clemente [38] found that OXA-23 was the most common acquired cabapenemase (51.2%) followed by OXA-143 (18.6%) among carbapenem- resistant Acinetobacter baumanii which confirm their important role in carbapenem resistance. In this study, OXA-143 was the most common followed by OXA-23.…”

Section: Discussionmentioning

confidence: 78%

Antimicrobial resistance pattern and molecular epidemiology of ESBL and MBL producing Acinetobacter baumannii isolated from hospitals in Minia, Egypt

El-Baky

Farhan

Ibrahim

et al. 2020

Alexandria Journal of Medicine

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Introduction: Multidrug resistant (MDR) Acinetobacter baumanii (A. baumannii) strains have emerged as novel nosocomial pathogens threatening patients' lives, especially in intensivecare units (ICUs). This study aims to determine the prevalence of carbapenemase genes and CTX-M-15 and the resistance pattern of carbapenemase producing isolates. Methods: A total of 530 clinical specimens were collected from patients suffering from different infections, antibiotic susceptibility test was performed using kirby-bauer disk diffusion method. ESβL production was detected phenotypically by double-disc synergy test (DDST). Carbapenemase production was tested by Modified Hodge Test (MHT). Then, these isolates were tested for MBL detection by disc potentiation test. Carbapenemase encoding genes (VIM, IMP, GIM and SPM, OXA-51, OXA-23 and OXA-143) and CTX-M-15 were tested by polymerase chain reaction (PCR). Results: Out of 530 samples, 20 bacterial isolates were identified as A. baumannii from different infectious cases, 35% of isolates were ESBL-producers. Eleven isolates were resistant to imipenem (4 isolates) and meropenem (7 isolates). All carbapenem resistant isolates were MHT positive. Nine (45%) isolates were confirmed as A. baumannii by OXA-51 (all were carbapenem resistant). Distribution of IMP, VIM, GIM and SPM, OXA-23, OXA-143 and CTX-M-15 by PCR were 55, 50, 50, 25, 35, 45 and 33% respectively. Conclusion: The high prevalence of resistance genes and the resistance pattern of the isolates indicate that the detection of ESBLs and MBLs phenotypically and genotypically with the study of the resistance pattern of the isolates is critically important for the surveillance of drug resistance in the hospital environment.

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“…Further identified variants of the OXA-143 group include OXA-182, OXA-231, OXA-253, and OXA-255. OXA-143 is still detected in Brazil [157,158], and, although believed that this country is the major epicenter, OXA-143 was detected in 2017 for the first time in 14% of A. baumannii isolates from teaching hospitals in the central part of Iran [159]. The variant OXA-231 was also isolated from a nosocomial A. baumannii isolate recovered from urine of a female patient, at Londrina, Brazil.…”

Section: Ambler Class D Carbapenemases (Oxacillinases)mentioning

confidence: 99%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

2020

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Carbapenemases are β-lactamases belonging to different Ambler classes (A, B, D) and can be encoded by both chromosomal and plasmid-mediated genes. These enzymes represent the most potent β-lactamases, which hydrolyze a broad variety of β-lactams, including carbapenems, cephalosporins, penicillin, and aztreonam. The major issues associated with carbapenemase production are clinical due to compromising the activity of the last resort antibiotics used for treating serious infections, and epidemiological due to their dissemination into various bacteria across almost all geographic regions. Carbapenemase-producing Enterobacteriaceae have received more attention upon their first report in the early 1990s. Currently, there is increased awareness of the impact of nonfermenting bacteria, such as Acinetobacter baumannii and Pseudomonas aeruginosa, as well as other Gram-negative bacteria that are carbapenemase-producers. Outside the scope of clinical importance, carbapenemases are also detected in bacteria from environmental and zoonotic niches, which raises greater concerns over their prevalence, and the need for public health measures to control consequences of their propagation. The aims of the current review are to define and categorize the different families of carbapenemases, and to overview the main lines of their spread across different bacterial groups.

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Clinical and microbiological characteristics of OXA-23- and OXA-143-producing Acinetobacter baumannii in ICU patients at a teaching hospital, Brazil

Abstract: In this study, clonally unrelated OXA-123- and OXA-143-producing A. baumannii strains in ICU patients were strongly correlated to colonization with infected patients being associated with a poor outcome.

Cited by 27 publications

References 23 publications

Multidrug-resistant Gram-negative bacteria from patients, hospital environment and healthcare workers: a six-month cross-sectional study

Multidrug-resistant Gram-negative bacteria from patients, hospital environment and healthcare workers: a six-month cross-sectional study

Antimicrobial resistance pattern and molecular epidemiology of ESBL and MBL producing Acinetobacter baumannii isolated from hospitals in Minia, Egypt

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

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