Clinical and Laboratory Studies of Mumps

Utz, John P.; Kasel, Julius A.; Cramblett, Henry G.; Szwed, Clarence F.; Parrott, Robert H.

doi:10.1056/nejm195709122571103

Cited by 52 publications

(11 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in previously vaccinated individuals who become infected with mumps, the timing and duration of the IgM response are much more variable or nonexistent. In a nonvaccinated individual, mumps virus RNA can be detected in saliva and urine samples for up to 13 days after the onset of symptoms (16,23,24).…”

mentioning

confidence: 99%

Real-Time Reverse Transcription-PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens

et al. 2007

View full text Add to dashboard Cite

The mumps virus is a negative-strand RNA virus in the family Paramyxoviridae. Mumps infection results in an acute illness with symptoms including fever, headache, and myalgia, followed by swelling of the salivary glands. Complications of mumps can include meningitis, deafness, pancreatitis, orchitis, and first-trimester abortion. Laboratory confirmation of mumps infection can be made by the detection of immunoglobulin M-specific antibodies to mumps virus in acute-phase serum samples, the isolation of mumps virus in cell culture, or by detection of the RNA of the mumps virus by reverse transcription (RT)-PCR. We developed and validated a multiplex real-time RT-PCR assay for rapid mumps diagnosis in a clinical setting. This assay used oligonucleotide primers and a TaqMan probe targeting the mumps SH gene, as well as primers and a probe that targeted the human RNase P gene to assess the presence of PCR inhibitors and as a measure of specimen quality. The test was specific, since it did not amplify a product from near-neighbor viruses, as well as sensitive and accurate. Real-time RT-PCR results showed 100% correlation with results from viral culture, the gold standard for mumps diagnostic testing. Assay efficiency was over 90% and displayed good precision after performing inter-and intraassay replicates. Thus, we have developed and validated a molecular method for rapidly diagnosing mumps infection that may be used to complement existing techniques.

show abstract

mentioning

confidence: 99%

Real-Time Reverse Transcription-PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Mumps virus has been recovered from the saliva and urine (Utz et al, 1957), thyroid (Eylan et al, 1957), and cerebrospinal fluid (Lavergne et al, 1937), and was found in the blood by Woolstein (1918). We can find no reference to isolation from the joint.…”

Section: Discussionmentioning

confidence: 99%

Mumps Arthritis

Lass¹,

Shephard²

1961

BMJ

View full text Add to dashboard Cite

“…There are very few reports of the isolation of any virus from urine but one feels that this may simply reflect the fact that people so far have seen no logical reason for looking there. The closely related virus of mumps can certainly be isolated from this source (Utz et al, 1957). However, attempts to isolate SV-5 from any human source will be frustrated by the presence of the same virus in the very tissue used for the purpose.…”

Section: Discussionmentioning

confidence: 99%

Haemadsorption by Uninoculated Cultures of Monkey Kidney Epithelium

White

1962

Aust J Exp Biol Med

View full text Add to dashboard Cite

SUMMARY A high proportion of the batches of monkey kidney cells commercially available in Australia carry a haemadsorbing virus, the properties of which are described. The virus appears to be identical with or very closely related to SV‐5. Since diagnostic laboratories usually rely on the haemadsorption technique for the isolation of the viruses of para‐influenza, influenza and mumps from the respiratory tract of man and animals, the presence of this agent may seriously complicate the interpretation of results. The problem can be partially overcome by the routine incorporation of SV‐5 antiserum in cell culture media. However, since SV‐5 appears also to cause human infection, it is not practicable to try to isolate this virus from man in tissue which already contains it. For this and other reasons discussed it is suggested that monkey kidney can no longer be considered the tissue of choice for the isolation of respiratory viruses and should be replaced by another cell type.

show abstract

Clinical and Laboratory Studies of Mumps

Cited by 52 publications

References 6 publications

Real-Time Reverse Transcription-PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens

Real-Time Reverse Transcription-PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens

Mumps Arthritis

Haemadsorption by Uninoculated Cultures of Monkey Kidney Epithelium

Contact Info

Product

Resources

About