Clinical and In Silico Outcomes of the Expression of miR-130a-5p and miR-615-3p in Tumor Compared with Non-Tumor Adjacent Tissues of Patients with BC

Ilkhani, Khandan; Delgir, Soheila; Safi, Asma; Seif, Farhad; Samei, Azam; Bastami, Milad; Alivand, Mohammad Reza

doi:10.2174/1871520620666200924105352

Cited by 7 publications

(6 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiRNAs have been demonstrated as promising biomarkers for diagnosis and prognosis of BC [ 38 , 39 ]. Irregular expression of miRNAs impacts the processes involved in BC development such as invasion, metastasis, promoting tissue, stimulating anti-apoptotic activity, drug resistance, and metabolism reprogramming [ 19 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

The expression of miR-513c and miR-3163 was downregulated in tumor tissues compared with normal adjacent tissue of patients with breast cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Breast cancer (BC) is the most invasive cancer with different subtypes that its metabolism is unique compared with normal cells. Glutamine is considered critical nutrition that many cancer cells, particularly BC cells, are dependent on it for growth and proliferation. Therefore, targeting glutamine metabolism, especially enzymes that are related to this pathway, can be beneficial to design anti-cancer agents. Recent evidence has shown that microRNAs (miRNAs), with a short length and single-strand properties, play a prominent role in regulating the genes related to glutamine metabolism, which may control the development of cancer. Methods In silico analysis confirmed that miR-513c and miR-3163 might be involved in glutamine metabolism. The expression level of these two miRNAs was evaluated in eighty BC tissues and normal adjacent tissues. Furthermore, GSE38167, GSE38867, GSE42128, GSE45666, and GSE53179 were employed from gene expression omnibus (GEO). The Limma package was utilized to identify differentially expressed miRNAs (DEMs) of mentioned datasets to evaluate miR-513c and miR-3163 expression. Further, in silico analysis was utilized to predict the potential biological processes and molecular pathways of miR-513c and miR-3163, based on their target genes. Results In silico studies revealed top categories of biological processes and cellular pathways that might play a critical role in metabolism reprogramming and cancer development and were target genes for miR-513c and miR-3163. The current study showed that miR-513c (p value = 0.02062 and FC = − 2.3801) and miR-3163 (p value = 0.02034 and FC = − 2.3792) were downregulated in tumor tissues compared to normal adjacent tissues. The analysis of GEO microarray datasets showed that miR-513c was downregulated in GSE38167, GSE38867, GSE42128, GSE45666 and GSE53179, whereas there was a significant downregulation of miR-3163 in only two studies, including GSE38867 and GSE42128 that they were in accordance with our experimental results. Furthermore, the subgroup analysis did not show any substantial relationship between expression levels of these two miRNAs and factors such as age, family history of cancer, and abortion history. Conclusion MiR-513c and miR-3163 were downregulated in BC tissues, which might serve as tumor suppressors. They are suggested as potential therapeutic targets for patients with BC.

show abstract

Section: Discussionmentioning

confidence: 99%

The expression of miR-513c and miR-3163 was downregulated in tumor tissues compared with normal adjacent tissue of patients with breast cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to studies performed on miRNAs, deregulation of miRNAs, change the expression of tumor suppressors, oncogenes and other genes (20). Given that the expression pro le of miRNAs in BC cells differs from those in non-cancerous cells (21), they can be used as biomarkers in BC diagnosis and prognosis (19,22). Changes in the miRNA expression pro le induce metastasis, tissue invasion, apoptosis deregulation, and drug resistance in BC (5,23,24).…”

Section: Discussionmentioning

confidence: 99%

“…For RNA extraction, we used liquid nitrogen for homogenizing tissue samples and extracted by Trizol reagent and for quality and quantity of extracted RNAs, we used a NanoDrop spectrometer (Thermo Scienti c, USA). After extraction, obtained RNAs were eluted in 50 μL of RNase-free water and stored at -80 °C (19).…”

Section: Experimental Protocolmentioning

confidence: 99%

In Silico and Experimental Outcomes of the Expression of miR508-5p and miR-635 in Tumor Compared with Non-Tumor Adjacent Tissues of Patients with Breast Cancer

Sehrig

Zaefizadeh

Ahmadizadeh

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. Various studies have reported the effect of epigenetic changes, including DNA methylation and microRNAs, on breast carcinogenesis. microRNAs play an important role in the post-transcriptional regulation of genes and are important regulators of oncogenic pathways. Studying microRNAs in BC facilitates the development of targeted therapies and early detection of this cancer. This study aimed to evaluate the expression level of miR-508-5p and miR-635 in BC tumor tissues compared to healthy marginal tissues.Methods and Materials: In silico analysis confirmed microarray datasets (GSE40525, GSE44124 and GSE45666) downloaded from the GEO database. The analysis was defined using the Affy packages in R software to screen remarkably dysregulated miRNAs attended by utilized to predict the potential biological processes and molecular pathways of miR-508-5p and miR-635. Experimental statistical significance of differences in miRNA relative expression results was analyzed by pair-wise fixed reallocation randomization test as a statistical model included in the REST (relative expression software tool).Result: GEO microarray data set, similar to qPCR results, showed that miR-508-5p was downregulated in the sample group by a mean factor of 0.327 (S.E.M range is 0.031-2.000). Moreover, miR-635 was upregulated in the sample group by a mean factor of 2.361 (S.E.M range is 0.250-16.000).Conclusion: MiR-508-5p was downregulated, while miR-635 was upregulated in BC tissues. They may be proposed as diagnostic and therapeutic biomarkers for patients with BC.

show abstract

“…It is involved in many important processes such as cell growth and apoptosis and drug resistance/sensitivity. Thus, targeting PI3K/AKT and its downstream pathways may provide new hope for therapeutic interventions of cancers [ 13 – 16 ]. MicroRNAs (miRNAs) are a family of noncoding-RNAs, playing a crucial role in post-transcriptionally gene expression modulation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

The expression of miRNA-152-3p and miRNA-185 in tumor tissues versus margin tissues of patients with chemo-treated breast cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objective Breast cancer (BC) is the most significant and lethal type of cancer in women. Although there are many newly develop chemotherapy drugs for patients with BC treating at various stages, drug resistance is the most important obstacle in their effectiveness for BC treatment. On the other hand, microRNAs are considered key regulators of genes involved in carcinogenesis and chemoresistance in cancers. The purpose of this study was to evaluate the role of miR-152-3p and miR-185 in intrinsic chemoresistance and proliferation of BC. In addition, the potential role of these miRNAs during chemoresistance was evaluated through possible signaling pathways. Results Here, miR-152-3p was significantly downregulated in tumor tissues compared to the corresponding margin tissues in patients with BC (p-value ≥ 0.04407 and fold change = − 2.0552). In contrast, no statistically significant difference was observed in the miR-185 expression between the two groups. Furthermore, no significant correlation was found between the expression of these two miRNAs and subfactors, including cancer family history, abortion, and age. Downregulation of miR-152-3p could be considered a promising regulator of BC chemoresistance.

show abstract

Clinical and In Silico Outcomes of the Expression of miR-130a-5p and miR-615-3p in Tumor Compared with Non-Tumor Adjacent Tissues of Patients with BC

Cited by 7 publications

References 50 publications

The expression of miR-513c and miR-3163 was downregulated in tumor tissues compared with normal adjacent tissue of patients with breast cancer

The expression of miR-513c and miR-3163 was downregulated in tumor tissues compared with normal adjacent tissue of patients with breast cancer

In Silico and Experimental Outcomes of the Expression of miR508-5p and miR-635 in Tumor Compared with Non-Tumor Adjacent Tissues of Patients with Breast Cancer

The expression of miRNA-152-3p and miRNA-185 in tumor tissues versus margin tissues of patients with chemo-treated breast cancer

Contact Info

Product

Resources

About