Clinical and immunologic changes after allergen immunotherapy with Hop Japanese pollen

Park, Hae‐Sim; Nahm, Dong Ho; Kim, Hee Yeon; Suh, Yoo Jin; Cho, Jae Woong; Kim, Sun Sin; Lee, Soo Keol; Jung, Ji Ye

doi:10.1016/s1081-1206(10)62493-3

Cited by 18 publications

(13 citation statements)

References 19 publications

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“…Symptom scores for asthma in season were reduced after a year of immunotherapy. 38 There have been reports of several individual cases of latex allergy successfully treated with latex extracts. 39,40 Two placebo-controlled studies used standardized latex extracts prepared by 2 European commercial suppliers.…”

Section: New Antigensmentioning

confidence: 99%

Immunotherapy: 1999-2004

Norman

2004

Journal of Allergy and Clinical Immunology

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Section: New Antigensmentioning

confidence: 99%

Immunotherapy: 1999-2004

Norman

2004

Journal of Allergy and Clinical Immunology

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“…Degrees of airway hyperresponsiveness to methacholine were measured out of season before and every year using the method previously described (6). …”

Section: Methodsmentioning

confidence: 99%

“…A change of specific IgE level to Hop J was determined by ELISA according to the previously described method (6). Briefly, microtiter plates (Costar, Corning, NY, U.S.A.) were first coated with 100 µL of Hop J pollen extract (1 µg/well) and left at 4℃ overnight.…”

Section: Methodsmentioning

confidence: 99%

“…12% SDS-PAGE and IgG 4 -immunoblot analysis were performed under reducing conditions according to methods described previously (6). The Hop J pollen extracts were mixed with sample buffer (Tris-HCl 31 mM/L, 10% glycerol, 1% SDS, 0.0025%, bromophenol blue, 2.5% β-mercaptoethanol, pH 6.8) and heated in boiling water for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…It has been considered one of the major causative pollens of autumn pollinosis in this country (5). We reported a preliminary study showing a favorable clinical and immunologic effect after the one-year immunotherapy with Hop J pollen extracts (6). It is essential to extend the study period and observe the mechanism of Hop J pollen immunotherapy.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes of Serum Cytokines After the Long Term Immunotherapy with Japanese Hop Pollen Extracts

Lee

Kim

et al. 2006

J Korean Med Sci

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Japanese hop (Hop J) pollen has been considered as one of the major causative pollen allergens in the autumn season. We developed a new Hop J immunotherapy extract in collaboration with Allergopharma (Reinbeck, Germany) and investigated immunologic mechanisms during 3 yr immunotherapy. Twenty patients (13 asthma with rhinitis and 7 hay fever) were enrolled from Ajou University Hospital. Sera were collected before, 1 yr, and 3 yr after the immunotherapy. Changes of serum specific IgE, IgG1, and IgG4 levels to Hop J pollen extracts and serum IL-10, IL-12, TGFβ1 and soluble CD23 levels were monitored by ELISA. Skin reactivity and airway hyper-responsiveness to methacholine were improved during the study period. Specific IgG1 increased at 1 yr then decreased again at 3 yr, and specific IgG4 levels increased progressively (p<0.05, respectively), whereas total and specific IgE levels showed variable responses with no statistical significance. IL-10, TGF-β1 and soluble CD23 level began to decrease during first year and then further decreased during next two years with statistical significances. (p<0.05, respectively). In conclusion, these findings suggested the favorable effect of long term immunotherapy with Hop J pollen extracts can be explained by lowered IgE affinity and generation of specific IgG4, which may be mediated by IL-10 and TGF-β1.

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