Clinical and Histopathological Outcomes of Subconjunctival Triamcinolone Injection for the Treatment of Acute Ocular Alkali Burn in Rabbits

Saud, Esper Escobar; Moraes, Haroldo Vieira de; Marculino, Leonardo Guedes; Gomes, José Álvaro Pereira; Allodi, Silvana; Miguel, Nádia C O

doi:10.1097/ico.0b013e318221ce99

Cited by 28 publications

(12 citation statements)

References 27 publications

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“…Our results are in agreement with thouse of Saud and colleagues, who showed an improvement in corneal opacity with no corneal perforations in rabbits subjected to alkali burn and subconjunctival injections of the corticosteroid triamcinolone. 27 Several enzymes and proteases, including collagenases, are stored within the cytoplasmic granules of neutrophils. Experiments showed that depletion of neutrophils dramatically improved the fate of alkali burn injuries.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Dexamethasone and Doxycycline on Inflammation and MMP Production in Murine Alkali-Burned Corneas Associated with Dry Eye

et al. 2016

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Alkali burns to the cornea are among the most devastating injuries to the eye. The purpose of this study was to evaluate the effects of dexamethasone (Dex) or doxycycline (Doxy) on protease activity and corneal complications in a combined model (CM) of alkali burn and dry eye. C57BL/6 mice were subjected to the CM for 2 or 5 days (D). Mice were topically treated either with Dex (0.1%), Dox (0.025%) or vehicle QID and observed daily for appearance of corneal perforation. Quantitative real time PCR was performed to measure expression of inflammation cytokines and matrix metalloproteinases (MMP) in whole cornea lysates. No perforations were observed in the Dex-treated corneas. All wounds in Doxy-treated corneas were closed 2D post-injury, and they had significantly lower corneal opacity scores at days 4 and 5 post-injury compared to BSS treatment. Dex-treated corneas had the lowest corneal opacity scores. Dex treatment significantly decreased expression of IL-1β, IL-6, MMPs -1, -9, -13, and TIMP-1 after 2 days but increased levels of MMP-8, while Doxy treatment significantly decreased IL-1β, IL-6, MMP-8, and -9, compared to vehicle. Decreased MMP -1, -9 and -13 immunoreactivity and gelatinolytic activity were seen in corneas treated with Doxy and Dex compared to vehicle. Increased neutrophil infiltration and myeloperoxidase activity was noted in the vehicle group compared to Dex 2 days post-injury. These findings demonstrate that early initiation of anti-inflammatory therapy is very efficacious in preserving corneal clarity and facilitating wound healing, while modulating MMP production and suppressing neutrophil infiltration.

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Section: Discussionmentioning

confidence: 99%

Differential Effects of Dexamethasone and Doxycycline on Inflammation and MMP Production in Murine Alkali-Burned Corneas Associated with Dry Eye

et al. 2016

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“…An alkali-induced corneal model showed that subconjunctival injection of dexamethasone reduced the intensity of inflammation and area of corneal NV. 81 Another study, of a murine suture-induced corneal model, revealed that topical steroids (dexamethasone, prednisolone, and fluorometholone) can reduce both hemangiogenesis and lymphangiogenesis to different degrees. 82 Some studies also looked into the relationship between the effect and timing of steroid administration.…”

Section: Steroidmentioning

confidence: 99%

Corneal neovascularization and contemporary antiangiogenic therapeutics

Hsu

Chang

Lin

et al. 2015

Journal of the Chinese Medical Association

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Corneal neovascularization (NV), the excessive ingrowth of blood vessels from conjunctiva into the cornea, is a common sequela of disease insult that can lead to visual impairment. Clinically, topical steroid, argon laser photocoagulation, and subconjunctival injection of bevacizumab have been used to treat corneal NV. Sometimes, the therapies are ineffective, especially when the vessels are large. Large vessels are difficult to occlude and easily recanalized. Scientists and physicians are now dedicated to overcoming this problem. In this article, we briefly introduce the pathogenesis of corneal NV, and then highlight the existing animal models used in corneal NV research-the alkali-induced model and the suture-induced model. Most of all, we review the potential therapeutic targets (i.e., vascular endothelial growth factor and platelet-derived growth factor) and their corresponding inhibitors, as well as the immunosuppressants that have been discovered in recent years by corneal NV studies.

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“…They reduce inflammatory cell infiltration and stabilize neutrophilic cytoplasmic and lysosomal membranes. They also help resolving anterior chamber as well as conjunctival inflammation [69]. The downside is that they also inhibit reepithelialization and collagen synthesis.…”

Section: Management Of Chemical Burnsmentioning

confidence: 99%

The Ocular Surface Chemical Burns

Eslani

Baradaran‐Rafii

Movahedan

et al. 2014

Journal of Ophthalmology

113

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Ocular chemical burns are common and serious ocular emergencies that require immediate and intensive evaluation and care. The victims of such incidents are usually young, and therefore loss of vision and disfigurement could dramatically affect their lives. The clinical course can be divided into immediate, acute, early, and late reparative phases. The degree of limbal, corneal, and conjunctival involvement at the time of injury is critically associated with prognosis. The treatment starts with simple but vision saving steps and is continued with complicated surgical procedures later in the course of the disease. The goal of treatment is to restore the normal ocular surface anatomy and function. Limbal stem cell transplantation, amniotic membrane transplantation, and ultimately keratoprosthesis may be indicated depending on the patients' needs.

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Clinical and Histopathological Outcomes of Subconjunctival Triamcinolone Injection for the Treatment of Acute Ocular Alkali Burn in Rabbits

Abstract: Subconjunctival injection of triamcinolone may be a therapeutic option for the treatment of acute ocular burn because it reduced the corneal inflammatory process, opacity, and vascularization, with no apparent clinical changes in the general state of the animal.

Cited by 28 publications

References 27 publications

Differential Effects of Dexamethasone and Doxycycline on Inflammation and MMP Production in Murine Alkali-Burned Corneas Associated with Dry Eye

Differential Effects of Dexamethasone and Doxycycline on Inflammation and MMP Production in Murine Alkali-Burned Corneas Associated with Dry Eye

Corneal neovascularization and contemporary antiangiogenic therapeutics

The Ocular Surface Chemical Burns

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