Clinical and genetic characterization of measles viruses isolated from adult patients in Shanghai in 2006

Yu, Xuelian; Qian, Fangxin; Sheng, Yinzhong; Xie, Desheng; Li, Dan; Huang, Qin; Zhang, Yong; Yuan, Zhenghong; Ghildyal, Reena

doi:10.1016/j.jcv.2007.07.004

Cited by 6 publications

(9 citation statements)

References 12 publications

(10 reference statements)

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“…Consistent with observations from other countries, 3 there were outbreaks of measles every 3-4 years in Shandong Province. 22 After implementation of measles vaccination activities, especially programme immunity, the number of epidemics decreased and incidence declined quickly.…”

Section: Discussionmentioning

confidence: 99%

Epidemic trend of measles in Shandong Province, China, 1963–2005

Kang

Zhang

et al. 2012

Public Health

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Section: Discussionmentioning

confidence: 99%

Epidemic trend of measles in Shandong Province, China, 1963–2005

Kang

Zhang

et al. 2012

Public Health

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“…MV was isolated from one of the patient samples using standard protocols and was used throughout the study (28,29). In brief, a sample of patient urine (50 -100 ml) was centrifuged (1500 rpm, 30 min) within 4 h of collection, the pellet resuspended in virus transport medium (DMEM containing 2% FBS, with 1000 U/ml penicillin and 1000 g/ml streptomycin), and used to inoculate semiconfluent Vero-SLAM cell monolayers grown in DMEM containing 10% FBS.…”

Section: Virus and Cell Culturementioning

confidence: 99%

Measles Virus Infection in Adults Induces Production of IL-10 and Is Associated with Increased CD4+CD25+ Regulatory T Cells

Cheng

Shi

et al. 2008

The Journal of Immunology

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Despite steady progress in elimination of measles virus globally, measles infection still causes 500,000 annual deaths, mostly in developing countries where endemic measles strains still circulate. Many adults are infected every year in China, with symptoms more severe than those observed in children. In this study, we have used blood samples from adult measles patients in Shanghai and age-matched healthy controls to gain an understanding of the immune status of adult measles patients. IFN-α mRNA was reduced in patient PBMC compared with healthy controls. In contrast, gene expression and plasma production of IL-2, IL-10, and IFN-γ were elevated in patient blood. A similar cytokine profile was observed at early times when cultured PBMC were infected with a clinical isolate of measles virus. In contrast to previous studies in pediatric patients, we did not find a reduction in total CD4+ and CD8+ T cells in patient PBMC. Interestingly, we found that CD4+CD25+CD127low regulatory T cells were significantly increased in patient PBMC compared with controls. Using intracellular cytokine staining we also show that the measles virus induces IL-10-producing CD14+ and CD4+CD25+ cells in PBMC. Our results show that adult measles patients in the acute phase of the disease have a mixed Th1/Th2 type response, accompanied with severe immunosuppression of both innate and adaptive responses including suppression of type I IFN. Both regulatory T cells and plasma IL-10 may contribute to the immunosuppression.

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“…This was the first time that an H1 MV was detected in Iran and the Eastern and 3). The Iranian H1 genotype was compared with different H1 sequences reported previously Yu et al, 2007]. The nucleotide and amino acid sequence homology with the H1a reference strain (China 93/2) was 98.9% and 97.3%; with the H1b reference strain (China 93/7) was 97.8% and 96%; with H1c reference strain (China 94/7) was 96.9% and 96% and with H2 reference strain (China 94/1) was 92.9% and 91.3% (Table II).…”

Section: Resultsmentioning

confidence: 99%

Molecular epidemiology of measles virus in Iran 2009–2010: First detection of measles genotype H1

Salimi

Mokhtari‐Azad

Abbasi

et al. 2011

Journal of Medical Virology

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Measles virus (MV) genotyping is an important component of measles surveillance in the context of monitoring immunization program effectiveness and documenting MV elimination. The molecular epidemiology and genetic variability of circulating MV strains in Iran during the 2009-2010 were studied in consecutive MV isolates from throat swab and urine. Sequence information obtained from 41 cases based on the 456 nucleotides of the most variable region of the C-terminal part of the N-protein revealed that these sequences belonged to two different genotypes. This is the first description of the genetic characterization of sporadic MV genotype H1 cases in northern Iran. Cases were probably linked to MV importation from distant parts of Asia. The genotype H1 has not been detected in the Eastern Mediterranean Region. In addition, both sequence analysis and epidemiologic data indicated that the more recently detected genotype D4 viruses in Iran were related very closely to viruses that were detected in Pakistan, suggesting that these viruses may have been imported from Pakistan. J. Med. Virol. 83:2200-2207, 2011. © 2011 Wiley Periodicals, Inc.

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Clinical and genetic characterization of measles viruses isolated from adult patients in Shanghai in 2006

Cited by 6 publications

References 12 publications

Epidemic trend of measles in Shandong Province, China, 1963–2005

Epidemic trend of measles in Shandong Province, China, 1963–2005

Measles Virus Infection in Adults Induces Production of IL-10 and Is Associated with Increased CD4+CD25+ Regulatory T Cells

Molecular epidemiology of measles virus in Iran 2009–2010: First detection of measles genotype H1

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